We narrowed to 11,279 results for: Vars

1. CP1300-ABEmax

   Plasmid

   #119810

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

   Insert

   ecTadA-ecTadA*(7.10)-nSpCas9 (CP1300)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-STAT3-linker-TagGFP2

   Plasmid

   #121427

   Purpose

   WT STAT3 inserted using Infusion Cloning into MluI and EcoRI digested pLenti-Origene-Nrf21.

   Depositor

   Ovijit Chaudhuri, Stanley Qi

   Article

   Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

   Insert

   STAT3-linker-TagGFP2
   

   Use

   Tags

   TagGFP2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. CMV+ plasmid with onboard mCherry cassette

   Plasmid

   #169741

   Purpose

   CMV+ plasmid that also encodes a separate mCherry cassette. mCherry is used to monitor plasmid dosage.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV-tetO2

   Available since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-sCS4-2T

   Plasmid

   #28250

   Depositor

   Yawen Bai

   Article

   Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

   Insert

   single chain Cse4-Scm3-H4 with 2 Thrombin site
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   A thrombin cutting site between Cse4 and Scm3. An…

   Promoter

   Available since

   July 12, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pAcGFP1-N1-CAHS3

   Plasmid

   #90031

   Purpose

   To express GFP-tagged CAHS3 in mammalian cells transiently

   Depositor

   Takekazu Kunieda

   Article

   Yamaguchi et al PLoS One. 2012;7(8):e44209.

   Insert

   CAHS3
   

   Use

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCK671.306

   Plasmid

   #192641

   Purpose

   Expresses dxCas9-NG and MCP-SoxS(R93A/S101A) and scRNA J306 on p15A-CmR

   Depositor

   Jesse Zalatan

   Article

   Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

   Insert

   J306 scRNA
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   BBa_J23119

   Available since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-mSmo-WT-V5

   Plasmid

   #126415

   Purpose

   Localization, Expression of Smoothened WT in mammalian cell lines.

   Depositor

   Stacey Ogden

   Article

   Marada et al PLoS Genet. 2015 Aug 20;11(8):e1005473.

   Insert

   m-Smoothened (Smo Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. Plas-gRNA-LEU2

   Plasmid

   #157656

   Purpose

   GFP and gRNA tag for protein and mRNA quantification, to attache to the 3' end of the gene coding sequence

   Depositor

   Frank Albert

   Article

   Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

   Insert

   GFP-gRNA
   

   Use

   Insert storage (replicative in e. coli)

   Tags

   GFP-gRNA

   Expression

   Mutation

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-DUET-1-mRhubarb719-HO

   Plasmid

   #141200

   Purpose

   Expresses mRhubarb719 fluorescent protein and heme oxygenase

   Depositor

   Elad Firnberg

   Article

   Rogers et al Sci Rep. 2019 Oct 30;9(1):15653. doi: 10

   Inserts

   mRhubarb719
   heme oxygenase
   

   Use

   Tags

   6x-His

   Expression

   Bacterial

   Mutation

   R134H, Y198S, T202Y, V203I, W309R, Q310A compared…

   Promoter

   T7

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQP-2460

    Plasmid

    #138973

    Purpose

    Light-induced recruitment of QPAS1-mCherry to the target protein bound to membrane; interaction occurs via intrabody fused to BphP1

    Depositor

    Vladislav Verkhusha

    Article

    Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

    Insert

    NcoI-mVenus-CAAX-IRES2-BphP1- iB(GFP)- IRES2-NES-mCherry-Q-PAS1-NotI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDH3

    Plasmid

    #83789

    Purpose

    Useful for tagging yeast proteins with C-terminal CFP tag; G418 selection

    Depositor

    Eric Muller

    Article

    Hailey et al Methods Enzymol. 2002;351:34-49.

    Insert

    CFP
    

    Use

    Tags

    CFP

    Expression

    Yeast

    Mutation

    F64L, Y66W, N146I, M153T, V163A, N164H mutations …

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBSc + MShab

    Plasmid

    #16146

    Depositor

    Larry Salkoff

    Article

    Pak et al J Neurosci. 1991 Mar . 11(3):869-80.

    Insert

    Mus musculus potassium voltage gated channel (Kcnb1 Mouse, Fly)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pAcGFP1-N1-CAHS1

    Plasmid

    #90030

    Purpose

    To express GFP-tagged CAHS1 in mammalian cells transiently

    Depositor

    Takekazu Kunieda

    Article

    Yamaguchi et al PLoS One. 2012;7(8):e44209.

    Insert

    CAHS1
    

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBSc polyA + Shal1

    Plasmid

    #16196

    Depositor

    Larry Salkoff

    Article

    Wei et al Science. 1990 May 4. 248(4955):599-603.

    Insert

    Drosophila melanogaster Shaker cognate l (Shal Fly)
    

    Use

    Xenopus oocyte expression

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

15. GTT-g

    Plasmid

    #81109

    Purpose

    Gene Tagging vector TRP/GFP - Plasmid for superfolder GFP (sfGFP) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Insert

    sfGFP
    

    Use

    Cre/Lox

    Tags

    sfGFP

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. Pinco-mNrp1dCy

    Plasmid

    #21936

    Depositor

    Guido Serini

    Article

    Valdembri et al PLoS Biol. 2009 Jan 27. 7(1):e25.

    Insert

    neuropilin 1 (Nrp1 Mouse)
    

    Use

    Retroviral

    Tags

    HA and His

    Expression

    Mutation

    deleted amino acids 884-923

    Promoter

    Available since

    Nov. 10, 2009

    Availability

    Academic Institutions and Nonprofits only

17. Pinco-mNrp1dSEA

    Plasmid

    #21935

    Depositor

    Guido Serini

    Article

    Valdembri et al PLoS Biol. 2009 Jan 27. 7(1):e25.

    Insert

    neuropilin 1 (Nrp1 Mouse)
    

    Use

    Retroviral

    Tags

    HA and His

    Expression

    Mutation

    deleted amino acids 920-923

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

18. GTH-r

    Plasmid

    #81106

    Purpose

    Gene Tagging vector HIS/RFP - Plasmid for monomeric Cherry (mCherry) labeling of endogenous proteins with a HIS selection marker, originating from pSIVh (ID:81091)

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Insert

    mCherry
    

    Use

    Cre/Lox

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCFJ2259 - Intron GG2 - 900 bp

    Plasmid

    #159881

    Purpose

    Golden Gate compatible intron with PATCs for reduced germline silencing

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    Intron GG2 - 900 bp
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSopB2xHA

    Plasmid

    #112513

    Purpose

    PsopB driven sopB-2xHA and sigE

    Depositor

    Olivia Steele-Mortimer

    Article

    Knodler et al Cell Microbiol. 2009 Nov;11(11):1652-70.

    Insert

    sopB-2xHA/sigE operon
    

    Use

    Tags

    2xHA

    Expression

    Bacterial

    Mutation

    SopB C-terminal 2xHA

    Promoter

    PsopB

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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