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pL0_DE_mTurquoise2

Plasmid

#202155

Purpose

Level 0 plasmid containing a mTurquoise2 fluorescent protein with DE overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

mTurquoise2 gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_DE-RFP1

Plasmid

#202154

Purpose

Level 0 plasmid containing a RFP1 fluorescent protein with DE overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

RFP1 gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pL0_DE-mRuby3

Plasmid

#202153

Purpose

Level 0 plasmid containing a mRuby3 fluorescent protein with DE overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

mRuby3 gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_DE-3xStop

Plasmid

#202151

Purpose

Level 0 plasmid containing 3 consecutive stop codons with DE overhangs used to build a level 1 constuct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

3x Stop codon

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_CD-eGFP

Plasmid

#202149

Purpose

Level 0 plasmid containing an eGFP fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

eGFP gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pL0_CD-Venus

Plasmid

#202148

Purpose

Level 0 plasmid containing a Venus fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Venus gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_CD-sfGFP

Plasmid

#202147

Purpose

Level 0 plasmid containing a sfGFP fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

sfGFP gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pL0_CD-mTurquoise2

Plasmid

#202146

Purpose

Level 0 plasmid containing a mTurquoise2 fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

mTurquoise2 gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pL0_CD-mRFP1

Plasmid

#202145

Purpose

Level 0 plasmid containing a mRFP1 fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

mRFP1 gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pL0_CD-eYGFP-uv

Plasmid

#202144

Purpose

Level 0 plasmid containing an eYGFP-uv fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

eYGFP-uv gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pL0_CD-mcherry

Plasmid

#202143

Purpose

Level 0 plasmid containing a mCherry fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

mCherry gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pL0_CD-eCFP

Plasmid

#202142

Purpose

Level 0 plasmid containing an eCFP fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

eCFP gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pL0_CD-roGFP

Plasmid

#202140

Purpose

Level 0 plasmid containing a roGFP fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

roGFP gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pL0_CD-eYFP

Plasmid

#202139

Purpose

Level 0 plasmid containing an eYFP fluorescent protein with CD overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

eYFP gene

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pL0_AF-spacer

Plasmid

#202130

Purpose

Level 0 plasmid containing a small non-coding spacer sequence with AF overhangs used to build level 1 constructs.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

Spacer

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 25, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCDNA5/FRT-ChemoR-NAD

Plasmid

#193825

Purpose

CMV overexpression of ChemoR-NAD in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoR-NAD

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoB

Plasmid

#193808

Purpose

CMV overexpression of ChemoB in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoB

Tags

10xHis

Expression

Mammalian

Mutation

EBFP2: N39Y, V206K, T225R; HT7: E143R, E147R, L27…

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT-ChemoY

Plasmid

#193810

Purpose

CMV overexpression of ChemoY in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoY

Tags

10xHis

Expression

Mammalian

Mutation

Venus:A206K, T225R; HT7: E143R, E147R, L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pET-51b(+) ChemoB-CaM

Plasmid

#193812

Purpose

Protein production of ChemoB-CaM in bacteria

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoB-CaM

Tags

10xHis

Expression

Bacterial

Mutation

EBFP2: N39Y, V206K

Promoter

T7

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pCK411.RR1

Plasmid

#192644

Purpose

Expresses sgRNA RR1 (target mRFP CDS) on ColE1-AmpR

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Insert

BBa_J23119-RR1

Use

CRISPR and Synthetic Biology

Promoter

BBa_J23119

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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