We narrowed to 12,495 results for: pcdna

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ciCas9(F22)_pcDNA5

Plasmid

#100552

Purpose

Expresses ciCas9(F22) in mammalian cells. Can be used to generate Flp-In and Flp-In T-REx stables.

Depositor

Dustin Maly

Article

Rose et al Nat Methods. 2017 Jul 24. doi: 10.1038/n

Insert

ciCas9(F22)

Use

CRISPR; Frt, flp-in

Tags

FLAG

Expression

Mammalian

Available Since

Sept. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Fos AA

Plasmid

#8967

Depositor

John Blenis

Article

Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

Insert

c-Fos (Fos Rat)

Tags

FLAG

Expression

Mammalian

Mutation

S362A, S374A

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-sACE2v2-Fc(IgA1)

Plasmid

#145157

Purpose

Mammalian expression plasmid for soluble ACE2-Fc fusion (IgA1) high affinity variant 2

Depositor

Erik Procko

Article

Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

Fc region of human IgA1

Expression

Mammalian

Mutation

Extracellular, soluble protease domain (a.a. M1-D…

Promoter

CMV

Available Since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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1-63 bcr/abl pcDNA3

Plasmid

#27479

Depositor

Warren Pear

Article

He et al Blood. 2002 Apr 15;99(8):2957-68.

Insert

(1-63) bcr/abl (ABL1, BCR Human)

Expression

Mammalian

Mutation

Contains aa 1-63 of bcr and the complete abl chim…

Available Since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only
SUMO3_pcDNA6.2/EmGFP-Bsd

Plasmid

#176949

Purpose

Mammalian expression vector encoding SUMO3 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

SUMO3 (SUMO3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-V5-DGCR8-mDRBD1&2

Plasmid

#51385

Depositor

Narry Kim

Article

Yeom et al Nucleic Acids Res. 2006;34(16):4622-9. E

Insert

DGCR8 (DGCR8 Human)

Tags

V5

Expression

Mammalian

Mutation

DRBD1 & DRBD2 mutant (A568K, A569K, A676K, S6…

Promoter

CMV

Available Since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
pCDNA3.0_LynSNAP IRES EGFP

Plasmid

#136625

Purpose

Plasmid encoding outer inner membrane localization of SNAP-Tag and EGFP

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

Lyn11-SNAP-Tag (IRES EGFP)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CTE-Luc

Plasmid

#60881

Purpose

Reporter construct to study unspliced RNA export. Coding sequence of luciferase gene was sandwiched between 5' and 3' splice site sequences originating from the HIV gag/pol gene followed by CTE

Depositor

Vinay Nandicoori

Article

Rajanala et al PLoS One. 2012;7(1):e29921. doi: 10.1371

Insert

Luc-CTE

Expression

Mammalian

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO DNAJA3

Plasmid

#19466

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJA3 (DNAJA3 Human)

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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Cav2.2 HA D122A pcDNA3

Plasmid

#206108

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a D122A mutation that disrupts the interaction with ?2?-1

Depositor

Annette Dolphin

Article

Dahimene et al Cell Rep. 2018 Nov 6;25(6):1610-1621.e5.

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Mutation

D122A

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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Kohinoor-Tublin/pcDNA3

Plasmid

#67780

Purpose

Tublin-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Tags

b-Tubulin

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA4/TO-UL87-2xStrep

Plasmid

#138434

Purpose

Expresses full-length UL87 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Insert

UL87 (UL87 HCMV (HHV-5))

Tags

Strep

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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Y921F EphA2 pcDNA3

Plasmid

#102736

Purpose

Expression of EphA2 in mammalian cells

Depositor

Jin Chen

Article

Fang et al J Biol Chem. 2008 Jun 6;283(23):16017-26

Insert

EphA2 (Epha2 Mouse)

Expression

Mammalian

Mutation

Y921F (TAC > TTC)

Promoter

CMV

Available Since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-NES-ZapCmR1.1

Plasmid

#59014

Purpose

Cytosolic zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

Insert

NES-ZapCmR1.1 genetically encoded cytosolic Zn(II) sensor

Tags

Nuclear Exclusion Signal

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_mGFP-FGFR3-mScarlet

Plasmid

#192509

Purpose

Expression of N-terminally mGFP and C-terminally mScarlet labelled wildtype FGFR3 (isoform IIIc)

Depositor

Irene Tiemann-Boege

Article

Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi

Insert

mGFP-FGFR3IIIc-mScarlet

Tags

mGFP and mScarlet

Expression

Mammalian

Available Since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA6-MRPS6-VN172

Plasmid

#182374

Purpose

mVenus-based mito-riboBiFC vector

Depositor

Chunghun Lim

Article

Lee et al BMC Biol. 2022 Jan 7;20(1):12. doi: 10.1

Insert

Mrps6 (Mrps6 Mouse)

Tags

VN172

Expression

Mammalian

Promoter

CMV promoter

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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Mouse NCKX6 (pcDNA3.1+)

Plasmid

#75215

Purpose

Mammalian expression of SLC8B1

Depositor

Jonathan Lytton

Article

Cai et al J Biol Chem. 2004 Feb 13;279(7):5867-76.

Insert

SLC8B1 (Slc8b1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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TRAPa-3xFLAG-pCDNA

Plasmid

#201963

Purpose

Mammalian expression of Translocon-associated protein subunit alpha

Depositor

Ville Paavilainen

Article

Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

Insert

SSR1 (SSR1 Human)

Tags

3xFLAG-tag

Expression

Mammalian

Available Since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-p97-AE-mycStrep

Plasmid

#31841

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Tags

Strep and myc

Expression

Bacterial and Mammalian

Mutation

A232E

Promoter

CMV

Available Since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT-TO-Nsp1-HF

Plasmid

#157681

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp1 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

HF-Nsp1 (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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