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Showing: 3061 - 3080 of 3209 results

  1. p3E-Lact-C2

    Plasmid
    #109553
    Purpose
    Multisite gateway vector for 3' tagging with the C-terminal domain from cow (Bos taurus) milk fat globue EGF-factor 8 protein (MFGE8). Binds phosphatidylserine (PS)
    Depositor
    Rob Parton
    Article
    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:
    Insert
    the C-terminal domain from cow (Bos taurus) milk fat globue EGF-factor 8 protein (MFGE8).
    Use
    Zebrafish plasmids
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    109553-plasmid-map-sequence-id-222017

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  2. pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)

    Plasmid
    #181745
    Purpose
    dual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1
    Depositor
    Benjamin Kleinstiver
    Article
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Insert
    human/zebrafish codon optimized SpCas9
    Use
    In vitro transcription; t7 promoter
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181745-plasmid-map-sequence-id-354676

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  8. p3E-DrCav1

    Plasmid
    #194290
    Purpose
    Multisite gateway entry clone for expression of codon optimised zebrafish caveolin1 with fusion tag at the N-terminus. Parton lab clone KRT
    Depositor
    Rob Parton
    Article
    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.
    Insert
    caveolin1 (cav1.S Frog)
    Use
    Multisite gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    194290-plasmid-map-sequence-id-380039

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  9. pCS2+ cMyc-oDam_f_GFPoNLS

    Plasmid
    #85818
    Purpose
    iDamID plasmid. To express transiently the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Joachim Wittbrodt
    Article
    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261
    Insert
    cMyc oDam_f_GFPoNLS
    Use
    Tags
    cMyc and oNLS (optimized nuclear localization sig…
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    L122A. Codon optimization compatible to work in M…
    Promoter
    Available since
    Jan. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    85818-plasmid-map-sequence-id-169572

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  10. CmCitrine Lck w/ NosUTRs

    Plasmid
    #66785
    Purpose
    expression of fluorescent membranes in sea urchin primordial germ cells
    Depositor
    Amro Hamdoun
    Article
    Campanale et al Dev Dyn. 2014 Jul;243(7):917-27. doi: 10
    Insert
    Lck (LCK Human)
    Use
    Sea urchin, zebrafish, xenopus
    Tags
    3' UTR from sea urchin Nanos, 5' UTR fr…
    Expression
    Mutation
    Tandem repeat of LCK the N-terminal membrane targ…
    Promoter
    T7
    Available since
    July 24, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. pLL5.0_shRNAHsCavin1_Del4UC1_EGFP

    Plasmid
    #229691
    Purpose
    Lentiviral expression of shRNA targeting Cavin1 + reconstitution of zebrafish mutant Cavin1 (Del4UC1)
    Depositor
    Alpha Yap
    Article
    Brooks et al Mol Biol Cell. 2023 Nov 1;34(12):ar120.
    Inserts
    shRNA for hCavin1 (CAVIN1 Human)
    mutant cavin1b (Del4UC1) (cavin1b Zebrafish)
    Use
    Lentiviral
    Tags
    EGFP
    Expression
    Mutation
    Del4UC1
    Promoter
    LTR viral promoter
    Available since
    Jan. 29, 2025
    Availability
    Academic Institutions and Nonprofits only
    229691-plasmid-map-sequence-id-459544

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  13. pCS2-MfnG-EcYRS-EGFP*

    Plasmid
    #191119
    Purpose
    A plasmid for incorporation of O-Me-Tyr into an EGFP reporter without O-Me-Tyr feeding for zebrafish
    Depositor
    Han Xiao
    Article
    Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:
    Insert
    MfnG - P2A - Mutant E. coli TyrRS - T2A - enhanced green fluorescence protein
    Use
    Tags
    His tag
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    191119-plasmid-map-sequence-id-373722

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  15. p3E-DrCavin1b

    Plasmid
    #194292
    Purpose
    Multisite gateway entry clone for expression of codon optimised zebrafish cavin1b with fusion tag at the N-terminus. Parton lab clone KXJ
    Depositor
    Rob Parton
    Article
    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.
    Insert
    cavin1b (cavin1b Zebrafish)
    Use
    Multisite gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    194292-plasmid-map-sequence-id-380042

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Showing: 3061 - 3080 of 3209 results