We narrowed to 4,502 results for: erf
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Pooled Library#161829PurposeInducible genome-wide CRISPRi library targeting S. cerevisiae genes with 6-12 guide RNAs per gene.DepositorExpressionYeastSpeciesSaccharomyces cerevisiaeUseCRISPRAvailable SinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only
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pExp-sfGFP
Plasmid#112573PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and sfGFP (superfolder green fluorescent protein) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneTags8xHis, Strep II, and sfGFPExpressionBacterialAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-dCas9-KRAB_Hygro
Plasmid#192663Purpose3rd generation lenti vector encoding dCas9-KRAB with 2A Hygro resistance markerDepositorInsertdCas9-KRAB
UseCRISPR and LentiviralExpressionMammalianMutationD10A, D839A, H840A and N863A in Cas9PromoterEF1aAvailable SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR Ef1a-dCas9-BFP-KRAB
Plasmid#217304Purposelentiviral vector for dCas9-BFP-KRAB on Ef1alpha promoterDepositorInsertdCas9-tagBFP-KRAB
UseCRISPR, Lentiviral, and Synthetic BiologyPromoterEf1alphaAvailable SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTH385-GST-TEV-SUP35
Plasmid#29744DepositorInsertSUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
TagsGSTExpressionBacterialMutationwild typeAvailable SinceAug. 8, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTH278-GST-TEV-SUP45
Plasmid#29743DepositorInsertSUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
TagsGSTExpressionBacterialMutationwild typeAvailable SinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRL-TK CXCR4 1P
Plasmid#11309DepositorInsert1 perfect bind site for CXCR4 siRNA antisense
UseLuciferaseTagsRr-lucAvailable SinceFeb. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-R-3xSV40pA-R-td-sfGFP
Plasmid#177150PurposeCAG tdGFP dre/rox reporter piggyBac transposon (3x SV40pA stop)DepositorInsertTandem dimer superfolder GFP
UseDre / rox ; piggybacExpressionMammalianPromoterCAGAvailable SinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3 CXCR4 1P
Plasmid#11310DepositorInsert1 perfect binding site for CXCR4 siRNA antisense
UseLuciferaseTagsPp-lucAvailable SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
CAG-loxP-3xpA-H2BsfGFP-tdTomato - opt GS PQR Var3 P2A -APGST E
Plasmid#113847PurposeHistone H2B-superfolder GFP and tdTomato linked by P2A (-APGST linker)DepositorInsertH2B-sfGFP-P2A-tdTomato
ExpressionMammalianAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3 CXCR4 2P
Plasmid#11311DepositorInsert2 perfect binding sites for CXCR4 siRNA antisense
UseLuciferaseTagsPp-lucAvailable SinceFeb. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
U6+27-16pf
Plasmid#21976DepositorInsertU6 miR-16 perfect
ExpressionMammalianAvailable SinceNov. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTH340-SUP45(1-242)-GalBD
Plasmid#29740DepositorInsertSUP45 (SUP45 Budding Yeast)
TagsGal Activation domain and MycExpressionYeastMutationCodons 1-242 of the yeast SUP45 gene onlyAvailable SinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGL3 CXCR4 3P
Plasmid#11312DepositorInsert3 perfect binding sites for CXCR4 siRNA antisense
UseLuciferaseTagsPp-lucAvailable SinceFeb. 15, 2006AvailabilityAcademic Institutions and Nonprofits only -
PB CAG-R-bpA-3xSV40pA-R-td-sfGFP
Plasmid#133579PurposeCAG tdGFP dre/rox reporter piggyBac transposon (bpA 3x SV40pA stop)DepositorInsertTandem dimer superfolder GFP
UseDre/roxExpressionMammalianAvailable SinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
C105m
Plasmid#121008PurposeMoClo golden gate assembly CD part for sfYFP-AAV (superfolder YFP with AAV degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.DepositorInsertsfYFP-AAV ssrA (has bbsI)
UseSynthetic BiologyTagsAAV degradation tag for ssrAAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C106m
Plasmid#121009PurposeMoClo golden gate assembly CD part for sfYFP-DAS (superfolder YFP with DAS degradation tag for ssrA). Please see Supplemental Documents for annotated Genbank file.DepositorInsertsfYFP-DAS ssrA (has bbsI)
UseSynthetic BiologyTagsDAS degradation tag for ssrAAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTH339-SUP45(1-138)-GalBD
Plasmid#29739DepositorInsertSUP45 (SUP45 Budding Yeast)
TagsGal Activation domain and MycExpressionYeastMutationCodons 1-138 of the yeast SUP45 gene onlyAvailable SinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
U6+27-20pf
Plasmid#21978DepositorInsertU6 miR-20 perfect
ExpressionMammalianAvailable SinceNov. 12, 2009AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-dCas9-KRAB-MeCP2_Hygro
Plasmid#192664Purpose3rd generation lenti vector encoding dCas9-KRAB-MeCP2 with 2A Hygro resistance markerDepositorInsertdCas9-KRAB-MeCP2
UseCRISPR and LentiviralExpressionMammalianMutationD10A, D839A, H840A and N863A in Cas9PromoterEF1aAvailable SinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only