We narrowed to 2,346 results for: puc19

1. pCS019

   Plasmid

   #207913

   Purpose

   Level 0 for gRNA in D1 module (5' TCAG-3' TGAC)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   pU6-AarI-scaffold-term
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. NK621

   Plasmid

   #196405

   Purpose

   Strong expression of firefly luciferase codon-optimized for S. rosetta

   Depositor

   Nicole King

   Article

   Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

   Insert

   Sro5actin-firefly-Sro3actin
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   N/A (synthetic)

   Promoter

   Available since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. NK809

   Plasmid

   #196406

   Purpose

   Strong expression of firefly and nanoluc luciferases codon-optimized for S. rosetta

   Depositor

   Nicole King

   Article

   Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

   Insert

   Sro5EFL-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   N/A (synthetic)

   Promoter

   Available since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. NK810

   Plasmid

   #196407

   Purpose

   Nanoluc luciferase under S. rosetta SPAG6 promoter with internal actin-firefly control

   Depositor

   Nicole King

   Article

   Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

   Insert

   Sro5spag6-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   N/A (synthetic)

   Promoter

   Available since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCAG-AaCas12b-T2A-EGFP

   Plasmid

   #188271

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Chen et al Innovation (Camb). 2022 May 26;3(4):1002

   Insert

   AaCas12b
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CAG

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG-PlmCasX-T2A-EGFP

   Plasmid

   #188273

   Purpose

   Mammalian expression, Genome editing

   Depositor

   Wei Li

   Article

   Chen et al Innovation (Camb). 2022 May 26;3(4):1002

   Insert

   PlmCasX
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CAG

   Available since

   July 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAcrVIB1-cloDF-amp

   Plasmid

   #184843

   Purpose

   Expression of anti-CRISPR protein AcrVIB1 in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   anti-CRISPR protein inhibiting Cas13b nucleases
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pILGFP4D42

   Plasmid

   #185840

   Purpose

   Testing the TEF1 promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter

   Depositor

   Claudia Vickers

   Article

   Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

   Insert

   PURA3>KlURA3>TAgTEF1-PTEF1+[Z268]>yEGFP>TPGK1-TURA3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIALD2S

   Plasmid

   #185865

   Purpose

   Knocking out ALD6 and expressing anacetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP ) in Saccharomyces cerevisiae

   Depositor

   Claudia Vickers

   Article

   Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

   Insert

   ALD6 (-125, 40)> PADH1> Lr.pduP> TPDC1-ALD6 (1054, 1749)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pILGFP10CF71

    Plasmid

    #185860

    Purpose

    Testing the SkGAL2 promoter inserted with 4 Z268 elements using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1- PSkGAL2+[Z268]>(BamHI)yEGFP>TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge