We narrowed to 15,106 results for: ENA

1. pSLiP-G2-RacE

   Plasmid

   #188966

   Purpose

   L-Rhamnose inducible catalytically inactive Cas9 with sgRNA and racE gene for bacterial gene knockdown

   Depositor

   Seung-Goo Lee

   Article

   Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

   Inserts

   dCas9
   sgRNA: gttagacgctgattacatggactagg
   racE
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PrhaBAD

   Available since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRvCAHS1-mEGFP-NLS

   Plasmid

   #205013

   Purpose

   The vector contains 1kbp of the upstream and downstream regions of the cahs1 gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.

   Depositor

   Kazuharu Arakawa

   Article

   Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

   Insert

   mEGFP-NLS flanked by 1kbp upstream and downstream of the cahs1 gene from Ramazzottius varieornatus
   

   Use

   Tardigrade expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRvSAHS1-mCherry-NLS

   Plasmid

   #205009

   Purpose

   The vector contains 1kbp of the upstream and downstream regions of the sahs1 gene from Ramazzottius varieornatus, with the mCherry gene with NLS sequence positioned in between.

   Depositor

   Kazuharu Arakawa

   Article

   Tanaka et al Proc Natl Acad Sci U S A. 2023 Jan 31;12

   Insert

   mCherry-NLS flanked by 1kbp upstream and downstream of the sahs1 gene from R. varieornatus
   

   Use

   Tardigrade expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. DCT.VV.Orf42

   Plasmid

   #12730

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jon Yewdell, Jack Bennink

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf42
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 1, 2006

   Availability

   Academic Institutions and Nonprofits only

5. LentiCRISPR v2-sgAMPK alpha1 clone2

   Plasmid

   #162120

   Purpose

   Lentiviral sgRNA plasmid targeting human AMPK alpha1

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgAMPK alpha1 (PRKAA1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEXNKI-GST-3C-LIC

   Plasmid

   #108710

   Purpose

   Expression of a GST-tagged target protein with 3C protease cleavage site.

   Depositor

   Titia Sixma

   Article

   Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

   Type

   Empty backbone

   Use

   Tags

   GST-3C protease cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pYPQ292 (AaCas12b)-D570A-TV-MS2-TV

   Plasmid

   #136381

   Purpose

   CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system, followed by T2A and MCP-TV fusion protein

   Depositor

   Yiping Qi

   Article

   Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

   Insert

   AaCas12b (D570A)-TV-T2A-MCP-TV
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D570A

   Promoter

   Available since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMS6: pHelper(AvCAST)_entry_ΔTnsD

   Plasmid

   #168139

   Purpose

   Inducible expression of AvCAST proteins (except TnsD). Two BsaI sites for spacer cloning.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   AvCAST minimal CRISPR array
   AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
   AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDIRECT_23D

   Plasmid

   #91141

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:bar

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A, H840A

   Promoter

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Bxb1_jRCaMP1b

    Plasmid

    #183758

    Purpose

    Bxb1-specific donor plasmid for intergrating a transcription unit for expression of jRCaMP1b calcium reporter

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    jRCaMP1b
    

    Use

    Tags

    NES

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. DCT.VV.Orf196

    Plasmid

    #12884

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jon Yewdell, Jack Bennink

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf196
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pETNKI-strepII-3C-LIC-kan

    Plasmid

    #108706

    Purpose

    Expression of a StrepII-tagged target protein with 3C protease cleavage site.

    Depositor

    Titia Sixma

    Article

    Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

    Type

    Empty backbone

    Use

    Tags

    StrepII-3C protease cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SpyCas9 PE2-3HA

    Plasmid

    #169853

    Purpose

    Mammalian Expression, SpyCas9 prime editor with 3HA-tag

    Depositor

    Scot Wolfe

    Article

    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:

    Insert

    SpCas9-H840A-M-MLV-3HA
    

    Use

    Tags

    3XHA tag and bpSV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. BRD4 CRISPRi plasmid

    Plasmid

    #154890

    Purpose

    Human BRD4 CRISPRi gRNA

    Depositor

    Joseph Dougherty, Rob Mitra

    Article

    Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

    Insert

    BRD4-targeting gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. E1010m (RFP)_CD

    Plasmid

    #66033

    Purpose

    MoClo Basic Part: CDS - Fluorescent protein. Red. Modified from Bba_E1010 to fix illegal sites. [C:E1010m:D]

    Depositor

    Douglas Densmore

    Article

    Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

    Insert

    Fluorescent reporter - RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLL7.0-iRFP670-micro

    Plasmid

    #135954

    Purpose

    Expresses an iLID micro (SspB R73Q)-iRFP670 fusion in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    iLID micro (SspB R73Q)
    

    Use

    Lentiviral

    Tags

    iRFP670

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-sCAG-GluClβ.CreON

    Plasmid

    #196996

    Purpose

    Cre recombinase dependent expression of GluClv2.0 beta subunit. GluClβ contains a YFP tag. When co-expressed with GluClv2.0 alpha subunit, agonist (Ivermectin) induces neuronal silencing.

    Depositor

    David Bennett

    Article

    Middleton et al Pain. 2023 Jun 27. doi: 10.1097/j.pain.0

    Insert

    GluClβ -YFP
    

    Use

    AAV and Cre/Lox

    Tags

    YFP

    Expression

    Mutation

    Promoter

    short CAG

    Available since

    June 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. MAC_N_CDK8

    Plasmid

    #111685

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    CDK8 (CDK8 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKLV2-U6gRNA5(Nelfcd-g1)-PGKpuroBFP-W

    Plasmid

    #105025

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nelfcd , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nelfcd (Th1l Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pKLV2-U6gRNA5(Nelfcd-g2)-PGKpuroBFP-W

    Plasmid

    #105026

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nelfcd , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nelfcd (Th1l Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pDIRECT_25I

    Plasmid

    #91146

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9_dead + PvUbi1:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A, H840A

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MAC_N_GSK3B

    Plasmid

    #111687

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    GSK3B (GSK3B Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pVRa12_807

    Plasmid

    #49663

    Depositor

    Christopher Voigt

    Article

    Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

    Insert

    ECF12_807
    

    Use

    Synthetic Biology

    Tags

    His6-PreScission

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli

    Promoter

    pT7-lacO

    Available since

    Jan. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMOD_B2121

    Plasmid

    #91065

    Purpose

    Module B, Promoter: GmUbi, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    GmUbi

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pEY61

    Plasmid

    #191058

    Purpose

    acr-2(3.7k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    acr-2(3.7k) (acr-2 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSicoR-PTPN11i1

    Plasmid

    #59612

    Purpose

    Expression of shRNA against human PTPN11

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    PTPN11 (PTPN11 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

27. pcDNA3-HA-RIPK3 2KR(55,363)

    Plasmid

    #78811

    Purpose

    To overexpress RIPK3 2KR(55,363) in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K55R, K363R

    Promoter

    CMV

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDsRed2-C1-Ahi1

    Plasmid

    #30495

    Depositor

    Joseph Gleeson

    Article

    Lancaster et al Nat Med. 2009 Sep . 15(9):1046-54.

    Insert

    Ahi1 (AHI1 Human)
    

    Use

    Tags

    DsRed2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

29. pMOD_C2516

    Plasmid

    #91084

    Purpose

    Module C, Promoter: At7SL, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    At7SL

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. DCT.VV.Orf207

    Plasmid

    #12895

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jon Yewdell, Jack Bennink

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf207
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    *

    Promoter

    Available since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

31. R4pGWB601_UBQ10p-BirA(R118G)-YFP-NLS

    Plasmid

    #127365

    Purpose

    Binary vector for expressing nuclear BirA* (R118G)-YFP under the UBQ10 promoter in plants

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    BirA* (R118G mutant)
    

    Use

    Tags

    GS linker, NLS, V5, and YFP

    Expression

    Plant

    Mutation

    R118G

    Promoter

    Arabidopsis UBQ10 promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pJMP1341

    Plasmid

    #119272

    Purpose

    control sgRNA in cells lacking rfp

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Promoter

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pcDNA3-FLAG-RIPK1 K163R

    Plasmid

    #78844

    Purpose

    To overexpress RIPK1 K163R in Mammalian Cells

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK1 (RIPK1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    K163R

    Promoter

    CMV

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pSicoR-PTPN11i2

    Plasmid

    #59613

    Purpose

    Expression of shRNA against human PTPN11

    Depositor

    Miguel Ramalho-Santos

    Article

    Qin et al Cell. 2014 Jul 17;158(2):449-61. doi: 10

    Insert

    PTPN11 (PTPN11 Human)
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

35. pDIRECT_22D

    Plasmid

    #91136

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:npt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A, H840A

    Promoter

    Available since

    July 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAAV-FDIO-SYP-HRP

    Plasmid

    #117187

    Purpose

    Plasmid name in publication: pAAV-FDIO-SV-HRP. Peroxidase reporter for multiplexed electron microscopy labeling

    Depositor

    David Ginty

    Article

    Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

    Insert

    SYP-HRP
    

    Use

    AAV; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pOPINK-OTUD6A (OTU, aa 128-288)

    Plasmid

    #61417

    Purpose

    Expresses human OTUD6A (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD6A (OTUD6A Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-127.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pPTG-GG-sg2+sg3

    Plasmid

    #127199

    Purpose

    Plasmid expressing sgRNA2 (TACCACATTTGTAGAGGTT) & sgRNA3 (CAATGTATCTTATCATGTC) as polycistronic tRNA-gRNA from single U6 promoter

    Depositor

    Jordan Green

    Article

    Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

    Insert

    tRNA-gRNA
    

    Use

    Empty sgrna plasmid

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pK-SAN1-Flag

    Plasmid

    #117163

    Purpose

    Expresses Flag-tagged SAN1 nuclease in mammalian cells.

    Depositor

    Ian Macara

    Article

    Andrews et al Nat Commun. 2018 Jul 3;9(1):2592. doi: 1

    Insert

    SAN1 (FAM120B Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. DCT.VV.Orf166

    Plasmid

    #12854

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jon Yewdell, Jack Bennink

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf166
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

41. pAAV-IgK sp-HA-CD4-10aa linker-CIBN-NNES-NanoLuc

    Plasmid

    #125229

    Purpose

    Expresses transmembrane intracellular NanoLuc in mammalian cells

    Depositor

    Alice Ting

    Article

    Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

    Insert

    IgK sp-HA-CD4-10aa linker-CIBN-NNES-NanoLuc
    

    Use

    AAV

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. DCT.VV.Orf212

    Plasmid

    #12900

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jon Yewdell, Jack Bennink

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf212
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 29, 2006

    Availability

    Academic Institutions and Nonprofits only

43. pDIRECT_21D

    Plasmid

    #91131

    Purpose

    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Engineering Reagent: 35S:Csy4-P2A-AtCas9_dead + CmYLCV:gRNAs with Csy4 spacers
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A, H840A

    Promoter

    Available since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pOPINK-OTUD6B (OTU, aa 133-293)

    Plasmid

    #61419

    Purpose

    Expresses human OTUD6B (OTU domain) in E. coli.

    Depositor

    David Komander

    Article

    Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.

    Insert

    OTUD6B (OTUD6B Human)
    

    Use

    Tags

    His6-GST-3C

    Expression

    Bacterial

    Mutation

    Deleted aa 1-132.

    Promoter

    T7

    Available since

    March 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pMOD_B2519

    Plasmid

    #91076

    Purpose

    Module B, Promoter: OsU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    OsU3

    Available since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. MAC_N_BET1

    Plasmid

    #111683

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    BET1 (BET1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. Delta DED FLIP-L D/A

    Plasmid

    #13791

    Depositor

    Guy Salvesen

    Article

    Boatright et al Biochem J. 2004 Sep 1. 382(Pt 2):651-7.

    Insert

    DELTA DED FLIP LONG (CFLAR Human)
    

    Use

    Tags

    8 x His tag

    Expression

    Bacterial

    Mutation

    Delta DED removes first 186 residues Asp to Ala m…

    Promoter

    Available since

    Jan. 25, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. MAC_N_FBRL

    Plasmid

    #111686

    Purpose

    MAC-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    FBRL (FBL Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. DCT.VV.Orf176

    Plasmid

    #12864

    Purpose

    This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

    Depositor

    Jon Yewdell, Jack Bennink

    Article

    Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

    Insert

    VV.Orf176
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

50. pGEX-4T-1-RIPK3 4KR

    Plasmid

    #78828

    Purpose

    To purify GST-tagged recombinant RIPK3 4KR protein

    Depositor

    Jaewhan Song

    Article

    Seo et al Nat Cell Biol. 2016 Mar;18(3):291-302. d

    Insert

    RIPK3 (RIPK3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    K55R, K89R, K363R, K501R

    Promoter

    tac

    Available since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge