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pLpC-L-IFP-F[1] (mammalian)

Plasmid

#52901

Purpose

Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[1] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[1]

Use

Retroviral

Expression

Mammalian

Promoter

Unknown

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
pLpC-L-IFP-F[2] (mammalian)

Plasmid

#52902

Purpose

Binary retroviral pl. (puromycin). MCS:(GGGGS)X 2 + IFP-F[2] (ClaI/EcoRI). Insert can be subcloned between the MCS. Plasmid confers ampicillin resistance to DH5α.

Depositor

Stephen Michnick

Article

Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:

Insert

L-IFP-F[2]

Use

Retroviral

Expression

Mammalian

Promoter

Unknown

Available Since

June 5, 2014

Availability

Academic Institutions and Nonprofits only
pAAV_TALEN-FokI(WT)-BB_V3

Plasmid

#42588

Depositor

Feng Zhang

Article

Cong et al Nat Commun. 2012 Jul 24;3:968. doi: 10.1

Insert

TALEN-FokI(WT)-BB

Use

AAV; Adeno-associated virus ()

Tags

3XFlag

Expression

Mammalian

Promoter

CAG hybrid

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only

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pGL2B -376/+64

Plasmid

#24945

Depositor

Stephen Smale

Article

Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

Insert

IL-10 promoter (Il10 Mouse)

Use

Luciferase

Tags

Luciferase

Available Since

Aug. 18, 2010

Availability

Academic Institutions and Nonprofits only
pCFJ1656

Plasmid

#51483

Purpose

miniMos transposable element with HygroR and peel-1, R4-R3 Gateway cloning

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Type

Empty backbone

Expression

Worm

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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pGL2B -688/+64

Plasmid

#24944

Depositor

Stephen Smale

Article

Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

Insert

IL-10 promoter (Il10 Mouse)

Use

Luciferase

Tags

Luciferase

Available Since

Aug. 18, 2010

Availability

Academic Institutions and Nonprofits only
pGL2B -78/+64

Plasmid

#24947

Depositor

Stephen Smale

Article

Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

Insert

IL-10 promoter (Il10 Mouse)

Use

Luciferase

Tags

Luciferase

Available Since

Aug. 18, 2010

Availability

Academic Institutions and Nonprofits only
pHPdestVenus

Plasmid

#24568

Depositor

Ingrid Lohmann

Article

Boy et al Genesis. 2010 Apr 22. [Epub ahead of pri

Insert

Use

Reporter construct

Tags

Venus

Available Since

May 19, 2010

Availability

Academic Institutions and Nonprofits only

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pHJ346

Plasmid

#20678

Depositor

Stephen Shaw

Article

Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

Insert

Moesin (MSN Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed Lys 253, 254, 262, 263 to Asn

Available Since

May 28, 2009

Availability

Academic Institutions and Nonprofits only
pDest472 SHP-1 S591D

Plasmid

#21806

Depositor

Stephen Shaw

Article

Liu et al J Leukoc Biol. 2007 Sep . 82(3):742-51.

Insert

SHP-1 (PTPN6 Human)

Tags

GFP

Expression

Mammalian

Mutation

changed Serine 591 to Aspartic Acid

Available Since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only
pDEST-IRES-puro-polyA

Plasmid

#43967

Depositor

Peter Hohenstein

Article

Dolt et al PLoS One. 2013 Apr 29;8(4):e62054. doi:

Insert

puroR

Use

Gateway dest vector

Available Since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only

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pCFJ1258

Plasmid

#44478

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Insert

Minimal Mos1, [4-3] Gateway

Use

Gateway destination vector

Available Since

April 25, 2013

Availability

Academic Institutions and Nonprofits only

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pDest472 SHP-1 S591A

Plasmid

#21805

Depositor

Stephen Shaw

Article

Liu et al J Leukoc Biol. 2007 Sep . 82(3):742-51.

Insert

SHP-1 (PTPN6 Human)

Tags

GFP

Expression

Mammalian

Mutation

Serine 591 changed to Alanine

Available Since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only
pFS337-USP44-FLAG

Plasmid

#43905

Depositor

Stephen Elledge

Article

Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

Insert

USP44 (USP44 Human)

Use

Baculoviral expression

Tags

FLAG

Expression

Insect

Available Since

April 1, 2013

Availability

Academic Institutions and Nonprofits only
pAC-EsaR-I70V

Plasmid

#47663

Purpose

pAC-EsaR with a208g mutation in esaR

Depositor

Cynthia Collins

Article

Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

Insert

esaR-I70V

Use

Synthetic Biology

Expression

Bacterial

Mutation

Changed Isoleucine 70 to Valine

Promoter

Plac

Available Since

Sept. 6, 2013

Availability

Academic Institutions and Nonprofits only
pCS2-USP44

Plasmid

#43893

Depositor

Stephen Elledge

Article

Stegmeier et al Nature. 2007 Apr 19;446(7138):876-81.

Insert

USP44 (USP44 Human)

Expression

Mammalian

Promoter

SP6 or T7

Available Since

Aug. 15, 2013

Availability

Academic Institutions and Nonprofits only
pFBXC3H

Plasmid

#49050

Purpose

FX cloning insect cell expression vector

Depositor

Markus Gruetter

Article

Weinert et al Sci Rep. 2015 Jun 4;5:10819. doi: 10.103

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Insect

Promoter

polH (polyhedrin)

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pFBXNH3

Plasmid

#49049

Purpose

FX cloning insect cell expression vector

Depositor

Markus Gruetter

Article

Weinert et al Sci Rep. 2015 Jun 4;5:10819. doi: 10.103

Type

Empty backbone

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Insect

Promoter

polH (polyhedrin)

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pGL2B -55/+64

Plasmid

#24948

Depositor

Stephen Smale

Article

Brightbill et al J Immunol. 2000 Feb 15. 164(4):1940-51.

Insert

IL-10 promoter (Il10 Mouse)

Use

Luciferase

Tags

Luciferase

Available Since

June 4, 2010

Availability

Academic Institutions and Nonprofits only
pHJ386

Plasmid

#20679

Depositor

Stephen Shaw

Article

Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

Insert

Moesin (MSN Human)

Tags

GFP

Expression

Mammalian

Mutation

Changed Lys 253, 254, 262, 263 to Asn; Changed Th…

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only