Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 7,043 results for: plasmid dna

Clear keywords
Showing: 3181 - 3200 of 7043 results

  4. pdCas9-DNMT3A-EGFP

    Plasmid
    #71666
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-EGFP for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-EGFP (DNMT3A S. pyogenes, Synthetic, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-EGFP
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71666-plasmid-map-sequence-id-129527

    Enlarge

  5. pdCas9-DNMT3A-PuroR

    Plasmid
    #71667
    Purpose
    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA
    Depositor
    Vlatka Zoldos
    Article
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Insert
    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A S. pyogenes, Synthetic, Human)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    CBh
    Available Since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    71667-plasmid-map-sequence-id-243369

    Enlarge

  7. LgBiT-KRAS(G12D) Vector

    Plasmid
    #237033
    Purpose
    Express LgBiT-KRAS(G12D) Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    KRAS (KRAS Human)
    Tags
    LgBiT
    Expression
    Mammalian
    Mutation
    G12D
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    237033-plasmid-map-sequence-id-471890

    Enlarge

  8. NanoBRET Assay Vector, NanoLuc-HRAS WT

    Plasmid
    #236866
    Purpose
    Express NanoLuc(R)-HRAS WT in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    HRAS (HRAS Human)
    Tags
    NanoLuc (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236866-plasmid-map-sequence-id-472476

    Enlarge

  9. EPOR-LgBiT/EPOR-SmBiT Bidirectional Vector

    Plasmid
    #238776
    Purpose
    Express EPOR-LgBiT/EPOR-SmBiT Fusion Proteins in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Inserts
    EPOR (EPOR Human)
    EPOR (EPOR Human)
    Tags
    LgBiT and SmBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238776-plasmid-map-sequence-id-479908

    Enlarge

  10. NRF2-SmBiT_MAFG-LgBiT BiBiT Vector

    Plasmid
    #238773
    Purpose
    Express NRF2-SmBiT_MAFG-LgBiT Fusion Protein in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Inserts
    NRF2 (NFE2L2 Human)
    MAFG (MAFG Human)
    Tags
    LgBiT and SmBiT
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    Sept. 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    238773-plasmid-map-sequence-id-479907

    Enlarge

  11. HiBiT-KRAS(G12C) Fusion Vector

    Plasmid
    #236889
    Purpose
    Express HiBiT-KRAS(G12C) in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    KRAS (KRAS Human)
    Tags
    HiBit
    Expression
    Mammalian
    Mutation
    G12C
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236889-plasmid-map-sequence-id-472710

    Enlarge

  12. NanoBRET Assay Vector, HaloTag-KRAS

    Plasmid
    #236871
    Purpose
    Express HaloTag(R)-KRAS in Mammalian Cells under a CMV promoter
    Depositor
    Promega Corporation
    Has Service
    DNA
    Insert
    KRAS (KRAS Human)
    Tags
    HaloTag (R)
    Expression
    Mammalian
    Mutation
    None
    Promoter
    CMV
    Available Since
    July 14, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    236871-plasmid-map-sequence-id-473394

    Enlarge

  13. p189

    Plasmid
    #65207
    Purpose
    Mutant EGFP expression plasmid for the creation of a heteroduplex DNA repair assay plasmid with pGEM5Z(+)-EGFP (p111). *Please see notes in comments section.
    Depositor
    LuZhe Sun
    Article
    Zhou et al Anal Biochem. 2009 May 1;388(1):167-9. d
    Insert
    EGFP
    Expression
    Bacterial and Mammalian
    Mutation
    Premature stop codon added to pGEM5Z(+)-EGFP (TGG…
    Promoter
    CMV
    Available Since
    June 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    65207-plasmid-map-sequence-id-490957

    Enlarge

  14. BM28

    Bacterial Strain
    #201047
    Purpose
    MG1655-derived Escherichia coli isolate obtained from adaptive laboratory evolution to increasing incubation temperatures. Also carries a pBR325 plasmid carrying groESL called pOF39.
    Depositor
    Jeannette Winter
    Article
    McGuire et al BMC Genomics. 2023 Mar 27;24(1):154. doi
    Bacterial Resistance
    Ampicillin and Kanamycin
    Available Since
    May 18, 2023
    Availability
    Academic Institutions and Nonprofits only

  15. TBXTA-c019

    Plasmid
    #139756
    Purpose
    For protein expression
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, WT (TBXT Human)
    Tags
    His6-SUMO
    Expression
    Bacterial
    Mutation
    Contains only amino acids E41-A214
    Promoter
    T7
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139756-plasmid-map-sequence-id-296514

    Enlarge

  16. TBXTA-c020

    Plasmid
    #139757
    Purpose
    For protein expression/crystallography (PDB 5QS0)
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, WT (TBXT Human)
    Tags
    His6-SUMO
    Expression
    Bacterial
    Mutation
    Contains only amino acids E41-N211
    Promoter
    T7
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139757-plasmid-map-sequence-id-296518

    Enlarge

  17. TBXTA-c022

    Plasmid
    #139759
    Purpose
    Protein expression/crystallography
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, G177D (TBXT Human)
    Tags
    His6-SUMO
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids E41-A214
    Promoter
    T7
    Available Since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139759-plasmid-map-sequence-id-296542

    Enlarge

  19. Tet-pLKO-puro

    Plasmid
    #21915
    Purpose
    3rd generation lentiviral plasmid for inducible expression of shRNA; puromycin selection. See manual for detailed protocols. No further technical support is available from the depositing laboratory.
    Depositor
    Dmitri Wiederschain
    Article
    Wiederschain et al Cell Cycle. 2009 Feb 1. 8(3):498-504.
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    Lentiviral
    Available Since
    Aug. 22, 2009
    Availability
    Academic Institutions and Nonprofits only
    21915-plasmid-map-sequence-id-326721

    Enlarge

Showing: 3181 - 3200 of 7043 results