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Plasmid#188788PurposeAvs1 from Erwinia piriflorinigrans CFBP5888DepositorInsertEpAvs1
UseTagsExpressionBacterialMutationPromoterblaAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKP932
Plasmid#167652PurposeExpress colicinB-His6, M261CDepositorInsertcba-his6 M261C
UseTagsExpressionBacterialMutationcba-his6 with M261C mutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWS496
Plasmid#123041PurposeCore promoterDepositorInsertpCYC1m [2b]
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLG005 (Retron-TIR)
Plasmid#157883PurposeExpresses the retron-TIR defense systemDepositorInsertRetron-TIR
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG009 (DRT type 1)
Plasmid#157887PurposeExpresses the DRT type 1 defense systemDepositorInsertDRT type 1 (RT-nitrilase)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG024 (SIR2 + HerA)
Plasmid#157902PurposeExpresses the SIR2 + HerA defense systemDepositorInsertSIR2 + HerA
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG026 (tmn)
Plasmid#157904PurposeExpresses the tmn defense systemDepositorInserttmn (transmembrane ATPase)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG031 (upx)
Plasmid#157909PurposeExpresses the upx defense systemDepositorInsertupx (putative PD-(D/E)xK family phosphodiesterase)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACλCI-β-flap (831-1057)
Plasmid#53733PurposeExpression of λCI fused via three alanines to E. coli RNAP beta subunit, aa 831-1057 (β-flap). Can be cut with Not1 and BamHI to replace β-flap with a moiety of interest.DepositorInsertλCI-β-flap fusion
UseTagsExpressionBacterialMutationPromoterlac UV5Available sinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLG010 (DRT type 2)
Plasmid#157888PurposeExpresses the DRT type 2 defense systemDepositorInsertDRT type 2
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLG033 (ietAS)
Plasmid#157911PurposeExpresses the ietAS defense systemDepositorInsertietAS (ATPase + serine protease)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG034 (restriction-like)
Plasmid#157912PurposeExpresses a restriction-like defense systemDepositorInsert4-gene restriction-like system
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG011 (DRT type 3)
Plasmid#157889PurposeExpresses the DRT type 3 defense systemDepositorInsertDRT type 3 (RT + RT)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWJ66
Plasmid#46570PurposeSame as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)DepositorInsertstracrRNA
dcas9-w
CRISPR array
UseCRISPRTagsrpoZExpressionBacterialMutationD10A, H840APromoterAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLG007 (Ec86 retron)
Plasmid#157885PurposeExpresses the Ec86 retron defense systemDepositorInsertEc86 retron
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG025 (DUF4297 + HerA)
Plasmid#157903PurposeExpresses the DUF4297 + HerA defense systemDepositorInsertDUF4297 + HerA
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG012 (DRT type 4)
Plasmid#157890PurposeExpresses the DRT type 4 defense systemDepositorInsertDRT type 4
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLG013 (DRT type 5)
Plasmid#157891PurposeExpresses the DRT type 5 defense systemDepositorInsertDRT type 5
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG003 (Druantia type I)
Plasmid#157881PurposeExpresses the Druantia type I defense systemDepositorInsertDruantia type I
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG023 (dsr2)
Plasmid#157901PurposeExpresses the dsr2 defense systemDepositorInsertdsr2 (SIR2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits only