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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,457 results for: nts

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Showing: 3041 - 3060 of 12457 results

  3. pAAV.hSynapsin.SF-Venus-iGluSnFR.A184V

    Plasmid
    #106178
    Purpose
    Medium affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **
    Depositor
    Loren Looger
    Article
    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do
    Insert
    SF-Venus-iGluSnFR.A184V
    Use
    AAV
    Mutation
    SFGFP: T203Y, F46L, T65G, S72A GltI: A184V
    Promoter
    hSynapsin
    Available Since
    Feb. 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    106178-plasmid-map-sequence-id-204208

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  7. huCof WT SSR RedTrack

    Plasmid
    #245955
    Purpose
    Expression of WT coifilin resistant to shRNA in cells coexpressing mRFP
    Depositor
    James Bamburg
    Insert
    cofilin 1 (cfl1) (CFL1 Human)
    Expression
    Mammalian
    Mutation
    silent mutations (NTs 67 to 75) in which TCTTCAAC…
    Promoter
    CMV for huCof WT and separate CMV for mRFP
    Available Since
    Oct. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    245955-plasmid-map-sequence-id-490153

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  8. huCofilin S3E SSR

    Plasmid
    #245940
    Purpose
    Expresses human cofilin with mutation S3E that behaves like inactive phosphocofilin but can be dominant negative against phosphatase
    Depositor
    James Bamburg
    Article
    Agnew et al J Biol Chem. 1995 Jul 21;270(29):17582-7
    Insert
    cofilin 1 (cfl1) (CFL1 Human)
    Expression
    Mammalian
    Mutation
    S3E; silent mutations (NTs 67 to 75) in which TCT…
    Promoter
    CMV
    Available Since
    Oct. 21, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    245940-plasmid-map-sequence-id-490033

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  9. P22_wtCP_SP141his

    Plasmid
    #122651
    Purpose
    Expression of P22 virus like particle in bacteria
    Depositor
    Trevor Douglas
    Insert
    P22 Coat: WT / SP: 6xHis-SP141
    Expression
    Bacterial
    Mutation
    truncated SP, P120H in the capsid scaffold (see …
    Available Since
    Aug. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    122651-plasmid-map-sequence-id-237561

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  14. DDX5A

    Plasmid
    #42421
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    DDX5A (DDX5 Human)
    Tags
    His6-TEV
    Expression
    Bacterial
    Mutation
    See Comments
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. pLJM1-Lyso-FLAG-GFP-Sac1-Cat-CS

    Plasmid
    #134653
    Purpose
    lysosome-targeted Sac1 catalytic domain C389S
    Depositor
    Roberto Zoncu
    Article
    Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218
    Insert
    Sac1 catalytic domain (77-520 aa) CS mutant (SACM1L Human)
    Use
    Lentiviral
    Tags
    FLAG, GFP, and lysosomal-tag (p18 N-terminal 1-39…
    Expression
    Mammalian
    Mutation
    C389S and contains only amino acids 77-520 (Pleas…
    Available Since
    Dec. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    134653-plasmid-map-sequence-id-266494

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Showing: 3041 - 3060 of 12457 results