We narrowed to 12,734 results for: icos

1. pcDNA3.1-nAChr-Alpha6-mCherry

   Plasmid

   #50480

   Purpose

   this report is the first to directly measure nAChr subunit stoichiometry using FRET and plasma membrane localization of Alpha6 and Beta3 containing receptors using TIRF

   Depositor

   Henry Lester

   Article

   Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

   Insert

   nAChr-alpha6 (Chrna6 Mouse)
   

   Use

   Tags

   mCherry fusion in M3-M4 loop (after residue A405)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pRRL_SRSF2_P95H_mCherry

   Plasmid

   #84023

   Purpose

   expression of SRSF2 mutant in mammalian cells

   Depositor

   Robert Bradley

   Article

   Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

   Insert

   SRSF2 (SRSF2 Human)
   

   Use

   Lentiviral

   Tags

   2TA-mCherry and Flag

   Expression

   Mammalian

   Mutation

   P95H

   Promoter

   PGK

   Available since

   Oct. 17, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-GRAB_CRF1.0

   Plasmid

   #208658

   Purpose

   Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in neurons

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. iYFP-Parkin Plasmid

   Plasmid

   #197107

   Purpose

   Expresses YFP-tagged Parkin when induced by Doxycycline in mammalian cells.

   Depositor

   Dori Woods

   Article

   Capelluto et al Adv Biol (Weinh). 2023 Jan 18:e2200246.

   Insert

   parkinson protein 2, E3 ubiquitin protein ligase (PRKN Human)
   

   Use

   Retroviral

   Tags

   YFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC119-gRNA

   Plasmid

   #52255

   Purpose

   Can use a PCR template to assemble new desired guide RNA. Contains an Arabidopsis U6 promoter to drive guide RNA (targeting AtPDS3 gene target site 1) expression with a TTTTTT as terminator.

   Depositor

   Jen Sheen

   Article

   Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d

   Insert

   guide RNA targeting AtPDS3 (PDS3 Mustard Weed)
   

   Use

   CRISPR; Plant expression

   Tags

   Expression

   Mutation

   Promoter

   Arabidopsis U6 polymerase III promoter

   Available since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMCB306-GFP-mito

   Plasmid

   #229232

   Purpose

   Expresses GFP localized to mitochondria

   Depositor

   Suzanne Pfeffer

   Article

   Bagnoli et al Proc Natl Acad Sci U S A. 2025 Feb 4;122

   Insert

   EGFP-MAOA (MAOA Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pYLCRISPR/Cas9Pubi-B

   Plasmid

   #66188

   Purpose

   expression of Cas9 in plants, maize ubiquitin promoter (Pubi), basta selection

   Depositor

   Yao-Guang Liu

   Article

   Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

   Insert

   Cas9
   

   Use

   Tags

   Expression

   Plant

   Mutation

   plant-codon optimized with higher GC contents (62…

   Promoter

   maize ubiquitin promoter (Pubi)

   Available since

   Sept. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAS_4xMmaPylT_EF1_CRFR1-A95TAG-HA

   Plasmid

   #174900

   Purpose

   amber suppression mediated CRFR1 A95TAG expression in mammalian cells; transient or piggy bac mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Front Chem. 2021 Nov 11;9:768535. doi: 1

   Insert

   CRFR1 (Crhr1 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   A95TAG

   Promoter

   EF1

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. ACTB-(A)4x

   Plasmid

   #112058

   Purpose

   ACTB mRNA tagged with 4 copies of Riboglow (variant A)

   Depositor

   Amy Palmer

   Article

   Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

   Insert

   ACTB (ACTB Human)
   

   Use

   Tags

   Riboglow RNA tag

   Expression

   Mammalian

   Mutation

   4 copies of Riboglow tag after stop codon

   Promoter

   CMV

   Available since

   July 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. hum alpha ENAC promoter a25-a23

    Plasmid

    #83427

    Purpose

    promoter reporter construct to test 5' flanking sequences

    Depositor

    Christie Thomas

    Article

    Sayegh et al J Biol Chem. 1999 Apr 30;274(18):12431-7

    Insert

    human aENaC gene 5' flanking seq + 55 nt of the 5' UTR for exon 1A (SCNN1A Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mutation

    Promoter

    alpha ENAC

    Available since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAB(EXPR-PYL-HDAC3-NEO)

    Plasmid

    #114394

    Purpose

    For PYL-HDAC3 expression

    Depositor

    Vincent Dion

    Article

    Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

    Insert

    PYL-HDAC3 (HDAC3 Human)
    

    Use

    Tags

    3xFlag-NLS (internal)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. sgTAZ-R-3

    Plasmid

    #229450

    Purpose

    knockout of WWTR1 (TAZ)

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    sgTAZ-R-3 (WWTR1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6;bU6;EFS

    Available since

    Dec. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYLCRISPR/Cas9pUbi-N

    Plasmid

    #106331

    Purpose

    Expression of Cas9 in plants, ZmUbi promoter, Kanamycin selection

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    plant-codon optimized with higher GC contents (62…

    Promoter

    ZmUbi

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1+FLAG-KrasnatG12D

    Plasmid

    #206842

    Purpose

    To express mouse Kras encoded by native mouse codons and with a G12D mutation with an N-terminal FLAG tag.

    Depositor

    Christopher Counter

    Article

    Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

    Insert

    Kras (Kras Mouse)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Glycine 12 to Aspartic Acid

    Promoter

    Available since

    Oct. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. FLAG-MARK3-CR

    Plasmid

    #229422

    Purpose

    Lentiviral or overpexression of cDNA

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    MARK3-CR (MARK3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    silent mutation CRISPR resistant

    Promoter

    EFS

    Available since

    Dec. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSyn-DIO-GRAB_CRF1.0

    Plasmid

    #208661

    Purpose

    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in a cre-dependent manner

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ACTB-(A)1x

    Plasmid

    #112055

    Purpose

    ACTB mRNA tagged with 1 copy of Riboglow (variant A)

    Depositor

    Amy Palmer

    Article

    Braselmann et al Nat Chem Biol. 2018 Jul 30. pii: 10.1038

    Insert

    ACTB (ACTB Human)
    

    Use

    Tags

    Riboglow RNA tag

    Expression

    Mammalian

    Mutation

    1 copy of Riboglow tag (variant A) after stop cod…

    Promoter

    CMV

    Available since

    June 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKrox24(MapERK)d1EGFP (KroxDs)

    Plasmid

    #214912

    Purpose

    for PiggyBac mediated integration and stable expression of destabilised EGFP as a reporter to MAPK/ERK pathway activation

    Depositor

    Pavel Krejčí

    Article

    Zlinska et al Mol Ther Nucleic Acids. 2024 Nov 28;36(1

    Insert

    d1EGFP under the MapERK promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    MapkERK promoter

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hSyn-GRAB_CRFmut

    Plasmid

    #208659

    Purpose

    Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) control sensor GRAB_CRFmut in neurons

    Depositor

    Yulong Li

    Article

    Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

    Insert

    GPCR activation based corticotropin-releasing factor (CRF) contorl sensor GRAB_CRFmut
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP

    Plasmid

    #171090

    Purpose

    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus thermophilus (CRISPR1 locus) in Toxoplasma gondii. Suitable for generating stable cell lines.

    Depositor

    Sebastian Lourido

    Article

    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1

    Inserts

    Sth1 dCas9
    Chloramphenicol acetyltransferase
    TagBFP
    

    Use

    CRISPR; Toxoplasma gondii expression

    Tags

    3xFLAG, P2A peptide, SV40 NLS, T2A peptide, and n…

    Expression

    Mutation

    D9A, H599A

    Promoter

    TUB1 (Toxoplasma gondii), transcriptionally linke…

    Available since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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