We narrowed to 30,515 results for: Ide

1. pLenti-EF1a-SPdCas9-DNMT3B-2A-Blast

   Plasmid

   #71217

   Purpose

   dCas9 fused to human DNMT3B catalytic domain

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-DNMT3B catalytic domain
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-EF1a-SPdCas9-DNMT3A(E752A)-2A-Blast

   Plasmid

   #71218

   Purpose

   dCas9 fused to the human DNMT3A catalytically inactive domain

   Depositor

   Yonglun Luo

   Article

   Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

   Insert

   dSpCas9-DNMT3A(E752A)
   

   Use

   Lentiviral

   Tags

   SPdCas9-DNMT3A(E752A)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDisplay-GRAB_NPYmut

   Plasmid

   #208678

   Purpose

   Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) control sensor GRAB_NPYmut in mammalian cells

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based neuropeptide Y (NPY) control sensor GRAB_NPYmut
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Rat SERCA2a (pMT2)

   Plasmid

   #75183

   Purpose

   Mammalian expression of ATP2A2

   Depositor

   Jonathan Lytton, Gary Shull

   Article

   Gunteski-Hamblin et al J Biol Chem. 1988 Oct 15;263(29):15032-4

   Insert

   ATP2A2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   adenovirus major late promoter

   Available since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pETM33_Orf9b

   Plasmid

   #156485

   Purpose

   Bacterial expression of Sars-CoV2 Orf9b protein with His-tag and GST-tag

   Depositor

   Ylva Ivarsson

   Article

   Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

   Insert

   Orf9b
   

   Use

   Tags

   His-GST

   Expression

   Bacterial

   Mutation

   Gene insert is codon optimized for Ecoli.

   Promoter

   T7/LacO

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-GRAB_NPYmut

   Plasmid

   #208679

   Purpose

   Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) control sensor GRAB_NPYmut in neurons

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based neuropeptide Y (NPY) control sensor GRAB_NPYmut
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-dSpCas9-HF1-VP64-6xHis

   Plasmid

   #92118

   Purpose

   Expression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   dead/inactive SpCas9-HF1-NLS-3xFLAG-VP64
   

   Use

   CRISPR

   Tags

   3xFLAG, 6xHis, NLS, and VP64

   Expression

   Bacterial

   Mutation

   D10A, N497A, R661A, Q695A, H840A, Q926A

   Promoter

   T7

   Available since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. VP16-ER beta (short)

   Plasmid

   #11353

   Depositor

   Donald McDonnell

   Article

   Chang et al Mol Cell Biol. 1999 Dec . 19(12):8226-39

   Insert

   ER beta short (ESR2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 10, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pHS0578 CRISPR associated IscB non-coding locus in pColA-Duet

   Plasmid

   #176535

   Purpose

   Non-protein coding locus components from CRISPR-associated IscB

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

   Insert

   non-coding locus components from Delaware Bay metagenome CRISPR-associated IscB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. PET-SpCas9-HF1-plus-NLS-6xHis

    Plasmid

    #207381

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    SpCas9-HF1-plus
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, Q695A, Q926A, amino acids 1005-1013 replac…

    Promoter

    T7

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. PET-B-SpCas9-HF1-NLS-6xHis

    Plasmid

    #207385

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-SpCas9-HF1
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N497A, R661A, Q695A, Q926A, amino acids 1005-1013…

    Promoter

    T7

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP TEM4 R130D

    Plasmid

    #58895

    Purpose

    Expresses GFP Tagged full length TEM4 protein with R130D mutation. Please see notes below for information regarding the L722F mutation in TEM4.

    Depositor

    Channing Der

    Article

    Mitin et al PLoS One. 2012;7(7):e41876. doi: 10.1371

    Insert

    TEM4 (ARHGEF17 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    R130D, L722F

    Promoter

    CMV

    Available since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCS2-mqgDUX4mqg*

    Plasmid

    #21177

    Depositor

    Stephen Tapscott

    Article

    Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

    Insert

    DUX4 (DUX4 Human)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Point mutation at nucleotide 6163 (numbering acco…

    Promoter

    Available since

    July 22, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pEGFP TEM4 DEL 125-135

    Plasmid

    #58896

    Purpose

    Expresses GFP tagged full length TEM4 protein with amino acids 125 - 135 deleted

    Depositor

    Channing Der

    Article

    Mitin et al PLoS One. 2012;7(7):e41876. doi: 10.1371

    Insert

    TEM4 (ARHGEF17 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deleted aa's 125-135

    Promoter

    CMV

    Available since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pHS0537 CRISPR-associated IscB locus (Delaware Bay) in pBR322 with Fn spacer

    Plasmid

    #176588

    Purpose

    Endogenous locus of CRISPR-associated IscB (Delaware Bay) with Fn spacer

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    CRISPR-associate IscB (Delaware Bay) locus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Replaced spacer in CRISPR array with Fn spacer

    Promoter

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. B-HeFSpCas9

    Plasmid

    #126766

    Purpose

    Expression plasmid for human codon-opt. increased fidelity Blackjack-HeFSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HeFSpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    B-HeFSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A; R661A; Q695A; K848A; Q926A; K1003A; R1060A…

    Promoter

    CBh

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-FLAG-eSpCas9-plus

    Plasmid

    #126774

    Purpose

    Expression of increased fidelity eSpCas9-plus in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with close to the same fidelity as eSpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    eSpCas9-plus
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, MBP, and NLS

    Expression

    Bacterial

    Mutation

    K848A, R1060A; amino acids 1005-1013 replaced wit…

    Promoter

    T7

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. Rat SERCA2b (pMT2)

    Plasmid

    #75184

    Purpose

    Mammalian expression of ATP2A2

    Depositor

    Jonathan Lytton, Gary Shull

    Article

    Gunteski-Hamblin et al J Biol Chem. 1988 Oct 15;263(29):15032-4

    Insert

    ATP2A2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    adenovirus major late promoter

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFRT-TODestFLAGHA_RBPMS

    Plasmid

    #59388

    Purpose

    Destination vector with RBPMS for stable cell line generation

    Depositor

    Thomas Tuschl

    Article

    Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

    Insert

    RBPMS (RBPMS Human)
    

    Use

    Tags

    FLAG HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, 2x Tet

    Available since

    Feb. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28a-FgßF3-ddFLN4-HIS-ybbr

    Plasmid

    #101743

    Purpose

    mutant FgßF3 peptide (additional phenylalanine) with ddFLN4 fingerprint domain with C-terminal ybbr tag for covalent immobilization - resulting in high forces

    Depositor

    Hermann Gaub

    Article

    Milles et al Science. 2018 Mar 30;359(6383):1527-1533

    Insert

    FgßF3 peptide, ddFLN4 fingerprint (FGB Dictyostelium discoideum, Human)
    

    Use

    Tags

    HIS and ybbr

    Expression

    Bacterial

    Mutation

    C18S in ddFLN4, added 9F in FgßF3

    Promoter

    T7

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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