We narrowed to 31,202 results for: uros

1. pRP(Exp)-CMV> ORF_924bp (Azgp1):T2A:dTomato:IRES:Puro

   Plasmid

   #110830

   Purpose

   Expresses zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter where both sequences are separated by a T2A self-cleaving peptide sequence as a result, the dT is not fused with the ZAG protein.

   Depositor

   Ande Satyanarayana

   Article

   Elattar et al FASEB J. 2018 Mar 23:fj201701465RR. doi:

   Insert

   AZGP1 (Azgp1 Mouse)
   

   Use

   Non-viral gene expression vector

   Tags

   T2A and dTomato

   Expression

   Mutation

   Promoter

   CMV

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSUPER-retro-puro-tetO-CTR1-shRNA

   Plasmid

   #53180

   Purpose

   Expresses shRNA against human CTR1 from puromycin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   CTR1 (SLC31A1 Human)
   

   Use

   RNAi and Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1

   Plasmid

   #164800

   Purpose

   For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   ERH CRISPRi gRNA1
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1Alpha and U6

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2

   Plasmid

   #164801

   Purpose

   For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2

   Depositor

   David Bartel

   Article

   Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

   Inserts

   ERH CRISPRi gRNA2
   puro-T2A-BFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1Alpha and U6

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti CMV Puro DEST (w118-1) VOPP1

   Plasmid

   #107507

   Purpose

   expressing VOPP1, puromycin resistant

   Depositor

   Alejandro Gutierrez

   Article

   Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

   Insert

   VOPP1 (VOPP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRetroX-Tight-puro-3xFLAG-RUNX3 R122C

   Plasmid

   #203439

   Purpose

   Mammalian expression of RUNX4

   Depositor

   Yoshiaki Ito

   Article

   Oei et al Commun Biol. 2023 Jul 3;6(1):689. doi: 1

   Insert

   RUNX3 (RUNX3 Human)
   

   Use

   Tags

   3 x FLAG

   Expression

   Mammalian

   Mutation

   R122C

   Promoter

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. P932_CLYBL-CBX3-Cbh-Zeo-TetO-NEUROG2

   Plasmid

   #202762

   Purpose

   Encodes one forebrain neuron specific transcription factor under the control of a TetOn promoter at the CLYBL safe harbor

   Depositor

   Karl Wahlin

   Article

   Agarwal et al NPJ Regen Med. 2023 Sep 29;8(1):55. doi:

   Insert

   NEUROG2 (NEUROG2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TetO

   Available since

   Feb. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. lenti-CMV-TFAM-BioID2-IRES-Puro

   Plasmid

   #211465

   Purpose

   Lentivirus backbone expressing human TFAM fused with BioID2

   Depositor

   Agnel Sfeir

   Article

   Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

   Insert

   TFAM-BioID2
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-C-Myc-DDK-IRES-Puro HNRNPR

   Plasmid

   #200730

   Purpose

   Lentiviral expression of Flag-Tagged human HNRNPR

   Depositor

   Claudio Alarcón

   Insert

   HNRNPR (HNRNPR Human)
   

   Use

   Lentiviral

   Tags

   Myc-DDK from backbone plasmid

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pU6-mir451 EF1Alpha-puro-T2A-BFP

    Plasmid

    #164792

    Purpose

    Express miR-451 under the U6 promoter in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    microRNA 451a (MIR451A Human)
    puro-T2A-BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1Alpha and U6

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. nonphosphorylatable Mis12-targeted Aurora B FRET sensor

    Plasmid

    #45229

    Depositor

    Michael Lampson

    Article

    Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

    Insert

    non phosphorylatable Mis12-targeted Aurora B FRET sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T-A mutation in substrate sequence

    Promoter

    Available since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1-puro U6 sgRNA BfuAI stuffer G-tract

    Plasmid

    #138524

    Purpose

    Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt PRC2-binding G-tract repeat RNA sequence.

    Depositor

    Richard Jenner

    Article

    Beltran et al Nat Struct Mol Biol. 2019 Oct;26(10):899

    Insert

    PRC2-binding G-tract repeat RNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. mCherry-Cb5-IRES-puromycin-pLVx-EF1a

    Plasmid

    #177434

    Purpose

    To express mCherry fused to Cb5 endoplasmic reticulum (ER) targeting sequence. Lentiviral vector used to make cell lines expressing this ER landmark.

    Depositor

    David Andrews

    Insert

    Cb5 tail anchor (Cyb5r4 Rat)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti CMV Puro DEST (w118-1) FAM149A

    Plasmid

    #107510

    Purpose

    Expressing FAM149a, puromycin resistant

    Depositor

    Alejandro Gutierrez

    Article

    Aries et al J Exp Med. 2018 Nov 7. pii: jem.20180570

    Insert

    FAM149A (FAM149A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HA-HIF2α P405A/P531A/N847Q-pBabe-Puro

    Plasmid

    #25948

    Depositor

    William Kaelin

    Article

    Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

    Insert

    Hypoxia inducible factor 2 alpha (EPAS1 Human)
    

    Use

    Retroviral

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    cDNA changed amino acids: Proline 405 to Alanin…

    Promoter

    Available since

    Sept. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. piggyBac_rtTA (4th_Gen)_Gateway_IRES_NGN2-2A-PURO_Synapsin-BirA. (pEHA1618)

    Plasmid

    #209084

    Purpose

    Ngn2 mediated cortical neuron induction, gateway destination, Synapsin promoter BirA ligase expression

    Depositor

    Vikram Khurana

    Article

    Hallacli et al Cell. 2022 Jun 9;185(12):2035-2056.e33.

    Inserts

    NGN2 (Neurog2 Mouse)
    BirA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    hSyn

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-CAG-FLEX-NEUROD1-T2A-mCherry

    Plasmid

    #178584

    Purpose

    Cre-dependent expression of NEUROD1 and mCherry under the constitutive promoter

    Depositor

    Chun-Li Zhang

    Article

    Wang et al Cell. 2021 Oct 14;184(21):5465-5481.e16.

    Insert

    NEUROD1 (NEUROD1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCDH-CB-FLPe-P2A-copGFP-T2A-Puro

    Plasmid

    #72258

    Purpose

    Express FLPe, copGFP and Puromycin resistance gene under CB promoter

    Depositor

    Kazuhiro Oka

    Insert

    FLPe-P2A-copGFp-T2A-Puro
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CB and chicken beta-actin + CMV enhancer

    Available since

    Jan. 15, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. Direct-seq_lentiGuide-Puro-tRNA-Tail-8A8G

    Plasmid

    #157984

    Purpose

    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

    Depositor

    Lijia Ma

    Article

    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

    Insert

    S. pyogenes sgRNA cassette (tRNA-Tail-8A8G)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-C-Myc-DDK-IRES-Puro LARP7

    Plasmid

    #200725

    Purpose

    Lentiviral expression of Flag-Tagged human LARP7

    Depositor

    Claudio Alarcón

    Insert

    LARP7 (LARP7 Human)
    

    Use

    Lentiviral

    Tags

    Myc-DDK from backbone plasmid

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge