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pET21-pelB-LaRIgG-7

Plasmid

#228997

Purpose

For bacterial expression of anti-rabbit IgG nanobody LaRIgG-7, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaRIgG-7

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaRIgG-1

Plasmid

#228995

Purpose

For bacterial expression of anti-rabbit IgG nanobody LaRIgG-1, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaRIgG-1

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaMIgG-5

Plasmid

#228991

Purpose

For bacterial expression of anti-mouse IgG nanobody LaMIgG-5, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaMIgG-5

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pEN_TT 3xFLAG-CSE1L

Plasmid

#192295

Purpose

Gateway entry vector for an inducible 3xFLAG-CSE1L

Depositor

Kevin Janes

Article

Wang et al Nat Commun. 2023 Apr 13;14(1):2110. doi:

Insert

CSE1L (CSE1L Human)

Use

Gateway entry vector

Tags

3xFLAG

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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G1333 DddAtox-N–SaKKH-Cas9(D10A)

Plasmid

#157837

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

G1333 DddAtox-N–SaKKH-Cas9(D10A)–UGI–SV40 NLS

Expression

Mammalian

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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Myc-CYLD-C601A

Plasmid

#60027

Purpose

Myc-tagged CYLD catalytic inactive (C601A) expression

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2007 May 22. 1

Insert

Cylindromatosis (CYLD Human)

Tags

Myc

Expression

Mammalian

Mutation

Cysteine 601 changed to Alanine

Promoter

CMV

Available Since

Nov. 25, 2014

Availability

Academic Institutions and Nonprofits only

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pATZ-HA-GFP11

Plasmid

#177720

Purpose

ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to an HA tag and the eleventh β-strand of GFP

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

alpha-1-antitrypsin (SERPINA1 Human)

Tags

GFP11 and HA

Expression

Bacterial and Mammalian

Mutation

E342K (ATZ mutant)

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pMX-mTAF4b

Plasmid

#44345

Depositor

Marc Timmers

Article

Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

Insert

TAF4b (Taf4b Mouse)

Use

Retroviral

Tags

flag and SBP

Expression

Mammalian

Promoter

LTR

Available Since

Oct. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-eGFP-P2A-LgBiT-NLS

Plasmid

#187834

Purpose

Expression of eGFP-P2A-LgBiT-NLS from human synapsin promoter via adeno-associated virus for neuronal expression.

Depositor

William McEwan

Article

Tuck et al Cell Rep. 2022 May 3;39(5):110776. doi:

Insert

eGFP-P2A-LgBiT-NLS

Use

AAV

Promoter

hSyn

Available Since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pMX_mTAF3

Plasmid

#44334

Depositor

Marc Timmers

Article

Pijnappel et al Nature. 2013 Mar 28;495(7442):516-9. doi

Insert

TAF3 (Taf3 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Promoter

LTR

Available Since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-human LRRC33

Plasmid

#111602

Purpose

Express human LRRC33 in 293T cells

Depositor

Timothy Springer

Article

Qin et al Cell. 2018 Jun 28;174(1):156-171.e16. do

Insert

human LRRC33 (NRROS Human)

Tags

myc/his tag

Expression

Mammalian

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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RRL.sin.cPPT.CMV/Flag-E2-crimson.IRES-puro.WPRE (MT06)

Plasmid

#139448

Purpose

Lentiviral vector to ectopically express Flag-tagged E2-crimson from CMV promoter

Depositor

Caroline Goujon

Article

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

FLAG-tag E2-crimson

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pEPQD0KN0750

Plasmid

#185627

Purpose

Modified tobacco rattle virus RNA2 acceptor vector that could be used for expression of sgRNAs, contains restriction enzyme sites for golden gate assembly using AarI

Depositor

Nicola Patron

Article

Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

Insert

lacZ

Use

CRISPR and Synthetic Biology

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-YFP-DEPTOR

Plasmid

#73390

Purpose

Expresses HA and YFP tagged DEPTOR

Depositor

Jie Chen, Taekjip Ha

Article

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

DEP Domain Containing MTOR-Interacting Protein (DEPTOR Human)

Tags

HA and YFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
pET21-pelB-LaRIgG-2

Plasmid

#228996

Purpose

For bacterial expression of anti-rabbit IgG nanobody LaRIgG-2, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaRIgG-2

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaGIgG-12

Plasmid

#228999

Purpose

For bacterial expression of anti-mouse IgG nanobody LaMIgG-12, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaGIgG-12

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

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2xCBX7CD_W35A-IFP2.0C

Plasmid

#215755

Purpose

Expresses binding-deficient W35A mutant of 2xCBX7 chromo domain fused to C-terminal part of IFP2.0 as negative control for H3K27me3 detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

2xCBX7CD_W35A-IFP2.0C

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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CamKIIa:smFP-TVA-RGV

Plasmid

#202594

Purpose

Expresses TVA receptor, rabies oG and “spaghetti monster” fluorescent protein in mature excitatory neurons.

Depositor

Oleksandr Shcheglovitov

Article

Chiola et al Mol Psychiatry. 2021 Feb 8. pii: 10.1038

Inserts

smFP
TVA
RGV

Use

Lentiviral

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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NgΔIQ FUNgDIQGW

Plasmid

#92232

Purpose

Lentiviral expression of human Ng with deletion mutation

Depositor

Weifeng Xu

Article

Hwang et al Biol Psychiatry. 2021 Feb 1;89(3):256-26

Insert

Ng (NRGN Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

delta IQ

Promoter

hUbC

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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G1397 DddAtox-C–SaKKH-Cas9(D10A)

Plasmid

#157840

Purpose

expresses split DddAtox-Cas9 construct in mammalian cells

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Insert

G1397 DddAtox-C–SaKKH-Cas9(D10A)–UGI–SV40 NLS

Use

CRISPR

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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