We narrowed to 10,054 results for: Uty

1. pET-BoNT/E

   Plasmid

   #168020

   Purpose

   Expresses BoNT/E-LC(1-411)-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Blum et al Science. 2021 Feb 19;371(6531):803-810.

   Insert

   BoNT/E-LC(1-411)-6xHis
   

   Tags

   Hisx6

   Expression

   Bacterial

   Available Since

   Nov. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMK455 (dTAG-mClover-BSD)

   Plasmid

   #214385

   Purpose

   dTAG-mClover for C-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

   Insert

   dTAG-mClover-BSD
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMK456 (dTAG-mCherry-BSD)

   Plasmid

   #214386

   Purpose

   dTAG-mCherry for C-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

   Insert

   dTAG-mCherry-BSD
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSMART-GFPuv

   Plasmid

   #65822

   Purpose

   GFPuv Expression, Low Phosphate Dependent E. coli Expression

   Depositor

   Michael Lynch

   Article

   Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

   Insert

   gfpuv
   

   Expression

   Bacterial

   Promoter

   E . coli insulated waaH gene promoter, low phosp…

   Available Since

   July 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. BbZIP-cALFA

   Plasmid

   #160498

   Purpose

   For the protein expression of BbZIP with ALFA tag at C-terminus in E. coli

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Insert

   BbZIP
   

   Tags

   HRV3C protease cleavage site-ALFA tag-8histidine

   Expression

   Bacterial

   Available Since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. CMV:CD4-HA-CIBN-MKII-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4

   Plasmid

   #135460

   Purpose

   Mammalian expression of uFLARE TF component

   Depositor

   Alice Ting

   Article

   Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

   Insert

   CMV-CD4-HA-CIBN-MKII-NNES-hLOV-TEVcs( ENLYFQM)-GAL4.ape
   

   Tags

   HA, flag,

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-H2GH

   Plasmid

   #182202

   Purpose

   Adeno-associated viral vector to express histone2B-HaloTag-GFP fusion, a nuclear-localized Halotag-GFP fusion construct

   Depositor

   Joshua Kritzer

   Article

   Deprey et al ACS Chem Biol. 2022 Jan 15. doi: 10.1021

   Insert

   histone2B-HaloTag-GFP
   

   Use

   AAV

   Available Since

   May 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. untargeted Plk1 FRET sensor Myt1 substrate

   Plasmid

   #45217

   Depositor

   Michael Lampson

   Article

   Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

   Insert

   untargeted Plk1 FRET sensor Myt1 substrate
   

   Expression

   Mammalian

   Promoter

   t7

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCD5-TS-sfGFP-Tetrabrachion-TEV-N2NL03

   Plasmid

   #158793

   Purpose

   Expresses influenza A virus tetrameric neuraminidase fused to a sfGFP

   Depositor

   Robert de Vries

   Article

   van der Woude et al Protein Sci. 2020 Jul 25. doi: 10.1002/p

   Insert

   Neuraminidase A/NL/109/03
   

   Tags

   TwinStrep_sfGFP_tetrabrachion

   Expression

   Mammalian

   Mutation

   AA61-469

   Promoter

   CMV

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBT29-splitD

    Plasmid

    #122599

    Purpose

    clone M13 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    M13 bacteriophage genes
    

    Expression

    Bacterial

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSGKP_AcsgRNA

    Plasmid

    #203807

    Purpose

    pSGKP-based plasmid containing a J23119-driven sgRNA cassette, with replaceable spacer (BsaI-restriction sites). Kanamycin resistance.

    Depositor

    Quanjiang Ji

    Article

    Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

    Insert

    AcgRNA
    

    Use

    CRISPR

    Promoter

    J23119

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. CENP-B targeted Aurora B FRET sensor Kif2 substrate

    Plasmid

    #45214

    Depositor

    Michael Lampson

    Article

    Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

    Insert

    CENP-B targeted Aurora B FRET sensor
    

    Expression

    Mammalian

    Promoter

    t7

    Available Since

    May 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMK451 (BSD-P2A-dTAG)

    Plasmid

    #214381

    Purpose

    dTAG for N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    BSD-P2A-dTAG
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCK255

    Plasmid

    #171155

    Purpose

    Expression of I-SceI gene for genome engineering in Pseudomonas with sacB gene included for counterselection with sucrose on pBBR1-GmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Insert

    I-SceI_sacB
    

    Expression

    Bacterial

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. APT3-PTEN (PTEN high)

    Plasmid

    #168006

    Purpose

    positive selection PACE Accessory Plasmid

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    PTEN-T3'PAP
    

    Use

    Synthetic Biology

    Available Since

    April 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. T7RNAP Cyslight V174C

    Plasmid

    #205078

    Purpose

    T7RNAP protein expression

    Depositor

    Smita Patel

    Article

    Koh et al Mol Cell. 2018 May 17;70(4):695-706.e5.

    Insert

    T7RNAP Cyslight V174C (T7p07 Bacteriophage T7)
    

    Expression

    Bacterial

    Mutation

    C125S, C347S, C510S, C515S, C530S, C723S V174C

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLJM1-Clover-Prx2-mRuby2

    Plasmid

    #117907

    Purpose

    fluorescent reporter of cytosolic hydrogen peroxide

    Depositor

    Hadley Sikes

    Article

    Langford et al Nat Commun. 2018 Aug 7;9(1):3145. doi: 1

    Insert

    Clover-Prx2-mRuby2
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. APT3-VAMP4

    Plasmid

    #168004

    Purpose

    positive selection PACE Accessory Plasmid

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    VAMP4(I75-L91)-T3'PAP
    

    Use

    Synthetic Biology

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOPS0750

    Plasmid

    #115511

    Purpose

    Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing gusA.

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    gusA pOGG083, T-pharma pOGG003 assembled in pOGG026
    

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Constitutive promoter pNeo pOGG001

    Available Since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. MX01

    Plasmid

    #158624

    Purpose

    GATEWAY and BacMam compatible mammalian expression vector with eGFP expression reporter (eGFP is expressed by same promotor but as a separate polypeptide chain).

    Depositor

    Timm Maier

    Article

    Scaiola et al Sci Adv. 2020 Nov 6;6(45). pii: 6/45/eab

    Type

    Empty backbone

    Use

    Gateway destination vector ; bacmam acceptor vect…

    Tags

    P2A

    Expression

    Mammalian

    Promoter

    CMV-IE

    Available Since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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