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act-AsCpf1

Plasmid

#73917

Purpose

expression plasmid of AsCpf1

Depositor

Simon Bullock

Article

Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

Insert

hAsCpf1

Tags

NLS-3xHA

Expression

Insect

Promoter

act5C

Available Since

March 8, 2016

Availability

Academic Institutions and Nonprofits only

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pBBRBB-Ppuf843-1200-PR

Plasmid

#50963

Purpose

Expresses proteorhodopsin using the Ppuf843-1200 promoter segment, for use in R. sphaeroides

Depositor

Claudia Schmidt-dannert

Article

Tikh et al Appl Microbiol Biotechnol. 2014 Feb 9.

Insert

Proteorhodopsin

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Promoter

Ppuf843-1200

Available Since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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GluA3 (px330:2-guideCas9)

Plasmid

#169424

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Expression

Mammalian

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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vgCam-pRSETb

Plasmid

#193362

Purpose

A FRET-based calcium probe using Sumire

Depositor

Takeharu Nagai

Article

Sugiura et al Commun Biol. 2022 Nov 3;5(1):1172. doi:

Insert

vgCam

Use

E.coli expression

Tags

His X6 tag and Strep tag

Promoter

T7

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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p426*-LbSgH

Plasmid

#136992

Purpose

Helper plasmid for LbCpf1 gRNA cloning and expression

Depositor

Huimin Zhao

Article

Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

Type

Empty backbone

Expression

Yeast

Promoter

SNR52

Available Since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pSUPER.PKCzeta.RNAi

Plasmid

#10803

Depositor

Alex Toker

Article

Storz et al Mol Cell Biol. 2004 Apr . 24(7):2614-26.

Insert

PKC zeta RNAi (PRKCZ Human)

Use

RNAi

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only
pSP64-Lifeact-GFP

Plasmid

#135601

Purpose

Expression of Lifeact-GFP mRNA for microinjection for visualization of actin in S. purpuratus

Depositor

Mamiko Yajima

Article

Uchida et al Dev Biol. 2018 Sep 1;441(1):19-30. doi:

Insert

Lifeact-GFP

Use

In vitro transcription (mrna synthesis)

Tags

EGFP

Promoter

Unknown

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pJJS009

Plasmid

#188332

Purpose

PCR template for repair templates to tag a gene with a mKate2 and mIAA7 AID degron using homology-directed repair for C. elegans.

Depositor

Mike Boxem

Article

Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

Insert

mIAA7::mKate2::Linker

Expression

Bacterial

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pJJS003

Plasmid

#188326

Purpose

PCR template for repair templates to tag a gene with a mCherry and mIAA7 AID degron using homology-directed repair for C. elegans.

Depositor

Mike Boxem

Article

Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

Insert

mIAA7::mCherry::Linker

Expression

Bacterial

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-2T_PDZ2

Plasmid

#59735

Purpose

expresses PDZ2 domain of human ZO-1 in bacteria

Depositor

Alan Fanning

Article

Fanning et al J Biol Chem. 2007 Dec 28;282(52):37710-6

Insert

zonula occludens-1 (TJP1 Human)

Tags

GST and thrombin clvg

Expression

Bacterial

Available Since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only
pSEVA43-GG

Plasmid

#208014

Purpose

for cloning homology arms, with GFP as counter selection marker

Depositor

Pablo Ivan Nikel

Article

Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

Insert

msfGFP

Promoter

14G

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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SGL40C.EFS.E2Crimson

Plasmid

#100894

Purpose

Lentiviral gRNA delivery

Depositor

Dirk Heckl

Article

Labuhn et al Nucleic Acids Res. 2018 Feb 16;46(3):137

Insert

E2Crimson

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pDEST26-hOPTN-D474N

Plasmid

#23051

Depositor

Jon Ashwell

Article

Zhu et al Curr Biol. 2007 Aug 21. 17(16):1438-43.

Insert

Optineurin (OPTN Human)

Tags

His

Expression

Mammalian

Mutation

D474N

Available Since

March 16, 2010

Availability

Academic Institutions and Nonprofits only
pUC18T-mini-Tn7T-hph-Ptol

Plasmid

#218369

Purpose

Empty backbone for Tn7-site insertion with XylS-Pm expression system

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available Since

May 2, 2024

Availability

Academic Institutions and Nonprofits only

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pFUS_A3B

Plasmid

#43852

Depositor

Takashi Yamamoto

Article

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

LacZ + BsaI restriction sites

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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pJJS010

Plasmid

#188333

Purpose

PCR template for repair templates to tag a gene with a mKate2 and mIAA7 AID degron using homology-directed repair for C. elegans.

Depositor

Mike Boxem

Article

Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

Insert

Linker::mKate2::mIAA7

Expression

Bacterial

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pJJS004

Plasmid

#188327

Purpose

PCR template for repair templates to tag a gene with a mCherry and mIAA7 AID degron using homology-directed repair for C. elegans.

Depositor

Mike Boxem

Article

Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

Insert

Linker::mCherry::mIAA7

Expression

Bacterial

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pFUS_A4A

Plasmid

#43853

Depositor

Takashi Yamamoto

Article

Sakuma et al Genes Cells. 2013 Feb 6. doi: 10.1111/gt

Insert

LacZ + BsaI restriction sites

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only

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Flag-ECFP-Paxillin

Plasmid

#15211

Depositor

Rick Horwitz

Article

Webb et al J Cell Sci. 2005 Nov 1. 118(Pt 21):4925-

Insert

paxillin (PXN Chicken)

Tags

ECFP and Flag

Expression

Mammalian

Available Since

Sept. 13, 2007

Availability

Academic Institutions and Nonprofits only
pSUPER.PKD2.RNAi

Plasmid

#10816

Depositor

Alex Toker

Article

Storz et al Mol Cell Biol. 2004 Apr . 24(7):2614-26.

Insert

PKD2 RNAi (PRKD2 Human)

Use

RNAi

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only