We narrowed to 16,799 results for: puromycin

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MSCV JMJD3

Plasmid

#21212

Depositor

Paul Khavari

Article

Sen et al Genes Dev. 2008 Jul 15. 22(14):1865-70.

Insert

JMJD3 (KDM6B Human)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Available Since

May 22, 2009

Availability

Academic Institutions and Nonprofits only

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AAVS1 EGFP-p62 HRD

Plasmid

#207550

Purpose

Homologous recombination donor to integrate and expression cassette for eGFP-p62 in the AAVS1 locus of human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

TET inducible EGFP-P62

Tags

EGFP

Expression

Mammalian

Promoter

Endogenous

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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AAVS1 EGFP-LC3B HRD

Plasmid

#207551

Purpose

Homologous recombination donor to integrate and expression cassette for eGFP-LC3B in the AAVS1 locus of human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

TET inducible EGFP-LC3B

Tags

EGFP

Expression

Mammalian

Promoter

Endogenous

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1 EGFP-GABARAPL1 HRD

Plasmid

#207552

Purpose

Homologous recombination donor to integrate and expression cassette for EGFP-GABRAPL1 in the AAVS1 locus of human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

TET inducible EGFP-GABARAPL1

Tags

EGFP

Expression

Mammalian

Promoter

Endogenous

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pEM705 Gsi (Cys)

Plasmid

#109373

Purpose

GsX chimera with Gαi C-terminus (Pertussis toxin sensitive)

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gsi chimera

Tags

None

Expression

Mammalian

Mutation

Cys at position 391 (Pertussis Toxin Sensitive)

Promoter

CAG

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO-PTEN-shRNA-3001

Plasmid

#25639

Depositor

Todd Waldman

Article

Kim et al Mol Cell Biol. 2007 Jan . 27(2):662-77.

Insert

PTEN-shRNA-3001 (PTEN Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pEM705 Gst (Cys)

Plasmid

#109376

Purpose

GsX chimera with Gαt C-terminus (Pertussis toxin sensitive)

Depositor

Robert Lucas

Article

Ballister et al BMC Biol. 2018 Jan 16;16(1):10. doi: 10.

Insert

Gst chimera

Tags

None

Expression

Mammalian

Mutation

Cys at position 391 (Pertussis Toxin Sensitive)

Promoter

CAG

Available Since

May 14, 2018

Availability

Academic Institutions and Nonprofits only

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FRT LAMP3 mCRISPRed_mTagBFP2 pcDNA5

Plasmid

#159455

Purpose

Encodes a lysosome tagret fusion protein of the pH resistant proteins mCRISPRed and mTagBFP2

Depositor

Oliver Griesbeck

Article

Erdogan et al Cell Chem Biol. 2020 May 21;27(5):610-62

Insert

mCRISPRed

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX-FHH-Empty Vector-IRES-Puro

Plasmid

#120568

Purpose

For cloning genes with an N-terminal TAP tag [Flag-HA-6xHIS]

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Type

Empty backbone

Use

Lentiviral

Tags

Flag-HA-6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Puro-3xHA

Plasmid

#207696

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-3xHA cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-Tight-Puro FLAG-SMAD4 TetON

Plasmid

#107129

Purpose

Inducible expression of Flag-tagged SMAD4 in mammalian cells

Depositor

Joan Massague

Article

David et al Cell. 2016 Feb 25;164(5):1015-30. doi: 1

Insert

SMAD4

Use

Lentiviral

Tags

FLAG

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pmRFP_a_tubulin_IRES_puro2b

Plasmid

#21041

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

alpha Tubulin (TUBA1B Human)

Tags

pmRFP

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2009

Availability

Academic Institutions and Nonprofits only

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Flag-HA-USP21

Plasmid

#22574

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP21 (USP21 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pMXs-IP GFP-WIPI-1

Plasmid

#38272

Depositor

Noboru Mizushima

Article

Itakura et al Autophagy. 2010 Aug;6(6):764-76.

Insert

WD repeat domain phosphoinositide-interacting protein 1 (WIPI1 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 16, 2012

Availability

Academic Institutions and Nonprofits only

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pME-puro-SNAP-FLAG-CD59

Plasmid

#50374

Purpose

Express SNAP and FLAG-tagged human CD59, a GPI-AP in mammmalian cells

Depositor

Reika Watanabe

Article

Castillon et al Mol Biol Cell. 2013 Jun;24(12):2021-33.

Insert

SNAP

Expression

Mammalian

Promoter

SR alpha

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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pJR152

Plasmid

#196280

Purpose

Cassette used in dual sgRNA library generation

Depositor

Rebecca Voorhees

Article

Guna et al BMC Genomics. 2023 Oct 30;24(1):651. doi

Insert

hU6-sgRNA-CR3

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV JMJD3 mutant

Plasmid

#21214

Depositor

Paul Khavari

Article

Sen et al Genes Dev. 2008 Jul 15. 22(14):1865-70.

Insert

JMJD3 (KDM6B Human)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Mutation

H1390A

Available Since

June 23, 2009

Availability

Academic Institutions and Nonprofits only

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AAVS1 EGFP-Sec61B

Plasmid

#207553

Purpose

Homologous recombination donor to integrate and expression cassette for EGFP-Sec61B in the AAVS1 locus of human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

TET inducible EGFP-Sec61B

Tags

EGFP

Expression

Mammalian

Promoter

Endogenous

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
PLKO.1-UPF1-CDS

Plasmid

#136037

Purpose

UPF1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (AAGACACCTATTACACGAAGG)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF1 (UPF1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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Flag pLJM1 RagB wt

Plasmid

#19313

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding B (RRAGB Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge