We narrowed to 59,421 results for: cloning

1. pCIG3 (pCMV-IRES-GFP version 3)

   Plasmid

   #78264

   Purpose

   Lentiviral empty cloning vector containing IRES and GFP

   Depositor

   Felicia Goodrum

   Article

   Caviness et al J Virol. 2014 Dec;88(24):14412-25. doi:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Promoter

   CMV, lentiviral LTR

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMOD_B2520

   Plasmid

   #91077

   Purpose

   Module B, Promoter: OsU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Esp3I ccdb cassette for single gRNA spacer cloning
   

   Use

   CRISPR

   Promoter

   OsU6

   Available Since

   July 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pX330-BsaIx2-DD-Cas9

   Plasmid

   #75380

   Purpose

   Expresses DD-SpCas9 in mammalian cells and has a cloning site for an sgRNA. The FKBP12 L106P destabilization domain allows for inducible stabilization of Cas9 through the addition of Shield-1

   Depositor

   Michele Calos

   Article

   Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

   Insert

   DD-SpCas9
   

   Use

   CRISPR

   Tags

   FKBP12 L106P DD

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pENTR221-NR1H2

   Plasmid

   #79513

   Purpose

   MultiSite Gateway entry clones, second fragment (attL5, attL2) Human NR1H2 CDS

   Depositor

   Peter Jon Nelson

   Article

   Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

   Insert

   LXR BETA (NR1H2 Human)
   

   Use

   Multisite gateway entry clone

   Promoter

   no Promoter

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. STAGR_gRNAScaffold_mU6

   Plasmid

   #102844

   Purpose

   Can be used as PCR template for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Insert

   STAgR Insert gRNAScaffold_mU6
   

   Use

   Pcr template for stagr inserts

   Promoter

   mU6

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. STAGR_gRNAScaffold_hH1

   Plasmid

   #102841

   Purpose

   Can be used as PCR template for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Insert

   STAgR Insert gRNAScaffold_hH1
   

   Use

   Pcr template for stagr inserts

   Promoter

   hH1

   Available Since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDONR221_HUWE1_isoform1

   Plasmid

   #190464

   Purpose

   Entry clone

   Depositor

   Eric Fischer

   Article

   Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

   Insert

   HUWE1 (HUWE1 Human)
   

   Use

   Entry clone

   Available Since

   Sept. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. JC201: pMVP/Ad/5-35-DEST

   Plasmid

   #121847

   Purpose

   pMVP destination vector, empty 5/35 fiber-modified adenovirus vector backbone

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Adenoviral; Pmvp gateway destination vector

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSG4K5

   Plasmid

   #74492

   Purpose

   For cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.

   Depositor

   Xiao Wang

   Article

   Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2

   Insert

   sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p3E-2xFYVE_hrs

    Plasmid

    #67676

    Purpose

    Multisite gateway entry clone for adding C-terminal fusions of 2xFYVE domain from hrs

    Depositor

    Rob Parton

    Article

    Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

    Insert

    2xFYVEhrs
    

    Use

    Gateway multisite 3' entry clone

    Available Since

    Dec. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. LC001: pMVP/PB/Hygro-DEST

    Plasmid

    #121876

    Purpose

    pMVP destination vector, empty PiggyBac transposon vector backbone w/ Hygro selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Piggybac transposon, pmvp gateway destination vec…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. LB601: pMVP/PB/eGFP-DEST

    Plasmid

    #121870

    Purpose

    pMVP destination vector, empty PiggyBac transposon vector backbone w/ eGFP selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Piggybac transposon, pmvp gateway destination vec…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MSGV Mouse Acceptor PGK NGFR

    Plasmid

    #128482

    Purpose

    A TCR Variable Region cloning expression vector using Mouse Constant Regions to reduce mis-pairing with endogenous TCR expressing NGFR ref Biotechniques. 2015 Mar 1;58(3):135-9

    Depositor

    David Ott

    Article

    Coren et al Biotechniques. 2015 Mar 1;58(3):135-9. d

    Type

    Empty backbone

    Use

    Retroviral

    Promoter

    MSCV

    Available Since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. II701: pMVP (L1-L4) cTnT promoter

    Plasmid

    #121690

    Purpose

    pMVP L1-L4 entry plasmid, contains cTnT promoter for 3-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    cTnT promoter (Cardiomyocyte specific)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. LC201: pMVP/PB/Puro-DEST

    Plasmid

    #121874

    Purpose

    pMVP destination vector, empty PiggyBac transposon vector backbone w/ Puro selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Piggybac transposon, pmvp gateway destination vec…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pPM1003

    Plasmid

    #167550

    Purpose

    Encodes genes that confer expression of Vibrio cholerae O antigens of the Ogawa serotype in E. coli K-12

    Depositor

    Renato Morona

    Article

    Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

    Insert

    Vibrio cholerae O antigens genes of the Ogawa serotype
    

    Use

    Cosmid

    Available Since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSG058

    Plasmid

    #214261

    Purpose

    PaFtsH1-6xHis cloned between XbaI/NheI in pET-28a(+)

    Depositor

    Ute Romling

    Article

    Mawla et al J Biol Chem. 2024 Feb;300(2):105622. doi

    Insert

    Core genome PaFtsH1 proteinase from Pseudomonas aeruginosa SG17M
    

    Tags

    6xHis-tag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMOD_B2515

    Plasmid

    #91072

    Purpose

    Module B, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, Terminator: Pol III

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Esp3I ccdb cassette for single gRNA spacer cloning
    

    Use

    CRISPR

    Promoter

    AtU6

    Available Since

    July 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDONR223 LC3B WT

    Plasmid

    #123072

    Purpose

    Gateway entry clone encoding human MAP1LC3B wild-type

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Use

    Gateway entry vector / entry clone

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pENTR-D-TOPO-PAmCherry1-MCS

    Plasmid

    #60608

    Purpose

    Cloning PAmCherry1

    Depositor

    Xiaolin Nan, Steven Chu

    Article

    Nan et al Proc Natl Acad Sci U S A. 2013 Nov 12;11

    Insert

    PAmCherry1
    

    Use

    Cloning

    Available Since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge