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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 3081 - 3100 of 173477 results

  7. SPYtag-GFPnanobody

    Plasmid
    #214837
    Purpose
    This plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.
    Depositor
    Hironori Funabiki
    Article
    Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.
    Insert
    SPYtag-GFPnanobody
    Tags
    His-TEV-SUMO-
    Expression
    Bacterial
    Promoter
    T5
    Available Since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    214837-plasmid-map-sequence-id-425310

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  11. Cys-3HB-SPYcatcher

    Plasmid
    #214838
    Purpose
    This plasmid encodes a protein (Cys-3HB-SPYcatcher) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.
    Depositor
    Hironori Funabiki
    Article
    Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.
    Insert
    Cys-3HB-SPYcatcher
    Tags
    His-TEV-SUMO-
    Expression
    Bacterial
    Promoter
    T5
    Available Since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    214838-plasmid-map-sequence-id-425316

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  16. pCMV-T7-ABE8e-nSpCas9-NG-P2A-EGFP (KAC999)

    Plasmid
    #185914
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized ABE8e-nSpCas9-NG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…
    Promoter
    CMV and T7
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185914-plasmid-map-sequence-id-367363

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  18. pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309)

    Plasmid
    #185913
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available Since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185913-plasmid-map-sequence-id-367365

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Showing: 3081 - 3100 of 173477 results