We narrowed to 39,188 results for: NAM

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pHybrid-Electra1

Plasmid

#179474

Purpose

Expresses Electra1 in bacterial cells and Electra1 and mScarlet in mammalian cells

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Inserts

Electra1
mScarlet-I

Expression

Bacterial and Mammalian

Available Since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pDonor_RB-TnV2

Plasmid

#213907

Purpose

Barcoded mariner transposon with kan selection casette, and modified terminal sequences - barcodes can be added via FseI-SbfI restriction sites

Depositor

James Collins

Article

English et al Mol Syst Biol. 2023 Jun 12;19(6):e11398.

Insert

mariner transposon with kan resistance and FseI-SbfI sites for barcode insertion

Expression

Bacterial

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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PX552-rScn2a-3xV5-Syn-miRFP670

Plasmid

#182566

Purpose

For rat Nav1.2 knockin with 3xV5 at the extracellular loop

Depositor

James Zhe Liu

Article

Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

Inserts

rat Scn2a gRNA and 3xV5 donor DNA
miRFP670

Use

AAV and CRISPR

Promoter

Syn and U6

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pDZ617 pKAN 12xPP7 V4

Plasmid

#72237

Purpose

Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

12xPP7 V4

Use

Cre/Lox

Expression

Bacterial

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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P336-FBP_11

Plasmid

#139702

Purpose

T-DNA encoding Fungal Bioluminescent Pathway Components, control pathway, missing Luz

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNPGA:tAtug7, pCmYLCV:NnH3H:AtuOcs, pCmYLCV:NnHisps:tHsp

Use

Luciferase

Expression

Plant

Promoter

various

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCpf1b

Plasmid

#122187

Purpose

Bacterial genome editing plasmid with kanamycin resistance marker and using sacB as a counter-selection marker

Depositor

Cheng-Cai Zhang

Article

Niu et al ACS Synth Biol. 2018 Dec 10. doi: 10.102

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoB-NAD

Plasmid

#193824

Purpose

CMV overexpression of ChemoB-NAD in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoB-NAD

Expression

Mammalian

Mutation

EBFP2:N39Y, A206K, T225R; HT7: L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pDonor14-AcHELIX-KanR (CJT96)

Plasmid

#181794

Purpose

pDonor for AcHELIX containing 14bp of spacing between the I-AniI site and LE/RE using ShCAST flanking sequence and encoding a kanamycin resistance gene as cargo

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

KanR, R6K origin of replication

Expression

Bacterial

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pANCH2-Nat

Plasmid

#87249

Purpose

gene for resistance to nourseothricin (Nat) inserted next to the parS segments (INT)

Depositor

Kerstin Bystricky

Article

Saad et al PLoS Genet. 2014 Mar 13;10(3):e1004187.

Insert

Nat

Expression

Yeast

Available Since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

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pDZ645 pKAN 1x-mCherry-12xPP7 V4

Plasmid

#73173

Purpose

Floxed Kanamycin (G418) selection maker; 3' tagging vector used to add 1x-mCherry followed by 12 repetitive PP7 version 4 RNA stem-loops to gene of interest

Depositor

Daniel Zenklusen

Article

Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

Insert

1x-mCherry

Expression

Mammalian and Yeast

Available Since

Aug. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pEBTet_NAD-Snifit-mito

Plasmid

#113916

Purpose

Plasmid encoding the mitochondrial localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cells

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NAD-Snifit

Expression

Mammalian

Promoter

CMV TetO2

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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PP_079_pAAV_CAG_LR-Voltron2-P2A-CheRiff-eGFP-ST (Kv2.1TS)

Plasmid

#230989

Purpose

Co-expressing membrane-localized Voltron2 and soma-localized CheRiff.

Depositor

Adam Cohen

Article

Wong-Campos et al bioRxiv [Preprint]. 2023 May 26:2023.05.

Insert

Membrane-localized Voltron2 and soma-localized CheRiff

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoG-ATP

Plasmid

#193818

Purpose

CMV overexpression of ChemoG-ATP in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG-ATP

Expression

Mammalian

Mutation

EGFP:A206K; HT7: L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/Puro-CAG-JEDI-1P

Plasmid

#202607

Purpose

Genetically encoded voltage indicator (GEVI) JEDI-1P, expressed under strong mammalian promoter (CAG)

Depositor

Francois St-Pierre

Article

Lu et al Nat Commun. 2023 Oct 12;14(1):6423. doi:

Insert

JEDI-1P

Expression

Mammalian

Promoter

CAG

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pHR iSH-YFP-PIF

Plasmid

#50841

Purpose

Optogenetic PI3K activation system; cytoplasmic component

Depositor

Wendell Lim

Article

Toettcher et al Nat Methods. 2011 Sep 11;8(10):837-9. do

Inserts

p85 iSH2
PIF6 1-100

Use

Lentiviral and Synthetic Biology

Available Since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pCOLA-Gent-EM7-Erv1p

Plasmid

#202484

Purpose

Expresses sulfhydryl oxidase (Erv1p) constitutively

Depositor

Michael Lynch

Article

Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

Insert

sulfydryl oxidase (Erv1p) (ERV1 Budding Yeast)

Expression

Bacterial

Promoter

EM7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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plenti.CamKII.(cyto).iATPSnFR2.A95K.HaloTag

Plasmid

#209726

Purpose

Expresses cytosolic medium affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

plenti.CamKII.(cyto).iATPSnFR2.A95K.HaloTag

Use

Lentiviral

Expression

Mammalian

Promoter

CamKII

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoG-CaM

Plasmid

#193816

Purpose

CMV overexpression of ChemoG-CaM in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoG-CaM

Expression

Mammalian

Mutation

EGFP:E116D, A206K; HT7: L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pEBTet_NAD-Snifit-cyto

Plasmid

#113914

Purpose

Plasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cells

Depositor

Kai Johnsson

Article

Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

Insert

NAD-Snifit

Expression

Mammalian

Promoter

CMV TetO2

Available Since

Sept. 19, 2018

Availability

Academic Institutions and Nonprofits only

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GW1-mCherryEA

Plasmid

#207867

Purpose

Mammalian expression (cytosol) of the mCherry(I158E/Q160A) mutant as a red fluorescent pH sensor

Depositor

Mathew Tantama

Article

Rajendran et al ACS Omega. 2018 Aug 31;3(8):9476-9486. d

Insert

mCherryEA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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