We narrowed to 4,230 results for: erf

1. pHAGE-TO-dCas9-P2A-HSA

   Plasmid

   #121936

   Purpose

   CRISPR-Sirius plasmid

   Depositor

   Thoru Pederson

   Article

   Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

   Insert

   dCas9-P2A-HSA
   

   Use

   CRISPR and Lentiviral

   Tags

   HSA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TO

   Available since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET30-T7-His-sfGFP

   Plasmid

   #180755

   Purpose

   pET30 vector missing lacO with sfGFP-S-tag inserted between the Bgl II and T7 terminator

   Depositor

   Michael Black

   Article

   Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

   Insert

   superfolder green fluorescent protein
   

   Use

   Tags

   strep tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAbaMCi-BsaI_pJMP2776

   Plasmid

   #208890

   Purpose

   Acinetobacter baumannii CRISPRi plasmid for cloning new guides (BsaI cloning site in gRNA cassette).

   Depositor

   Jason Peters

   Article

   Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

   Insert

   dCas9, sgRNA expression cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMM704

   Plasmid

   #68891

   Purpose

   IPTG-inducible CRISPRi vector targeting BT1854, pNBU2 backbone, AmpR

   Depositor

   Christopher Voigt, Timothy Lu

   Article

   Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

   Inserts

   dCas9
   LacI
   sgRNA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAFi-dHdrA

   Plasmid

   #184907

   Purpose

   carrying CRISPR/dCas9 system for gene knockdown in Acidithiobacillus ferridurans

   Depositor

   Radhakrishnan Mahadevan

   Article

   Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

   Insert

   Cm-sgRNA-dCas9
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. LMAPCW

   Plasmid

   #205655

   Purpose

   shRNAmir backbone for RNA interference, with mCherry reporter

   Depositor

   Dapeng Wang

   Article

   Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

   Insert

   miR-AB
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   mCMV

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMP71-IFN-B

   Plasmid

   #174597

   Purpose

   Expresses secreted murine interferon beta

   Depositor

   Stanley Riddell

   Article

   Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

   Insert

   secreted IFN-beta
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-RILP

   Plasmid

   #74600

   Purpose

   used for expression in E. coli and purification

   Depositor

   Ron Vale

   Article

   Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

   Insert

   Rab-interacting lysosomal protein (RILP Human)
   

   Use

   Tags

   FLAG, StrepII, and superfolder GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   June 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWS5390-CRISPRai-URA3

   Plasmid

   #182829

   Purpose

   Saccharomyces cerevisiae inducible CRISPRai vector - URA3 marker

   Depositor

   Rodrigo Ledesma-Amaro

   Article

   Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSET-dCas9-actII-4-NT-S1

    Plasmid

    #110185

    Purpose

    Repression of gene expression in Streptomyces by CRISPRi

    Depositor

    Yinhua Lu

    Article

    Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

    Inserts

    dCas9
    sgRNA
    

    Use

    CRISPR; I

    Tags

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    J23119 and ermE*p

    Available since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pWS5398-CRISPRai-HygR

    Plasmid

    #182837

    Purpose

    Saccharomyces cerevisiae inducible CRISPRai vector - HygR marker

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMB1-A-ptet-dCas9

    Plasmid

    #190129

    Purpose

    Level 1 MoClo plasmid containing an aTC inducible dCas9 for CRISPRi

    Depositor

    Veronica Godoy-Carter

    Article

    Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pgRNA-R-gfp

    Plasmid

    #220927

    Purpose

    Expresses gfp using J23119 promoter and pgRNA_AT backbone

    Depositor

    Andrea Puhar

    Article

    Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

    Insert

    superfolder GFP
    

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    J23119

    Available since

    Aug. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. LMAPPW

    Plasmid

    #205657

    Purpose

    shRNAmir backbone for RNA interference, with Puromycin selection

    Depositor

    Dapeng Wang

    Article

    Wang et al EMBO Rep. 2022 Apr 5;23(4):e53691. doi:

    Insert

    miR-AB
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    mCMV

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. DT035A

    Plasmid

    #159397

    Purpose

    Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli tac promoter

    Depositor

    Sheref Mansy

    Article

    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

    Insert

    pr.tac-sfGFP
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pWS5397-CRISPRai-NatR

    Plasmid

    #182836

    Purpose

    Saccharomyces cerevisiae inducible CRISPRai vector - NatR marker

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYR69 (X-4:4xUAS-SSA1p-365:yEGFP3-CYC1t)

    Plasmid

    #167355

    Purpose

    Integrative plasmid for promoter-reporter 4xUAS-SSA1p-365:yEGFP3-CYC1t

    Depositor

    Michael Jensen

    Article

    Romero-Suarez et al ACS Synth Biol. 2021 Feb 12. doi: 10.102

    Insert

    4xUAS-SSA1p-365:yEGFP3-CYC1t
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMB1-A-pBAD-dCas9

    Plasmid

    #190132

    Purpose

    Level 1 MoClo plasmid containing an arabinose inducible dCas9 for CRISPRi

    Depositor

    Veronica Godoy-Carter

    Article

    Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

    Insert

    dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX330A_dCas9-1x2

    Plasmid

    #63596

    Purpose

    Expresses dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    humanized S. pyogenes dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D1361Y

    Promoter

    CBh

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pWS5396-CRISPRai-KanR

    Plasmid

    #182835

    Purpose

    Saccharomyces cerevisiae inducible CRISPRai vector - KanR marker

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge