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pET-sCS4T+N

Plasmid

#28255

Depositor

Yawen Bai

Article

Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

Insert

sCS4T+N

Tags

His

Expression

Bacterial

Mutation

single chain CSE4-SCM3-H4 with Thrombin site, SCM…

Available Since

July 12, 2011

Availability

Academic Institutions and Nonprofits only
pET-sC4T

Plasmid

#28251

Depositor

Yawen Bai

Article

Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.

Insert

single chain Cse4-H4 with Thrombin site

Tags

His

Expression

Bacterial

Mutation

A thrombin cutting site between Cse4 and H4

Available Since

July 11, 2011

Availability

Academic Institutions and Nonprofits only
piHGpd/T22I

Plasmid

#1110

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Expression

Yeast

Mutation

HSP82 with threonine 22 changed to isoleucine; re…

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
piHGpd/G81S

Plasmid

#1111

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Expression

Yeast

Mutation

HSP82 with glycine 81 changed to serine; regulate…

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only

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piHGpd/G313S

Plasmid

#1114

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Expression

Yeast

Mutation

HSP82 with glycine 313 changed to serine; regulat…

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
piHGpd/E381K

Plasmid

#1115

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Expression

Yeast

Mutation

HSP82 with glutamic acid 381 changed to lysine; r…

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
piHGpd/A587T

Plasmid

#1116

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Expression

Yeast

Mutation

HSP82 with alanine 587 changed to threonine; regu…

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
piHGpd/A41V

Plasmid

#1117

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1999 Feb 16;96(

Insert

HSP82 (HSP82 Budding Yeast)

Expression

Yeast

Mutation

HSP82 with alanine 41 changed to valine; regulate…

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pILGFP4G

Plasmid

#104502

Purpose

Used to evaluate the expression output of Saccharomyces cerevisiae PGM2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

ScPGM2 promoter-yEGFP

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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pILGFP4K

Plasmid

#104506

Purpose

Used to evaluate the expression output of Saccharomyces paradoxus GAL1 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SpGAL1 promoter-yEGFP

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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pILGFP4O

Plasmid

#104510

Purpose

Used to evaluate the expression output of Saccharomyces paradoxus GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SpdGAL2 promoter-yEGFP

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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pILGFP4P

Plasmid

#104511

Purpose

Used to evaluate the expression output of Saccharomyces pastorianus GAL2 promoter with yEGFP used as the reporter gene

Depositor

Claudia Vickers

Article

Peng et al ACS Synth Biol. 2018 Feb 16;7(2):748-751

Insert

SptGAL2 promoter-yEGFP

Expression

Yeast

Availability

Academic Institutions and Nonprofits only

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NaVSp1 FL DeltaCTD pIRES

Plasmid

#128077

Purpose

Mammalian cell expression vector for NaVSp1 bacterial sodium channel. Residues 1-224.

Depositor

Dan Minor

Article

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Insert

NaVSp1

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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NaVAe1 FL DeltaCTD pIRES

Plasmid

#128078

Purpose

Mammalian cell expression vector for NaVAe1 bacterial sodium channel. Residues 1-241.

Depositor

Dan Minor

Article

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Insert

NaVAe1

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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NaVAe1 DeltaCC pIRES

Plasmid

#128086

Purpose

Mammalian cell expression vector for NaVAe1 1-264 chimera bacterial sodium channel.

Depositor

Dan Minor

Article

Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

Insert

NaVAe1

Expression

Mammalian

Availability

Academic Institutions and Nonprofits only

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pUAP1

Plasmid

#63674

Purpose

Accepts DNA sequences via BpiI cloning sites, resulting in Level 0 standard parts for Golden Gate cloning. Parts can be released with a user-defined 4-base-pair, 5 prime overhangs using BsaI.

Depositor

Nicola Patron

Article

Patron et al New Phytol. 2015 Jul 14. doi: 10.1111/np

Insert

RFP cloning selection cassette

Use

Synthetic Biology

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV_BiPVe3_ChR2_mCherry

Plasmid

#213941

Purpose

AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by PV+ basket cell-targeting enhancer. Alias: AAV_WDP0003_ChR2_mCherry

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has Service

AAV PHP.eB and AAV1

Insert

Channelrhodopsin 2-mCherry

Use

AAV

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_BiVIPe4_ChR2_mCherry

Plasmid

#213856

Purpose

AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by VIP interneuron-targeting enhancer. Alias: pAAV_YWE4.2_ChR2_mCherry

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has Service

AAV PHP.eB and AAV1

Insert

Channelrhodopsin 2-mCherry

Use

AAV

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_BiSSTe4_ChR2_mCherry

Plasmid

#213945

Purpose

AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by SST interneuron-targeting enhancer. Alias: pAAV_WDS0004_ChR2_mCherry

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has Service

AAV PHP.eB and AAV1

Insert

Channelrhodopsin 2-mCherry

Use

AAV

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_BiLAMP5e3_ChR2_mCherry

Plasmid

#213915

Purpose

AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by Lamp5 interneuron-targeting enhancer. Alias: pAAV_WDL0003_ChR2_mCherry

Depositor

Gordon Fishell

Article

Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

Has Service

AAV PHP.eB and AAV1

Insert

Channelrhodopsin 2-mCherry

Use

AAV

Available Since

July 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge