We narrowed to 5,883 results for: ARL

1. pGL1_9

   Plasmid

   #199041

   Purpose

   Level 1 plasmid, barcoded ORI

   Depositor

   Nili Ostrov, Charlie Gilbert, Cultivarium Tools

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   pSC101ts ORI plus NGS barcode 10
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL1_1

   Plasmid

   #199033

   Purpose

   Level 1 plasmid, barcoded ORI

   Depositor

   Nili Ostrov, Charlie Gilbert, Cultivarium Tools

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   pAL5000 ORI plus NGS barcode 1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL1_47

   Plasmid

   #199054

   Purpose

   Level 1 plasmid, barcoded ORI

   Depositor

   Nili Ostrov, Charlie Gilbert, Cultivarium Tools

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   p15A ORI plus NGS barcode 24
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. isoform PKC Theta (JCE782)

   Plasmid

   #182026

   Purpose

   yeast expression of mouse PKC Theta with yeast PKC1 promoter and ADH1 terminator

   Depositor

   Juan Carlos Igual

   Insert

   PKC Theta (Prkcq Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   yeast PKC1 promoter (-600 to -1)

   Available since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGolt1-mCherry

   Plasmid

   #73361

   Purpose

   In vivo protein protein interaction studies/ expression in mammalian cells

   Depositor

   Michael Kreutz

   Insert

   TMD (trans-membrane domain) of Calneuron-2 (Cabp7 Rat)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV immediate early promoter

   Available since

   Feb. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGolt2-mCherry

   Plasmid

   #73358

   Purpose

   In vivo protein protein interaction studies/ expression in mammalian cells

   Depositor

   Michael Kreutz

   Insert

   TMD (trans-membrane domain) of Calneuron-2 (Cabp7 Rat)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV immediate early promoter

   Available since

   Feb. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX2T E7 del PTLHE

   Plasmid

   #13637

   Depositor

   Karl Munger

   Article

   Wu et al J Virol. 1993 Apr . 67(4):2402-7.

   Insert

   HPV 16 E7 del PTLHE (E7 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   del aa 6-10

   Promoter

   Available since

   March 2, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pComb3XSS

   Plasmid

   #63890

   Purpose

   3rd generation plasmid for phage display on modified geneIII, contains stuffer fragment

   Depositor

   Carlos Barbas

   Article

   Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):

   Inserts

   SS Stuffer
   Light Chain Stuffer
   Heavy Chain Stuffer
   

   Use

   Phage display

   Tags

   6x His, HA tag, and geneIII

   Expression

   Bacterial

   Mutation

   Promoter

   lacZ

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDonor MCS Rosa26

   Plasmid

   #37200

   Depositor

   Charles Gersbach

   Article

   Perez-Pinera et al Nucleic Acids Res. 2012 Apr 1;40(8):3741

   Inserts

   Rosa26 left targeting arm
   MCS
   Rosa26 right targeting arm
   

   Use

   Genomic targeting - zinc finger

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. ph7SK-gRNA

    Plasmid

    #53189

    Purpose

    Expresses the S. pyogenes sgRNA from the 7SK promoter

    Depositor

    Charles Gersbach

    Article

    Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human 7SK

    Available since

    June 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEC3135 (pERASE)

    Plasmid

    #220306

    Purpose

    Enables scarless gene deletions in Streptococcus pyogenes using the counterselection marker pheS*

    Depositor

    Emmanuelle Charpentier

    Article

    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSEED-mGreenLantern:ALFA

    Plasmid

    #219644

    Purpose

    Permits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mScarlet:ALFA using the SEED/Harvest method

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    mGreenLantern:ALFA SEED Cassette
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pY2-MET-IV

    Plasmid

    #218117

    Purpose

    MET-HYGRO plasmid (218133) with the functional cassettes of pY2 (218115) inserted at the sfGFP dropout locus.

    Depositor

    Carl Denard

    Article

    Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pY2-LYS-IV

    Plasmid

    #218118

    Purpose

    LYS-Leu2 plasmid (218114) with the functional cassettes of pY2 (218115) inserted at the sfGFP dropout locus.

    Depositor

    Carl Denard

    Article

    Martinusen et al Protein Eng Des Sel. 2024 Jan 29;37:gzae

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS2FA CO Tol2 TPase

    Plasmid

    #133032

    Purpose

    Expression vector for production of codon optimized Tol2 transposase

    Depositor

    Charles Nathan Hancock

    Article

    Mackey et al MicroPubl Biol. 2020 Jun 11;2020:10.1791

    Insert

    Codon optimized Tol2 Transposase
    

    Use

    In vitro expression

    Tags

    Expression

    Mutation

    Codon Optimized

    Promoter

    Available since

    June 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV[Expression]-mCherry:T2A:Hygro-EF1A>Luc2

    Plasmid

    #174665

    Purpose

    Expresses humanized firefly luciferase and mCherry and Hygromycin resistance linked by T2A, each controlled by a strong promoter

    Depositor

    Eli Bar

    Inserts

    LUC2
    mCherry:T2A:Hygro
    

    Use

    Lentiviral and Luciferase

    Tags

    mCherry and hygromycin dual reporter gene linked …

    Expression

    Mammalian

    Mutation

    Promoter

    Human cytomegalovirus immediate early enhancer/pr…

    Available since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-CalfluxCTN

    Plasmid

    #190068

    Purpose

    Expresses BRET Ca2+ sensor CalfluxCTN in mammalian cells

    Depositor

    Carl Johnson

    Article

    Cumberbatch et al Sci Signal. 2022 Aug 16;15(747):eabq7618

    Insert

    CalfluxCTN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promoter

    Available since

    Sept. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBEVY-U

    Plasmid

    #51230

    Purpose

    bi-directional expression vector for yeast, constitutively active, URA3 selection

    Depositor

    Charles Miller

    Article

    Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPD/ADH1

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-hNox5

    Plasmid

    #69354

    Purpose

    expresses human Nox5 in mammalian cells

    Depositor

    Karl-Heinz Krause

    Article

    Banfi et al J Biol Chem. 2001 Oct 5;276(40):37594-60

    Insert

    hNox5 (NOX5 Human)
    

    Use

    Tags

    no

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5/FRT/TO-TRPT1

    Plasmid

    #184244

    Purpose

    For TRPT1 expression in mammalian cells

    Depositor

    Karla Feijs

    Article

    Weixler et al Nucleic Acids Res. 2022 Sep 9;50(16):942

    Insert

    TRPT1 (TRPT1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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21. pDEST17-hTRPT1-iso1

    Plasmid

    #184239

    Purpose

    For purification of N-terminally His-tagged TRPT1 from bacteria

    Depositor

    Karla Feijs

    Article

    Weixler et al Nucleic Acids Res. 2022 Sep 9;50(16):942

    Insert

    TRPT1 (TRPT1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA

    Plasmid

    #20298

    Purpose

    Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation

    Depositor

    Karl Deisseroth

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    channelrhodopsin-2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    humanized ChR2 gene with histidine 134 changed to…

    Promoter

    EF1alpha

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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23. pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA

    Plasmid

    #20297

    Purpose

    Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation

    Depositor

    Karl Deisseroth

    Has Service

    AAV Retrograde, AAV1, AAV5, AAV8, and AAV9

    Insert

    channelrhodopsin-2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    humanized ChR2 gene with histidine 134 changed to…

    Promoter

    EF1alpha

    Available since

    June 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p102_gRNA_Sa_mClover

    Plasmid

    #213778

    Purpose

    Cloning vector for Sa gRNA, contains Sa gRNA scaffold (with truncations) with mU6 promoter. Includes mClover for bacterial screening and mScarlet for mammalian screening.

    Depositor

    Howard Chang

    Article

    Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    Sa gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    mU6

    Available since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHR-FKBP:mCherry-Rab5a

    Plasmid

    #72901

    Purpose

    Early endosome-targeted mCherry-tagged recruiter construct

    Depositor

    Ivan Yudushkin

    Article

    Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

    Insert

    Rab5a (RAB5A Human)
    

    Use

    Lentiviral

    Tags

    FKBP(F36V) and mCherry

    Expression

    Mammalian

    Mutation

    fragment a.a. 2-215

    Promoter

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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26. pAAV-Ef1a-fDIO-GCaMP6s

    Plasmid

    #105714

    Purpose

    Can be used to express GCaMP6s in the presence of Flp recombinase. Can be used to create adeno-associated virus to express Flp-dependent GCaMP6s.

    Depositor

    Rylan Larsen

    Has Service

    AAV8

    Insert

    GCaMP6s
    

    Use

    AAV; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a, Human elongation factor-1 alpha

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLCS111

    Plasmid

    #209332

    Purpose

    Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter expression of N-terminal mScarlet-I-mNeonGreen tandem fluorescent timer fusion protein.

    Depositor

    Lucia Strader

    Article

    Allen et al Res Sq. 2023 Nov 7:rs.3.rs-3558280. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTargeT gE

    Plasmid

    #60845

    Purpose

    Expression of the varicella zoster glycoprotein (gE) in mammalian cells

    Depositor

    Charles Grose

    Article

    Carpenter et al J Virol. 2011 Sep;85(18):9414-24. doi: 1

    Insert

    ORF68 (ORF68 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

29. pLenti-CaMKIIa-hChR2(C128S)-EYFP-WPRE

    Plasmid

    #20294

    Purpose

    Lentiviral expression of ChR2 (C128S) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

    Depositor

    Karl Deisseroth

    Article

    Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    changed Cysteine 128 to Serine

    Promoter

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1-Cry2FL-VP64

    Plasmid

    #60554

    Purpose

    Expresses Cry2FL-VP64 in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

    Insert

    Cry2FL-VP64
    

    Use

    Tags

    FLAG, HA tag, and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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