We narrowed to 1,003 results for: transfection
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Plasmid#87199PurposeExpression of ectopic mutated human MRP RNA contstruct from the human RMRP promoter, also contains CMV-puro-t2A-mCherry expression cassette for selection/transfection efficiencyDepositorAvailable SinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only
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pAS_4xG1 PylT sfGFP 150TAG
Plasmid#154767Purposeplasmid with 4xG1 PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
ExpressionMammalianMutation150TAGPromoterEF1Available SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPWCh
Plasmid#124238PurposeGateway destination vector for C-terminal tagging with mCherry. The expression is driven by the UASp promoter. Suitable for Drosophila transformation and transfection.DepositorTypeEmpty backboneExpressionInsectPromoterUASpAvailable SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Multi-Janus-Spacer-1x_pKG1126
Plasmid#246375PurposeMulti-Janus spacer for Galloway Lab Golden Gate cloning scheme. Used as a "filler" plasmid in transfection experiments.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionMammalianMutationnoneAvailable SinceNov. 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
TVMV-HRV-3C Proteases-mCherry
Plasmid#243796PurposeEncodes for mammalian expression of TVMV and HRV-3C protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.DepositorInsertTVMV and HRV-3C proteases
ExpressionMammalianAvailable SinceOct. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
TVMV-TEV Proteases-mCherry
Plasmid#243797PurposeEncodes for mammalian expression of TVMV and TEV protease inputs. Construct contains a mCherry to indicate successful transfection and full plasmid read-through. Each protein is separated by a P2A self-cleaving sequence.DepositorInsertTVMV and TEV proteases
ExpressionMammalianAvailable SinceOct. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBOB-3xFlag-hGpr75
Plasmid#236746PurposeExpress 3xFlag N-terminal tagged wild-type human GPR75 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.DepositorAvailable SinceJuly 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mGL-bGH
Plasmid#235299PurposemGreenLantern expression to serve as a transfection marker, EF1a-drivenDepositorInsertmGreenLantern
UseSynthetic BiologyExpressionMammalianPromoterEF1a (human EF1a)Available SinceMay 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
EFS-mGL-bGH
Plasmid#235255PurposemGreenLantern expression to serve as a transfection marker, EFS-drivenDepositorInsertmGreenLantern
UseSynthetic BiologyExpressionMammalianPromoterEFS (human EF1a short, intronless)Available SinceMay 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBOB-3xFlag-mGpr75
Plasmid#236744PurposeExpress 3xFlag N-terminal tagged wild-type mouse GPR75 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.DepositorAvailable SinceMay 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBOB-3xFlag-mGpr75-L144P
Plasmid#236745PurposeExpress 3xFlag N-terminal tagged mouse GPR75-L144P mutant transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.DepositorAvailable SinceMay 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-tagBFP-bGH
Plasmid#235257PurposetagBFP expression to serve as a "filler" plasmid for transfectionsDepositorInserttagBFP
UseSynthetic BiologyExpressionMammalianMutationSilent mutations to remove BsaI sites in tagBFPPromoterEF1a (human EF1a)Available SinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN_GFP
Plasmid#235611PurposeEncodes GFP. For use in transfections as a negative control without epitope tags.DepositorInsertGFP
ExpressionMammalianAvailable SinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
Venus-Bik-del72-136-pEGFP-C3
Plasmid#191411PurposeTransfection of this plasmid will express "VBik-d72-136" (Venus fused to the N-terminus of the BH3-only protein, Bik that has amino acids 72-136 deleted).DepositorInsertBcl-2-interacting killer (BIK Human)
TagsVenusExpressionMammalianMutationwith deletion of amino acids 72-136 (del72-136) i…PromoterCMVAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tol2-GgVCL/*TBM
Plasmid#162790PurposeMUTATED_transfection of mammalian cells with Vcl mutated in Tankyrase Binding Motif -II (TBM-II) under a β-actin promoter, with IRES-GFP allowing identification of transfected cellsDepositorArticleInsertMutated Gallus gallus vinculin (G454V) (VCL Chicken)
TagsIRES-GFPExpressionBacterial and MammalianMutationchanged Gly 454 to ValPromoterbeta actinAvailable SinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
shh10
Plasmid#64867PurposeThis plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the engineered shh10 capsid protein.DepositorInsertshh10 cap
UseAAVMutationI319V, N451D, D532NAvailable SinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHcRed-NPM1wt-C1
Plasmid#131818PurposeExpresses HcRed-NPM1wildtype (human) in mammalian cells through transient transfectionDepositorAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Flag-NPM1wt
Plasmid#131810PurposeExpresses Flag-NPM1wildtype (human) in mammalian cells through transient transfectionDepositorAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDOE11 Lat52:mCherry transient
Plasmid#124249PurposeAllows bimolecular fluorescence complementation via transient expression of two proteins in directly transfected pollen tubes.DepositorTypeEmpty backboneExpressionPlantAvailable SinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMma PylT sfGPF 150TAG
Plasmid#154766Purposeplasmid with 4xMma PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
ExpressionMammalianMutation150TAG in GFP reporter, U25C in PylTPromoterEF1Available SinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only