Skip to main content
Addgene
Showing: 6161 - 6200 of 6265 results

  1. AID* Kit

    Plasmid Kit
    #1000000120
    Purpose
    Expanded tool kit for the auxin-inducible degron (AID) system in yeast ( S. cerevisiae). Includes tags for easy detection by antibodies/microscopy and a set of selection markers.
    Depositor
    Helle Ulrich
    Available since
    Jan. 10, 2018
    Availability
    Academic Institutions and Nonprofits only

  2. biGBac Kit

    Plasmid Kit
    #1000000088
    Purpose
    Generation of baculoviral transfer vectors coding up to 25 protein complex subunits in two Gibson assembly steps. Multisubunit protein complexes can be generated in baculovirus-infected insect cells.
    Depositor
    Jan-Michael Peters
    Available since
    Oct. 25, 2016
    Availability
    Academic Institutions and Nonprofits only

  3. rBiFC Kit

    Plasmid Kit
    #1000000147
    Depositor
    Christopher Grefen
    Available since
    March 11, 2019
    Availability
    Academic Institutions and Nonprofits only

  4. CyanoGate Kit

    Plasmid Kit
    #1000000146
    Purpose
    The CyanoGate kit contains 96 parts and acceptor vectors for generating multi-part assemblies in either integrative (suicide) or self-replicating plasmid vectors for working in cyanobacteria.
    Depositor
    Alistair McCormick
    Available since
    Jan. 14, 2020
    Availability
    Academic Institutions and Nonprofits only

  5. CRISPi Kit

    Plasmid Kit
    #1000000136
    Purpose
    CRISPi Kit contains elements for CRISPR/Cas9 mediated genome editing in yeast P. pastoris. Contains 7 different backbone (BB3) vectors with Cas9/ sgRNA expression cassettes with NTC, hphMX, or kanMX.
    Depositor
    Brigitte Gasser
    Available since
    Aug. 27, 2018
    Availability
    Academic Institutions and Nonprofits only

  6. GoldenPiCS Kit

    Plasmid Kit
    #1000000133
    Purpose
    GoldenPiCS: plasmid kit for engineering in P. pastoris (GoldenMOCS based). Overexpression of up to eight transcription units. 20 promoters, 10 terminators, 4 selection markers and 4 integration sites
    Depositor
    Brigitte Gasser
    Available since
    Feb. 20, 2018
    Availability
    Academic Institutions and Nonprofits only

  7. SapTrap Kit

    Plasmid Kit
    #1000000077
    Purpose
    SapTrap: modular toolkit for building CRISPR/Cas9 targeting vectors to insert genetic tags in C. elegans genome. 21 vector assembly plasmids, Unc-32::GFP targeting vector, 5 Cre/Flp expression vectors
    Depositor
    Erik Jorgensen
    Available since
    March 25, 2016
    Availability
    Academic Institutions and Nonprofits only

  8. ScEnSor Kit

    Plasmid Kit
    #1000000215
    Purpose
    This three-step (Build - Transform - Assess) Saccharomyces cerevisiae kit is comprised of two modules (Engineering and Biosensor) that can be used independently or in combination.
    Depositor
    Lisbeth Olsson
    Available since
    May 8, 2023
    Availability
    Academic Institutions and Nonprofits only

  9. AspFlex Kit

    Plasmid Kit
    #1000000217
    Purpose
    This collection is a set of Modular Cloning (MoClo) plasmids for Acinetobacter spp. and Acinetobacter baumannii for protein expression analyses and CRISPRi experiments.
    Depositor
    Veronica Godoy-Carter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only

  10. CRISPRecise Kit

    Plasmid Kit
    #1000000235
    Purpose
    CRISPRecise is a set of high-fidelity SpCas9 variants that can be used to efficiently edit any SpCas9 target with practically no detectable off-target effects.
    Depositor
    Ervin Welker
    Available since
    Jan. 11, 2024
    Availability
    Academic Institutions and Nonprofits only

  11. TUNEYALI Kit

    Plasmid Kit
    #1000000255
    Purpose
    Use the TUNEYALI kit for precise regulation of transcription factor expression in Yarrowia lipolytica.
    Depositor
    Irina Borodina
    Available since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only

  13. CRIMPkit

    Plasmid Kit
    #1000000225
    Purpose
    The CRISPR/Cas9 Insertional Mutagenesis Protocol (CRIMP) and toolkit (CRIMPkit) is a universal and highly efficient targeted insertional mutagenesis system designed for use in zebrafish.
    Depositor
    Robert Bryson-Richardson
    Available since
    Aug. 29, 2023
    Availability
    Academic Institutions and Nonprofits only

  14. AdenoBuilder toolkit

    Plasmid Kit
    #1000000176
    Purpose
    Plasmids containing modular inserts that span the human adenovirus serotype 5 (HAdV5) genome that can be assembled in a one-tube reaction.
    Depositor
    Fred Bunz
    Available since
    Dec. 1, 2021
    Availability
    Academic Institutions and Nonprofits only

  15. The Mammalian Toolkit

    Plasmid Kit
    #1000000180
    Purpose
    A ready-made extensive library of plasmids that enable Golden Gate high-throughput assembly of genetic circuits that can be delivered to cells in a multitude of ways.
    Depositor
    Hana El-Samad
    Available since
    March 11, 2022
    Availability
    Academic Institutions and Nonprofits only

  16. EMMA toolkit

    Plasmid Kit
    #1000000119
    Purpose
    Used for the construction of a variety of mammalian expression vectors in a one-pot, one-step Golden Gate reaction; compatible with automated production
    Depositor
    Yizhi Cai
    Available since
    March 1, 2018
    Availability
    Academic Institutions and Nonprofits only

  17. FX cloning Kit

    Plasmid Kit
    #1000000039
    Purpose
    Insert sequence of interest into a cloning vector and subclone into expression vectors, containing a variety of N- and C-terminal tags; for use in both prokaryotic and eukaryotic systems
    Depositor
    Eric Geertsma
    Available since
    April 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. Ralstonia-GG-Kit

    Plasmid Kit
    #1000000142
    Purpose
    Backbone vectors and Level I modules that can be used for assembly of Ralstonia solanacearum species complex gene knockout and complementation constructs based on Golden Gate cloning techniques
    Depositor
    Thomas Lahaye
    Available since
    Dec. 18, 2018
    Availability
    Academic Institutions and Nonprofits only

  19. Plasmid-ID Kit

    Plasmid Kit
    #1000000168
    Purpose
    The Plasmid-ID Kit allows for the high-throughput assessment of competitive and effective rhizobial strains.
    Depositor
    Philip Poole
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only

  20. MoClo Yeast Toolkit

    Plasmid Kit
    #1000000061
    Purpose
    96 standardized parts used for bottom-up hierarchical assembly of single and multi-gene constructs for S. cerevisiae expression. Includes promoters, terminators, peptide tags, selection cassettes
    Depositor
    John Dueber
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only

  21. Cultivarium POSSUM Toolkit

    Plasmid Kit
    #1000000234
    Purpose
    The POSSUM Toolkit is a Golden Gate genetic toolkit developed to help researchers identify functional plasmids and establish genetic tractability in non-model microbes.
    Depositor
    Nili Ostrov
    Available since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only

  22. GreenGate 2.0 Toolkit

    Plasmid Kit
    #1000000241
    Purpose
    The GreenGate 2.0 (GG2.0) toolkit enables the assembly and stacking of complex transcriptional units with the GreenGate Cloning System. It is backwards compatible with most existing GreenGate modules.
    Depositor
    Alexis Maizel
    Available since
    April 11, 2024
    Availability
    Academic Institutions and Nonprofits only

  23. PRESTO-Tango Kit

    Plasmid Kit
    #1000000068
    Purpose
    Used to measure receptor activation for more than 300 G-protein coupled receptors (GPCRs) via a modified Tango beta-arrestin recruitment assay.
    Depositor
    Bryan Roth
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  24. MoClo Tool Kit

    Plasmid Kit
    #1000000044
    Purpose
    Eukaryotic multigene construct assembly in 3 steps using modular cloning (MoClo); Has 95 empty standardized genetic modules: promoters, 5' UTRs, signal peptides, coding sequences, and terminators
    Depositor
    Sylvestre Marillonnet
    Available since
    April 8, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  25. GoldenBraid 2.0 Kit

    Plasmid Kit
    #1000000076
    Purpose
    Modular cloning of multigenic DNA constructs in Plant Synthetic Biology applications. 95 GoldenBraid cloning compatible plasmids, containing standard basic parts and transcriptional units
    Depositor
    Diego Orzaez
    Available since
    March 16, 2016
    Availability
    Academic Institutions and Nonprofits only

  26. EcoFlex MoClo Kit

    Plasmid Kit
    #1000000080
    Purpose
    Golden Gate kit for E. coli synthetic biology; consists of a modular library that uses MoClo assembly standard for a variety of uses, e.g. recombinant protein purification, cell-free circuit design
    Depositor
    Paul Freemont
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  27. openCIDAR MoClo Toolkit

    Plasmid Kit
    #1000000227
    Purpose
    This collection includes broad host range, pBBR1-based vectors compatible with the CIDAR MoClo cloning standard. These vectors replicate in many bacteria across Pseudomonadota.
    Depositor
    Eric Young
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only

  28. pCut plasmid toolkit

    Plasmid Kit
    #1000000118
    Purpose
    Cas9-based kit in S. cerevisiae for optimizing heterologous gene expression. Cas9-sgRNA (pCut) plasmids for integrating into 23 loci, across all 16 yeast chromosomes.
    Depositor
    Aindrila Mukhopadhyay
    Available since
    Aug. 4, 2017
    Availability
    Academic Institutions and Nonprofits only

  29. Chromoprotein kit 1.0

    Plasmid Kit
    #1000000174
    Purpose
    A synthetic biology collection of plasmids for expression of chromoproteins in bacteria, which can be used as an educational resource.
    Depositor
    Anthony Forster
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only

  30. Chromoprotein kit 2.0

    Plasmid Kit
    #1000000198
    Purpose
    Chromoprotein plasmid kit that contain two new chromoprotein genes that are less toxic to E. coli so that the plasmids are more stable during growth.
    Depositor
    Anthony Forster
    Available since
    March 7, 2023
    Availability
    Academic Institutions and Nonprofits only

  31. MoClo Pichia toolkit

    Plasmid Kit
    #1000000108
    Purpose
    A collection of control elements and fluorescent reporters that can be used in combination with the MoClo-YTK toolkit for protein expression and secretion in yeast (Pichia pastoris).
    Depositor
    Volker Sieber
    Available since
    June 1, 2017
    Availability
    Academic Institutions and Nonprofits only

  32. Yeast Prototrophy Kit

    Plasmid Kit
    #1000000085
    Purpose
    Plasmids derived from the pHLUM mini-chromosome, designed to restore prototrophy (eg HIS3, LEU2, URA3, MET17 or LYS2 genes) in common yeast (S. cerevisiae) laboratory strains.
    Depositor
    Markus Ralser
    Available since
    Oct. 12, 2016
    Availability
    Academic Institutions and Nonprofits only

  33. PUF Assembly Kit

    Plasmid Kit
    #1000000051
    Purpose
    Used to assemble custom-made RNA-binding proteins for recognition of 8-base long targets within RNA. The kit is based on the Pumilio and FBF (PUF) homology domain of the full-length human Pumilio 1.
    Depositor
    Huimin Zhao
    Available since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  34. MXS Chaining Kit

    Plasmid Kit
    #1000000064
    Purpose
    MXS-chaining system for assembly of constructs optimized for synthetic biology applications in mammalian systems. 14 different fluorescent proteins, 10 mammalian promoters/enhancers, 3 polyA signals
    Depositor
    Pierre Neveu
    Available since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  35. pGCS vector kit

    Plasmid Kit
    #1000000107
    Purpose
    A series of pCS2+ based Gateway vectors with N- or C-terminal tags (eg Myc, HA, Flag, GST and eGFP), which allow the generation of tagged proteins in a variety of vertebrate organisms.
    Depositor
    Hai-Ning Du
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only

  36. FREQ-Seq Kit

    Plasmid Kit
    #1000000032
    Purpose
    For construction of barcoded, locus-specific libraries compatible with Illumina next generation sequencing. Allows for direct quantitative allele detection. 48 plasmid adaptors (barcoded M13F primer)
    Depositor
    Christopher Marx
    Available since
    May 23, 2013
    Availability
    Academic Institutions and Nonprofits only

  37. MoClo Baculo toolkit

    Plasmid Kit
    #1000000256
    Purpose
    Use the MoClo Baculo Toolkit to efficiently generate multigene expression constructs for the baculovirus expression system.
    Depositor
    Chen Davidovich
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only

  38. The Expanded CRISPEY Kit

    Plasmid Kit
    #1000000257
    Purpose
    Use the Expanded CRISPEY Kit for CRISPR-based gene editing of S. cerevisiae.
    Depositor
    Jeffrey Lewis
    Available since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only

  39. 4G-Cloning Plasmid Kit

    Plasmid Kit
    #1000000252
    Purpose
    The 4G (Golden-Gate-guided Gibson) cloning kit provides elements for rapid one-day assembly of multi-gene expression vectors in bacterial and eukaryotic expression hosts.
    Depositor
    Stephan Gruber
    Available since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only

  40. Ubiquiton Human Kit

    Plasmid Kit
    #1000000244
    Purpose
    The Ubiquiton system allows the inducible, linkage-specific polyubiquitylation of a protein of interest, accommodating for linear (M1-), K48-, and K63-linkage.
    Depositor
    Helle Ulrich
    Available since
    May 31, 2024
    Availability
    Academic Institutions and Nonprofits only
Showing: 6161 - 6200 of 6265 results