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pET His6 Mocr TEV cloning vector with BioBrick polycistronic restriction sites (9O)

Plasmid

#48289

Depositor

Scott Gradia

Type

Empty backbone

Tags

His6 and Mocr

Expression

Bacterial

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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evoBxb1

Plasmid

#222338

Purpose

Plasmid for expressing evolved Bxb1 in the mammalian cells

Depositor

David Liu

Publication

Pandey et al Nat Biomed Eng. 2024 Jun 10. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Codon-optimized evolved Bxb1

Expression

Mammalian

Promoter

CMV

Available since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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ARR3tk-eGFP/SV40-mCherry

Plasmid

#132360

Purpose

To measure transcriptional activity of Androgen Receptor

Depositor

Charles Sawyers

Publication

Lee et al Elife. 2019 Jan 15;8. pii: 41913. doi: 1

Insert

AR responsive elements (AR Human)

Use

Lentiviral

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pHAGE-N-FLAG-HA-TBK1

Plasmid

#131791

Purpose

Lentiviral vector expressing WT TBK1

Depositor

Alicia Pickrell

Publication

Sarraf et al Cell Rep. 2019 Oct 1;29(1):225-235.e5. d

Insert

TBK1 WT (TBK1 Human)

Use

Lentiviral

Tags

N-FLAG-HA

Expression

Mammalian

Mutation

WT full length

Promoter

CMV

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMGS46 (ARF16-PB1-HA-P2A-OsTIR1)

Plasmid

#126580

Purpose

A repair construct to express ARF16-PB1-HA and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

Depositor

Michael Guertin

Publication

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

OsARF16-PB1 domain

Tags

HA

Expression

Mammalian

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE

Plasmid

#122151

Purpose

Expresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.

Depositor

Clifford Brangwynne

Publication

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

dCas9 fused to SunTag(24x)

Use

Lentiviral

Tags

2xNLS-P2A-BFP and NLS

Expression

Mammalian

Promoter

SFFV

Available since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pHR-pSFFV-dCas9-SunTag-P2A-BFP-dWPRE

Plasmid

#122151

Purpose

Expresses dCas9-SunTag(24X) fusion and a fluorescent indicator BFP.

Depositor

Clifford Brangwynne

Publication

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

dCas9 fused to SunTag(24x)

Use

Lentiviral

Tags

2xNLS-P2A-BFP and NLS

Expression

Mammalian

Promoter

SFFV

Available since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pHAGE-BRAF-V600E

Plasmid

#116204

Purpose

Lentiviral expression of BRAF V600E

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

V600E

Available since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pHAGE-BRAF-V600E

Plasmid

#116204

Purpose

Lentiviral expression of BRAF V600E

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

BRAF (BRAF Human)

Use

Lentiviral

Mutation

V600E

Available since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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LbCpf1-2NLS

Plasmid

#102566

Purpose

bacterial expression of LbCpf1-2NLS

Depositor

Jennifer Doudna

Publication

Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

Insert

LbCpf1

Tags

6xHis-MBP-TEV and HA-2xSV40NLS

Expression

Bacterial

Promoter

T7

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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LbCpf1-2NLS

Plasmid

#102566

Purpose

bacterial expression of LbCpf1-2NLS

Depositor

Jennifer Doudna

Publication

Moreno-Mateos et al Nat Commun. 2017 Dec 8;8(1):2024. doi: 1

Insert

LbCpf1

Tags

6xHis-MBP-TEV and HA-2xSV40NLS

Expression

Bacterial

Promoter

T7

Available since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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TroponinT-GCaMP5-Zeo

Plasmid

#46027

Depositor

John Gearhart

Publication

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Insert

GCaMP5-2A-Zeo

Use

Lentiviral

Expression

Mammalian

Promoter

TNNT2

Available since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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TroponinT-GCaMP5-Zeo

Plasmid

#46027

Depositor

John Gearhart

Publication

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Insert

GCaMP5-2A-Zeo

Use

Lentiviral

Expression

Mammalian

Promoter

TNNT2

Available since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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GFP-MAP1B

Plasmid

#44396

Depositor

Phillip Gordon-Weeks

Publication

Scales et al J Cell Sci. 2009 Jul 15;122(Pt 14):2424-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MAP1B (Map1b Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

May 21, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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GFP-MAP1B

Plasmid

#44396

Depositor

Phillip Gordon-Weeks

Publication

Scales et al J Cell Sci. 2009 Jul 15;122(Pt 14):2424-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MAP1B (Map1b Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

May 21, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLCA.66/2272

Plasmid

#22733

Depositor

Mark Magnuson

Publication

Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

Insert

Lox66-pU(delta)TK-EM7Neo-Lox2272

Use

Cre/Lox; Bac recombineering

Mutation

This vector was designed for assembling gene targ…

Available since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLCA.66/2272

Plasmid

#22733

Depositor

Mark Magnuson

Publication

Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

Insert

Lox66-pU(delta)TK-EM7Neo-Lox2272

Use

Cre/Lox; Bac recombineering

Mutation

This vector was designed for assembling gene targ…

Available since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pET His6 Gamma crystallin TEV LIC cloning vector (1X)

Plasmid

#29661

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, Gamma-crystallin, and TEV cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pABE8e-protein

Plasmid

#161788

Purpose

Rhamnose-inducible expression of 8xHIS-ABE8e in E. coli

Depositor

David Liu

Publication

Huang et al Nat Protoc. 2021 Feb;16(2):1089-1128. do

Insert

ABE8e base editor

Tags

8xHIS and BPNLS

Expression

Bacterial

Promoter

pRhaBAD

Available since

June 22, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDONR207 SARS-CoV-2 NSP1

Plasmid

#141255

Purpose

Gateway-compatible Entry vector, with insert of NSP1 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP1 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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