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pACYCDuet1-ammA(∆50-56)

Plasmid

#134684

Purpose

Expresses His6-AmmA(∆50-56) in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

ammA(del50-56)

Tags

His6

Expression

Bacterial

Mutation

deletion of amino acids 50-56

Promoter

T7

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pACYCDuet1-ammA

Plasmid

#134682

Purpose

Expresses His6-AmmA in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

ammA

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET28b-tglF

Plasmid

#134681

Purpose

Expresses His6-TglF in E. Coli

Depositor

Wilfred van der Donk

Article

Ting et al Science. 2019 Jul 19;365(6450):280-284.

Insert

TglF

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

July 14, 2020

Availability

Academic Institutions and Nonprofits only

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pmKaxxte

Plasmid

#113630

Purpose

to test Cas9 gRNA efficiency or HR-capacity

Depositor

Ken Mills

Article

Liberante et al Sci Rep. 2019 Feb 25;9(1):2678. doi: 10.

Insert

mKaxxte2.5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pOZ-FH-C Bach1 K141A/K142A

Plasmid

#73646

Purpose

Retroviral expression of Bach1 K141A/K142A mutant (mlh1 binding mutant)

Depositor

Sharon Cantor

Insert

Bach1 (BACH1 Human)

Use

Retroviral

Tags

FLAG and His

Expression

Mammalian

Mutation

K141A/K142A ( mlh1 binding mutant)

Promoter

unknown

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only
pCfB4779

Plasmid

#106145

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_2, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB4784

Plasmid

#106148

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntF_2, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB4785

Plasmid

#106149

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntF_3, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB4783

Plasmid

#106146

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntE_3, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB4781

Plasmid

#106140

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntC_1, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pCfB4788

Plasmid

#106139

Purpose

EasyCloneYALI system-based yeast integrative vector carrying loxP-flanked nourseothricin-resistance marker, integration into Yarrowia lipolytica chromosomal location IntA_1, USER site for cloning, amp resistance

Depositor

Irina Borodina

Article

Holkenbrink et al Biotechnol J. 2018 Jan 29. doi: 10.1002/

Type

Empty backbone

Expression

Yeast

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pME-Fv2E-flt4-ns

Plasmid

#67695

Purpose

middle entry plasmid with dimerizable extracellular domain fused to Danio Flt4 cytoplasmic domain

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

Fv2E-flt4

Mutation

no stop codon; D1302E (see depositor's comme…

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only
MS4A1 RFP (805)

Plasmid

#62009

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Nucleus. 2012 Nov-Dec;3(6):552-64. doi:

Insert

MS4A1

Tags

mRFP

Expression

Mammalian

Promoter

pgk

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pHL1633

Plasmid

#62830

Purpose

Lim Lab plasmid: AmpR + PLlacO-1::T710 gfp::Asp terminator + ColE1

Depositor

Han Lim

Article

Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

Insert

gfp

Use

Synthetic Biology

Tags

T710

Expression

Bacterial

Promoter

pLlacO-1

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
pAN-PBAD-sgRNA-VR

Plasmid

#62286

Purpose

expression of VR sgRNA from the arabinose-inducible promoter

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

VR sgRNA

Use

CRISPR

Expression

Bacterial

Promoter

pBAD

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pAN-PA5-RFP

Plasmid

#62251

Purpose

reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

mRFP

Expression

Bacterial

Promoter

pA5

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pHL1801

Plasmid

#53039

Purpose

PLlacO-1:RBS(st7):gfp

Depositor

Han Lim

Article

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Insert

GFP

Use

Synthetic Biology

Tags

RBS(st7)

Expression

Bacterial

Promoter

pLlac0-1

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pHL 342

Plasmid

#53012

Purpose

PLtetO-1:mCherry, PLlacO-1:araC, Plar:ompC::gfp

Depositor

Han Lim

Article

Hussein et al Nucleic Acids Res. 2012 May 22.

Inserts

mCherry
araC
ompC

Use

Synthetic Biology

Tags

gfp

Expression

Bacterial

Mutation

contains -81 to +36 relative to start codon

Promoter

Plar, pLlacO-1, and pLtetO-1

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
pHL1506

Plasmid

#53026

Purpose

PLlacO-1:RBS(st7):csrA

Depositor

Han Lim

Article

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Insert

csrA

Use

Synthetic Biology

Tags

RBS(st7)

Expression

Bacterial

Promoter

pLlac0-1

Available Since

May 13, 2014

Availability

Academic Institutions and Nonprofits only
pHAGE-mt-mKeima

Plasmid

#131626

Purpose

lentiviral expression of mitochondrial mKeima for generation of stable cell lines

Depositor

Richard Youle

Article

Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

Insert

mt-mKeima

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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