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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,582 results for: eng

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Showing: 3201 - 3220 of 14582 results

  3. pCAG-N2cP

    Plasmid
    #100808
    Purpose
    express rabies virus CVS N2c strain phosphoprotein gene (N2cP)
    Depositor
    Ian Wickersham
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rabies virus CVS N2c strain phosphoprotein gene
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100808-plasmid-map-sequence-id-189510

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  5. pSpdCas9-huTET1CD-T2A-GFP (PX458)

    Plasmid
    #129025
    Purpose
    targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-EGFP and cloning backbone for sgRNA
    Depositor
    Julia K Polansky
    Publication
    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-huTET1CD, SgRNA cloning site
    Tags
    HA-Tag, NLS and T2A-EGFP
    Expression
    Mammalian
    Mutation
    dCas9 (D10A;H840A)
    Promoter
    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)
    Available since
    Jan. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    129025-plasmid-map-sequence-id-454365

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  11. pX335-NEAT1_IS_v2

    Plasmid
    #97086
    Purpose
    Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.
    Depositor
    Archa Fox
    Publication
    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA NEAT1_IS_v2
    Use
    CRISPR
    Promoter
    U6
    Available since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97086-plasmid-map-sequence-id-178717

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  12. pX335-NEAT1_IS_v1

    Plasmid
    #97085
    Purpose
    Encodes an sgRNA that creates a SSB near the poly(A) site of human NEAT1_1 isoform.
    Depositor
    Archa Fox
    Publication
    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA NEAT1_IS_v1
    Use
    CRISPR
    Promoter
    U6
    Available since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    97085-plasmid-map-sequence-id-189638

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  13. pX330-NEAT1m_v1

    Plasmid
    #97083
    Purpose
    Encodes an sgRNA that creates a DSB immediately downstream the poly(A) signal of human NEAT1_1 isoform
    Depositor
    Archa Fox
    Publication
    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA NEAT1m_v1
    Use
    CRISPR
    Promoter
    U6
    Available since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    97083-plasmid-map-sequence-id-189494

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  14. pAAV-CMV-CASANOVA

    Plasmid
    #113036
    Purpose
    AAV vector for CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9
    Depositor
    Dominik Niopek
    Publication
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Insert
    CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
    Use
    AAV, CRISPR, and Synthetic Biology
    Expression
    Mammalian
    Available since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    113036-plasmid-map-sequence-id-226953

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  15. FKBP*-PTEN

    Plasmid
    #86781
    Purpose
    Fusion of PTEN with a modified human FKBP variant harboring the F36V and L106P point mutations (FKBP*) leads to degradation of the fusion protein.
    Depositor
    Britta Eickholt
    Publication
    An et al PLoS One. 2015 Dec 30;10(12):e0145783. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PTEN (PTEN Human)
    Tags
    FKBP* (F36V and L106P) and linker
    Expression
    Mammalian
    Available since
    Feb. 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    86781-plasmid-map-sequence-id-169199

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  17. pS548∙CsR

    Plasmid
    #197859
    Purpose
    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria
    Depositor
    Susanne Häußler
    Publication
    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA scaffold - Tc resistance cassette - incP oriT
    Use
    CRISPR and Synthetic Biology
    Promoter
    PEM7
    Available since
    Oct. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    197859-plasmid-map-sequence-id-393256

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  18. pS648∙CsR

    Plasmid
    #197860
    Purpose
    Expresses Streptococcus pyogenes' cas9 and a tailored sgRNA for counterselection in gram-negative bacteria
    Depositor
    Susanne Häußler
    Publication
    Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA scaffold - Gm resistance cassette - incP oriT
    Use
    CRISPR and Synthetic Biology
    Promoter
    PEM7
    Available since
    Oct. 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    197860-plasmid-map-sequence-id-402537

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Showing: 3201 - 3220 of 14582 results