We narrowed to 13,220 results for: CRISPR-Cas9

1. pX330_SpCas9_USP7 Exon 3 gRNA

   Plasmid

   #131257

   Purpose

   pX330-U6-Chimeric_BB-Cbh-hSpCas9 vector expressing a guide RNA targeting exon 3 of USP7

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

   Insert

   humanised S. pyogenes Cas9 nuclease
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENTR-L3-Cas9n-EGFP-L2

   Plasmid

   #141044

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   Cas9n-2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR-L3-Csy4-Cas9n-L2

   Plasmid

   #141041

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   Csy4-2A-Cas9n
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENTR-L3-Cas9-EGFP-L2

   Plasmid

   #141043

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   Cas9-2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR-L3-Csy4-Cas9-L2

   Plasmid

   #141040

   Purpose

   Mutisite Gateway mediated vector construction

   Depositor

   Hideyuki Okano

   Article

   Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

   Insert

   Csy4-2A-Cas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL3-sgSEC61B.N1-CAG-Cas9-T2A-mCherry-P2A-Puro

   Plasmid

   #216251

   Purpose

   Express Cas9 with CAG promoter to improve the expression in hES cells and sgSEC61B.N1 to tag endogenous SEC61B N-terminus

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   Cas9 and sgSEC61B.N1 (SEC61B Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL3-sgRANGAP.C1-CAG-Cas9-T2A-mCherry-P2A-Puro

   Plasmid

   #216249

   Purpose

   Express Cas9 with CAG promoter to improve the expression in hES cells and sgRANGAP.C1 to tag endogenous RANGAP1 C-terminus

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   Cas9 and sgRANGAP.C1 (RANGAP1 Human)
   

   Use

   CRISPR; Endogenous tagging

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. Polymerase-recruited click editor - pCMV-T7-PCV2-nCas9-CC(N6) (LM2705)

   Plasmid

   #208962

   Purpose

   Polymerase-recruited click editor comprised of PCV2 fused to nCas9 with a C-terminal N6 coiled coil (CC) domain, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-CC(N6)
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A)

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. Control click editor - pCMV-T7-PCV2-nCas9-deadEcKlenow (JO520)

   Plasmid

   #208945

   Purpose

   A control CE1 construct with catalytically attenuated EcKlenow DNA polymerase, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-dEcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A);deadEcKlenow(-exo;D355A,D357A,D705…

   Promoter

   CMV and T7

   Available since

   Nov. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. Cas9_ANKRD1_sgRNA2

    Plasmid

    #186669

    Purpose

    Plasmid containing Cas9 and ANKRD1 sgRNA2 , sgRNA sequence: cggtcagcttatatagct.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    ANKRD1 KO sgRNA Plasmid (ANKRD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-TRE-dCas9-VPR-pA-3xsgRNA-EF1a-Tet.O-T2A-PuroR-polyA

    Plasmid

    #166693

    Purpose

    Express dCas9-VPR in a doxycyclin-inducable system and 3 sgRNAs targeting the murine Cnga1 promoter. Can be stably integrated into the genome via the PiggyBac Transposon system.

    Depositor

    Elvir Becirovic

    Article

    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

    Inserts

    dCas9-VPR
    3x Cnga1 promoter-targeting sgRNAs
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and U6

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR-UCOE-EF1a-Zim3-dCas9-loxP-P2A-EGFP-loxP

    Plasmid

    #188773

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHR-UCOE-SFFV-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP

    Plasmid

    #188765

    Purpose

    dCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    dCas9-XTEN80-KRAB(Kox1)
    

    Use

    Lentiviral

    Tags

    HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHR-UCOE-CMV-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP

    Plasmid

    #188772

    Purpose

    dCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    dCas9-XTEN80-KRAB(Kox1)
    

    Use

    Lentiviral

    Tags

    HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR-UCOE-SFFV-Zim3-dCas9-loxP-P2A-EGFP-loxP

    Plasmid

    #188774

    Purpose

    dCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLS

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Zim3-dCas9
    

    Use

    Lentiviral

    Tags

    HA-2xNLS and Zim3 KRAB-NLS fusion

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Oct. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pHR-UCOE-EFS-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP

    Plasmid

    #188771

    Purpose

    dCas9 with a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    dCas9-XTEN80-KRAB(Kox1)
    

    Use

    Lentiviral

    Tags

    HA-2xNLS-XTEN80(linker)-KRAB(Kox1)

    Expression

    Mutation

    Promoter

    EFS

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR GLIS3 TSS-guide3

    Plasmid

    #118175

    Purpose

    CRISPR-mediated repression of GLIS3. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2 TSS-guide1

    Plasmid

    #118169

    Purpose

    CRISPR-mediated repression of TCF7L2. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2 TSS-guide2

    Plasmid

    #118170

    Purpose

    CRISPR-mediated repression of TCF7L2. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR CRY2-TSS-guide1

    Plasmid

    #125428

    Purpose

    CRISPR-mediated repression of CRY2. KRAB domain and Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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