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pmCherry-3xNLS-OptoJNKi

Plasmid

#89740

Purpose

Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, wild-type photosensor, inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

NLS-OptoJNKi

Use

Fluorescent protein

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pKCAG-mCherry-OptoJNKi

Plasmid

#89738

Purpose

Expression of light-regulated JNK inhibitor, wild-type photosensor, inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

OptoJNKi

Use

Fluorescent protein

Tags

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-NES-OptoJNKi

Plasmid

#89739

Purpose

Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to cytoplasm, wild-type photosensor, inhibitor JIP11

Depositor

Michael Courtney

Article

Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

Insert

NLS-OptoJNKi

Use

Fluorescent protein

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pluc-miR148a-148aT

Plasmid

#65053

Purpose

Reporter plasmid to detect miR148/152 family expression

Depositor

Cristina Fillat

Article

Bofill-De Ros et al Mol Ther. 2014 Sep;22(9):1665-77. doi: 1

Insert

Engineered target sites miR-148a-3p (MIR148A Mouse, Human)

Use

Luciferase

Expression

Mammalian

Promoter

SV40

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pGTM_SUMO_PLpro_W93F_001

Plasmid

#234490

Purpose

Expresses SARS-CoV-2 Nsp3 residues 746-1063 for the purification of PLpro protease with a W93F mutation to allow for 5-fluorotryptophan incorporation and 19F NMR

Depositor

Gaetano Montelione

Article

De Falco et al STAR Protoc. 2025 Sep 19;6(3):103952. do

Insert

PLpro

Tags

6xHis-Sumo

Expression

Bacterial

Promoter

T7 lac

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pGTM_SUMO_PLpro_W106F_001

Plasmid

#234491

Purpose

Expresses SARS-CoV-2 Nsp3 residues 746-1063 for the purification of PLpro protease with a W106F mutation to allow for 5-fluorotryptophan incorporation and 19F NMR

Depositor

Gaetano Montelione

Article

De Falco et al STAR Protoc. 2025 Sep 19;6(3):103952. do

Insert

PLpro (ORF1ab SARS-CoV-2)

Tags

6xHis-Sumo

Expression

Bacterial

Promoter

T7 ac

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pAX1_HS∆P2

Plasmid

#225197

Purpose

Used to create a markerless deletion of the E. coli HS P2 phage

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

P2 phage homology arms

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_HS∆tum

Plasmid

#225198

Purpose

Used to create a markerless deletion of the E. coli HS tum gene

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

tum homology arms

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAX1_HS_gpN:SpyTag

Plasmid

#225193

Purpose

Used for adding C-terminal SpyTag to the E. coli HS P2 phage major capsid protein

Depositor

Travis Wiles

Article

Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

Insert

gpN homology arms and SpyTag

Expression

Bacterial

Available Since

April 18, 2025

Availability

Academic Institutions and Nonprofits only

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pET_4E1H.95

Plasmid

#155213

Purpose

Expresses 4E1H.95 de novo protein in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

4E1H.95

Tags

6x His Tag

Expression

Bacterial

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pET_4H.01

Plasmid

#155210

Purpose

Expresses 4H.01 de novo designed protein in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

4H.01

Tags

6x His Tag

Expression

Bacterial

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET_4E2H.210

Plasmid

#155212

Purpose

Expresses 4E2H.210 de novo protein in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

4E2H.210

Tags

6x His Tag

Expression

Bacterial

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pET_3E2H.37

Plasmid

#155211

Purpose

Expresses 3E2H.37 de novo designed protein in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

3E2H.37

Tags

6x His Tag

Expression

Bacterial

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAc.1B-EGFP-DmHrb98

Plasmid

#66595

Purpose

Expresses EGFP-tagged Hrb98 in Drosophila cells

Depositor

Elisa Izaurralde

Insert

Hrb98 (Hrb98DE Fly)

Use

Drosophila expression

Tags

EGFP

Promoter

Actin 5C

Available Since

June 24, 2015

Availability

Academic Institutions and Nonprofits only

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pBM16

Plasmid

#45344

Depositor

Brendan Cormack

Article

Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86.

Type

Empty backbone

Use

C. glabrata expression

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

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pKT127-SEP

Plasmid

#41899

Depositor

Dan Gottschling

Article

Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

Type

Empty backbone

Tags

superecliptic GFP (SEP)

Expression

Yeast

Available Since

April 17, 2013

Availability

Academic Institutions and Nonprofits only

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pGRB2.1

Plasmid

#45341

Depositor

Brendan Cormack

Article

Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86.

Type

Empty backbone

Use

C. glabrata

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

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pRSF-KpDcr1

Plasmid

#32022

Depositor

David Bartel

Article

Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

Insert

DCR1

Tags

His6-SUMO

Expression

Bacterial

Promoter

T7

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pGRB2.0

Plasmid

#45340

Depositor

Brendan Cormack

Article

Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86.

Type

Empty backbone

Use

C. glabrata

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pBM16.1

Plasmid

#45345

Depositor

Brendan Cormack

Article

Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86.

Type

Empty backbone

Use

C. glabrata

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge