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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,350 results for: 11

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Showing: 3141 - 3160 of 8350 results

  3. pCRI008-PgpdA-Cas12aScaffold-BsmbI-Cas12aScaffold-TrcpT

    Plasmid
    #140201
    Purpose
    Fungal vector for one-step cloning of LbCas12a crRNA arrays and expression. Pgpda and pyroA are BsmbI domesticated.
    Depositor
    Yit Heng Chooi
    Publication
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology; Cloning of crrna an…
    Tags
    dLbCas12a crRNA scaffold
    Promoter
    PgpdA
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140201-plasmid-map-sequence-id-310178

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  5. pT2Apuro-TRE-synPCB2.0

    Plasmid
    #139481
    Purpose
    Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.
    Depositor
    Kazuhiro Aoki
    Publication
    Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7
    Use
    Tol2 transposon donor vector
    Expression
    Mammalian
    Promoter
    Tet responsive element
    Available since
    March 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    139481-plasmid-map-sequence-id-276016

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  6. sfGFP-Cx43K258stop

    Plasmid
    #69025
    Purpose
    Expresses rat Cx43 (Gja1 CDS) truncated at amino acid 258 and tagged with sfGFP (V206-version) on the N-terminus. CMV promoter.
    Depositor
    David Spray
    Publication
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Insert
    Cx43 (Gja1 Rat)
    Tags
    super folder GFP
    Expression
    Mammalian
    Mutation
    non-monomerized sfGFP fused in frame to N-terminu…
    Promoter
    CMV
    Available since
    Oct. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    69025-plasmid-map-sequence-id-127523

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  10. pcDNA-NLS-ZapCmR1.1

    Plasmid
    #59011
    Purpose
    Nuclear zinc-binding FRET biosensor
    Depositor
    Amy Palmer
    Publication
    Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NLS-ZapCmR1.1 genetically encoded Zn(II) sensor
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    See comment
    Promoter
    CMV
    Available since
    Aug. 21, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. pCR4-Dact1(1250-1601)

    Plasmid
    #52046
    Purpose
    To generate mRNA in situ probe against mouse Dact1 (Probe C in PMID 17013874). For antisense cut with Spe1, transcribe with T7. For sense cut with Not1, treat with T4 polymerase, transcribe with T3.
    Depositor
    Ben Cheyette
    Publication
    Suriben et al Dev Dyn. 2006 Nov;235(11):3177-83.
    Insert
    Dact1(1250-1601) (Dact1 Mouse)
    Expression
    Bacterial
    Available since
    April 18, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. pAAV-Ef1a-fDIO EYFP (AAV5)

    Viral prep
    #55641-AAV5
    Purpose
    Ready-to-use AAV5 particles produced from pAAV-Ef1a-fDIO EYFP (#55641). In addition to the viral particles, you will also receive purified pAAV-Ef1a-fDIO EYFP plasmid DNA. EF1a-driven, Flp-dependent expression of EYFP. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Publication
    Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:
    Promoter
    EF1a
    Tags
    EYFP (Flp-dependent)
    Available since
    Oct. 21, 2024
    Availability
    Academic Institutions and Nonprofits only

  19. Sc GST-P-Pol32-pLANT(2b)/RIL

    Plasmid
    #257903
    Purpose
    Over-express Sc GST/P-Pol32 in E. coli. To be used in conjunction with Sc Pol31-pET(16b) (no tag) (Addgene #257902) to over-express Sc [Pol31 + GST/P-Pol32] complex in E. coli.
    Depositor
    Michael O'Donnell
    Insert
    Sc Pol32
    Tags
    GST tag followed by PreScission Protease site
    Expression
    Bacterial
    Available since
    July 28, 2026
    Availability
    Academic Institutions and Nonprofits only
    257903-plasmid-map-sequence-id-516870

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  20. Sc Pol31-pET(16b) (no tag)

    Plasmid
    #257902
    Purpose
    Over-express Sc Pol31 in E. coli. To be used in conjunction with Sc GST-P-Pol32-pLANT(2b)/RIL (Addgene #257903) to over-express Sc [Pol31 + GST/P-Pol32] complex in E. coli.
    Depositor
    Michael O'Donnell
    Insert
    Sc Pol31
    Expression
    Bacterial
    Available since
    June 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    257902-plasmid-map-sequence-id-513600

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Showing: 3141 - 3160 of 8350 results