We narrowed to 4,850 results for: AAT

1. GOLPH3 shRNA

   Plasmid

   #163860

   Purpose

   shRNA targeting the coding sequences of GOLPH3

   Depositor

   Lei Lu

   Article

   Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27

   Insert

   golgi phosphoprotein 3 (GOLPH3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPBO.ACT005

   Plasmid

   #182715

   Purpose

   Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region

   Depositor

   Joseph Schoeniger

   Article

   Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666

   Insert

   CasRx crRNA cloning backbone
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Catalytically deactivated R295A, H300A, R849A, H8…

   Promoter

   pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)

   Available since

   Aug. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEPQD2KN0908

   Plasmid

   #177088

   Purpose

   MoClo Level 2 plasmid containing multiple transcriptional units for transient expression of P19 (cytosol, driven by nosP), along with CrDXS2, PaGPPS1, and CrGES (plastid, driven by 4x opaatB)

   Depositor

   Nicola Patron

   Article

   Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

   Insert

   P19, CrDXS2, PaGPPS1, and CrGES
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTorPE-G-GECO1.2

   Plasmid

   #32468

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   G-GECO1.2
   

   Use

   Lab constructed

   Tags

   6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

   Expression

   Bacterial

   Mutation

   GCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…

   Promoter

   Available since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. CEBPalpha NGFR

   Plasmid

   #44627

   Depositor

   Ellen Rothenberg

   Article

   Del Real et al Development. 2013 Mar;140(6):1207-19. do

   Insert

   CEBPalpha (Cebpa Mouse)
   

   Use

   Retroviral

   Tags

   IRES and truncated NGFR

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 30, 2013

   Availability

   Academic Institutions and Nonprofits only

6. MAC_C_LMTK2

   Plasmid

   #187752

   Purpose

   MAC-tagged gene expression of human LMTK2

   Depositor

   Markku Varjosalo

   Article

   Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

   Insert

   LMTK2 (LMTK2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiCRISPRv2 sgSp1

   Plasmid

   #174907

   Purpose

   Second generation sgRNA against Sp1

   Depositor

   Jane Clifford

   Article

   Swift et al Cell Rep. 2021 Mar 16;34(11):108840. doi

   Insert

   Specificity Protein 1 (SP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Missense mutation to mutate PAM sequence

   Promoter

   EFS-NS

   Available since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDECKO_TFRC_B

   Plasmid

   #72626

   Purpose

   Expresses two gRNAs targeting the TFRC promoter

   Depositor

   Rory Johnson, Roderic Guigo

   Article

   Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

   Insert

   gRNAs toward TFRC
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (gRNA1) and H1 (gRNA2)

   Available since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. BC1441-mouse U6-3xsgRNAs (TS5, TS6, TS7)

   Plasmid

   #164040

   Purpose

   Expression of three sgRNAs (sgTS5, sgTS6, sgTS7) for targeting to CRISPR-Tag_V2

   Depositor

   Baohui Chen

   Article

   Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

   Insert

   sgTS5-sgTS6-sgTS7
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   mouse U6

   Available since

   Jan. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pgRNA-non-target

    Plasmid

    #129465

    Purpose

    Derived from pSCB2-sgRNA. 20 nucleotide sequence added as sgRNA binding region by inverse PCR. Used as a negative control.

    Depositor

    Silvia Cardona

    Article

    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021

    Insert

    Non-target/random gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 (SpeI)

    Available since

    Sept. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. LEP-shMLL-AF9.1039

    Plasmid

    #105566

    Purpose

    retrovirally express MLL-AF9 shRNA with puro resistance and GFP marker

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    shRNA targeting MLL part of MLL-AF9 fusion
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV-LTR

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. SL2-sgTelo-MTSa/BFP/pdCas9-C1

    Plasmid

    #162760

    Purpose

    Expressing dCas9 and sgRNA containg MTSa targeting telomeres

    Depositor

    Antony K Chen

    Article

    Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

    Insert

    dCas9 and sgRNA(SL2-sgTelo-MTSa)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dCas9(nuclease deactivated Cas9)

    Promoter

    U6/CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1 shTGFBR3 puro

    Plasmid

    #58696

    Purpose

    Lentiviral shRNA vector for knockdown of human TGFBR3

    Depositor

    Kevin Janes

    Article

    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

    Insert

    shTGFBR3 (TGFBR3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

14. LEP-shMLL-AF9.643

    Plasmid

    #105565

    Purpose

    retrovirally express MLL-AF9 shRNA with puro resistance and GFP marker

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    shRNA targeting MLL part of MLL-AF9 fusion
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV-LTR

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pT2SCb

    Plasmid

    #59385

    Purpose

    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a modified chloramphenicol resistance gene.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Insert

    TT-ISceI-cat2
    

    Use

    Template

    Tags

    Expression

    Mutation

    The 3’-end of the chloramphenicol resistance gene…

    Promoter

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBLO2 casX

    Plasmid

    #123122

    Purpose

    E. coli expression vector for CasX sgRNA

    Depositor

    Benjamin Oakes, Jennifer Doudna

    Article

    Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

    Insert

    CasX guide RNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEPQD2KN0897

    Plasmid

    #177090

    Purpose

    MoClo Level 2 plasmid containing multiple transcriptional units for transient expression of CrISY, NmMLPL, Cr7-DLGT, CrGOR, CrIO, and CrG8H driven by 4x opaatB

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    CrISY, NmMLPL, Cr7-DLGT, CrGOR, CrIO, and CrG8H
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-TO-HA-SH3BP1 (RNAi-res)

    Plasmid

    #136645

    Purpose

    Expression of SH3BP1 in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUC

    Depositor

    Karl Matter, Maria Balda

    Article

    Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

    Insert

    SH3BP1 (RNAi resistant) (SH3BP1 Human)
    

    Use

    Tags

    HA (MQDLPGNDNSTAG)

    Expression

    Mammalian

    Mutation

    GATGACAGCCACCCACTTC changed to AATGACAGCAACACACTTC

    Promoter

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. SL2-sgTelo-MTSa/EGFP/pdCas9-C1

    Plasmid

    #162759

    Purpose

    Expressing dCas9 and sgRNA containg MTSa targeting telomeres

    Depositor

    Antony K Chen

    Article

    Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

    Insert

    dCas9 and sgRNA(SL2-sgTelo-MTSa)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    dCas9(nuclease deactivated Cas9)

    Promoter

    U6/CMV

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMKO.1-Zfp423

    Plasmid

    #35972

    Depositor

    Bruce Spiegelman

    Article

    Gupta et al Nature. 2010 Mar 25;464(7288):619-23. do

    Insert

    zfp423 shRNA (Zfp423 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2012

    Availability

    Academic Institutions and Nonprofits only