We narrowed to 12,213 results for: nsf

Showing: 3141 - 3160 of 12213 results

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pCSCG-Fc-pST3Gal1 WT

Plasmid

#228819

Purpose

Expresses Fc-fusion pST3Gal1 WT in mammalian cells

Depositor

Sriram Neelamegham

Article

Hombu et al Nat Commun. 2025 Jul 18;16(1):6637. doi:

Insert

Fc-pST3Gal1 WT

Use

Lentiviral

Tags

6His tag and human IgG1 Fc region

Expression

Mammalian

Mutation

amino acids 1-59 of pST3Gal1 were deleted

Promoter

CMV

Available Since

Aug. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pBN415

Plasmid

#217920

Purpose

express E. coli E69 (O9a:K30) Wzc with N711Y mutation

Depositor

James Naismith

Article

Yang et al Nat Commun. 2025 Apr 11;16(1):3437. doi:

Insert

E. coli E69 (O9a:K30) Wzc with N711Y mutation

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

N711Y

Available Since

July 10, 2025

Availability

Academic Institutions and Nonprofits only

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ADEPT-pCas9

Plasmid

#238032

Purpose

Encodes Cas9 (ssrA degradation-tagged) under a tetracycline promoter (pTet) on a low-copy replication origin (SC101) as part of the ADEPT system.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

spCas9

Use

Synthetic Biology

Tags

ssrA

Promoter

tet

Available Since

June 12, 2025

Availability

Academic Institutions and Nonprofits only

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ADEPT-pTarget-Msp4

Plasmid

#238037

Purpose

Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Promoter

lac

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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ADEPT-pTarget-Msp1

Plasmid

#238034

Purpose

Encodes sfGFPunder lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Promoter

lac

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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ADEPT-pTarget-Msp2

Plasmid

#238035

Purpose

Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Promoter

lac

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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ADEPT-pTarget-Msp3

Plasmid

#238036

Purpose

Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Promoter

lac

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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ADEPT-pTarget-Msp6

Plasmid

#238039

Purpose

Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Promoter

lac

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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ADEPT-pTarget-Msp5

Plasmid

#238038

Purpose

Encodes sfGFP under lac promoter. Expresses a self-targeting mismatched guide RNA as part of the ADEPT system. Carries oriT and kanamycin resistance.

Depositor

Lingchong You

Article

Son et al Nat Chem Biol. 2025 Jan 8. doi: 10.1038/

Insert

sfGFP

Use

Synthetic Biology

Promoter

lac

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ dgat1a-FLAG

Plasmid

#232143

Purpose

Expresses zebrafish dgat1a with a C-terminal FLAG tag under control of the CMV promoter

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

dgat1a (dgat1a Zebrafish)

Use

Zebrafish expression

Tags

FLAG

Promoter

CMV

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO-dgat1b in situ probe

Plasmid

#232138

Purpose

zebrafish dgat1b anti-sense in situ probe, linearize with NotI, SP6 polymerase

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

dgat1b

Use

In vitro transcription

Promoter

SP6/T7

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pCRII-TOPO-mogat2 in situ probe

Plasmid

#232139

Purpose

zebrafish mogat2 anti-sense in situ probe, linearize with NotI, SP6 polymerase

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

mogat2 (mogat2 Zebrafish)

Use

In vitro transcription

Promoter

SP6/T7

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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pAHW/Joseph2-Multitagged Plasmid

Plasmid

#226725

Purpose

Validates the expression and detection of epitope-tagged proteins of interest in Drosophila cells.

Depositor

Richard Cripps

Article

Robinson et al Biotechniques. 2024;76(7):299-309. doi:

Insert

pAHW/Joseph2

Use

Drosophila s2 cell expression

Tags

HA, Myc, GFP, Flag, V5, 6xHis

Expression

Insect

Promoter

Act5C

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pEE844

Plasmid

#231167

Purpose

T-DNA encoding TRV2 with PaqCI Recognition Sites and Ccdb

Depositor

Daniel Voytas

Article

Liu et al Proc Natl Acad Sci U S A. 2024 Sep 24;12

Type

Empty backbone

Use

Cloning vector

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pET28a-CsHPAD-AE

Plasmid

#226982

Purpose

Expressing Clostridium scatologenes hydroxyphenylacetate decarboxylase activating enzyme (HPAD-AE) in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

CsHPAD-AE

Tags

His6 (on backbone)

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD-AE

Plasmid

#226968

Purpose

Expressing Olsenella uli indoleacetate decarboxylase activating enzyme (IAD-AE) in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD-AE

Tags

His6 (on backbone)

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-OuIAD

Plasmid

#226967

Purpose

Expressing Olsenella uli indoleacetate decarboxylase (IAD) in E. coli

Depositor

Emily Balskus

Article

Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

Insert

OuIAD

Tags

His6 (on backbone)

Expression

Bacterial

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pUCmini-UGCG-EGFP

Plasmid

#225878

Purpose

Plasmid donor template for CRISPR/Cas9 mediated UGCG knockout by EGFP insertion

Depositor

Yohei Sato

Article

Sato et al Biochem Biophys Res Commun. 2024 Sep 18;

Insert

EGFP

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLVUT-spGFP

Plasmid

#225486

Purpose

Tet-on lentiviral vector for controlled expression of secretable and permeable EGFP

Depositor

Robert Gross

Article

Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d

Insert

Igκ leader and TAT peptide

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

Human ubiquitin C

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-nef-AO-66-71-TVA950

Plasmid

#217977

Purpose

AAV Transfer plasmid to express TVA950 in a Cre-dependent manner

Depositor

Edward Callaway

Article

Patino et al Elife. 2023 Dec 14;12:e89297. doi: 10.75

Insert

TVA950

Use

AAV

Promoter

nEF

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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