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Plasmid#176886PurposeAAV transfer plasmid for cell-surface-localized FRISZ (via PDGFR TM) under hSyn promoterDepositorInsertIg kappa-FRISZ-PDGFR TM
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMK1253
Plasmid#133058PurposeLentiviral vector with EF1A promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-Clover2 fusion protein.DepositorInsertTau.K18(LM)
UseLentiviralTagsmClover2ExpressionMammalianMutationPromoterEF1AAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMK1254
Plasmid#133057PurposeLentiviral vector with EF1A promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-mRuby2 fusion protein.DepositorInsertTau.K18(LM)
UseLentiviralTagsmRuby2ExpressionMammalianMutationPromoterEF1AAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcg 2.0-BFP-circ-arRNA backbone
Plasmid#213965Purposepcg 2.0-BFP-circ-arRNA backbone included a Twister P3 U2A, 5’ ligation sequence, a 3’ ligation sequence and Twister P1 based on pLenti-sgRNA-lib 2.0 backbone (Addgene no. 89638). Then, the sequences oDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-hSyn-FRISZ-GPI
Plasmid#177264PurposeAAV transfer plasmid for cell-surface-localized FRISZ (via CD59 GPI) under hSyn promoterDepositorInsertCD59 leader-FRISZ-GPI
UseAAVTagsCD59 GPI and CD59 leader sequenceExpressionMutationPromoterhSynAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJC49
Plasmid#133060PurposeLentiviral vector with a (Ubiquitous Chromatin Opening Element) UCOE upstream of a CAG promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-mScarlet-I fusion protein.DepositorInsertTau.K18(LM)
UseLentiviralTagsmScarletIExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PEmaxRNaseHtrunc
Plasmid#207858PurposeMammalian expression of PEmaxDRNaseH prime editorDepositorInsertPEmaxDRNaseH
UseTagsSV40 bpNLS and SV40 bpNLS, c-Myc NLSExpressionMammalianMutationM-MLVRTDRNAseHrtPromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE6e
Plasmid#207855PurposeMammalian expression of PE6e prime editorDepositorInsertPE6e
UseTagsSV40 bpNLS and SV40 bpNLS, c-Myc NLSExpressionMammalianMutationCas9(H840A, K918A, K775R)PromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn-fDIO-somQuasAr6a-EGFP-P2A-sombC1C2TG
Plasmid#183537PurposeVoltage indicatorDepositorInsertsomQuasAr6a-EGFP-P2A-sombC1C2TG
UseAAVTagsExpressionMutationNonePromoterhSynAvailable sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
p(HA)HIF1alpha (P402A,P564A)
Plasmid#52636Purposemammalian expression of HA tagged HIF1alpha (full length) with P402A,P564A mutationDepositorInsertHIF1alpha P402A, P564A (HIF1A Human)
UseTagsHAExpressionMammalianMutationP402A, P564APromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.iGluSnFr.WPRE.SV40
Plasmid#98929PurposeAAV expression of glutamate sensor from human synapsin promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas ServiceAAV1, AAV5, and AAV9InsertiGluSnFr
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-DIO eHcKCR1 3.0-oScarlett-KV2.1
Plasmid#195198Purposeall-optical silencing methodDepositorInserteHcKCR1 3.0-oScarlet-Kv2.1
UseAAVTagsExpressionMutationPromoterCaMKIIaAvailable sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-DIO ChRmine-oScarlet-KV 2.1-WPRE
Plasmid#183536PurposeOptogeneticsDepositorInsertChRmine-oScarlet-Kv2.1
UseAAVTagsExpressionMutationNonePromoterCaMKIIaAvailable sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pENN.AAV.GFAP.iGluSnFr.WPRE.SV40
Plasmid#98930PurposeAAV expression of glutamate sensor from GFAP promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas ServiceAAV1, AAV5, and AAV9InsertiGluSnFr
UseAAVTagsExpressionMammalianMutationPromoterGFAPAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.CAG.Flex.iGluSnFr.WPRE.SV40
Plasmid#98932PurposeCre dependent AAV expression of glutamate sensor from GFAP promoter ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas ServiceAAV1, AAV5, and AAV9InsertiGluSnFr
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.Flex.iGluSnFr.WPRE.SV40
Plasmid#98931PurposeCre dependent AAV expression of glutamate sensor from human synapsin promoter. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **DepositorHas ServiceAAV1, AAV5, and AAV9InsertiGluSnFr
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceJan. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pCMV-PE6d
Plasmid#207854PurposeMammalian expression of PE6d prime editorDepositorInsertPE6d
UseTagsSV40 bpNLS and SV40 bpNLS, c-Myc NLSExpressionMammalianMutationM-MLVRTDRNAseHrt(T128N, V223Y, D200C)PromoterAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only