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pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)-VP64_T2A_MCP-P65-HSF1

Plasmid

#101103

Purpose

Encodes dCas9(C)-VP64 with SAM components fused to VEGFR1

Depositor

Tudor Fulga

Publication

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

VEGFR1-dCas9(C)

Use

CRISPR and Synthetic Biology

Tags

HA

Expression

Mammalian

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pVEGFR2_TEV(N)_NES_TCS(Q'L)_HA-dCas9(N)_P2A_Puro-WPRE

Plasmid

#101104

Purpose

Encodes dCas9(N) fused to VEGFR2

Depositor

Tudor Fulga

Publication

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

VEGFR2-dCas9(N)

Use

CRISPR and Synthetic Biology

Tags

HA

Expression

Mammalian

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_Tiam1-pMagFast2(3x)-iRFP

Plasmid

#67299

Purpose

Blue light dependent photoswitching system

Depositor

Moritoshi Sato

Publication

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Insert

Tiam1-pMagFast2(3x)-iRFP (Tiam1 Mouse, Synthetic)

Expression

Mammalian

Promoter

CMV

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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peCALWY-2

Plasmid

#22234

Depositor

Maarten Merkx

Publication

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eCALWY-2 Zn(II) sensor (ATOX1 Human, GFP's)

Expression

Mammalian

Mutation

sequence identical to eCALWY-1, but linkerlength …

Available since

Nov. 20, 2009

Availability

Academic Institutions and Nonprofits only
SuperClomeleon

Plasmid

#203724

Purpose

An improved engineered fluorescent ratiometric optogenetic sensor to detect inhibitory synaptic activity via changes in intracellular chloride

Depositor

George Augustine

Publication

Grimley et al J Neurosci. 2013 Oct 9;33(41):16297-309.

Insert

Cerulean linked topaz fluorescent protein

Expression

Bacterial and Mammalian

Mutation

Cerulean, modified Topaz Fluorescent Protein with…

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-PHP.eC

Plasmid

#201903

Purpose

non-standard AAV2 rep-AAV-PHP.eC cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Publication

Shay et al Sci Adv. 2023 Apr 21;9(16):eadg6618. doi

Insert

Synthetic construct isolate AAV-PHP.eC VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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pSLIK-Rbfox2-rABE-Flag

Plasmid

#191384

Purpose

Doxycycline inducible expression of Rbfox2-rABE

Depositor

Stephen Floor

Publication

Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

Insert

Rbfox2-rABE (RBFOX2 Human, Synthetic)

Use

Lentiviral

Tags

Flag

Promoter

tet-inducible promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2 sgNRF2-4

Plasmid

#186839

Purpose

knock out NRF2 in mammalian cells

Depositor

Boyi Gan

Publication

Koppula et al Nat Commun. 2022 Apr 22;13(1):2206. doi:

Insert

Nrf2 (Nuclear factor-erythroid factor 2-related factor 2) (NFE2L2 Human)

Use

CRISPR and Lentiviral

Available since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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(133) pcDNA3.1-Nup62-Flag-EPEA

Plasmid

#160744

Purpose

Expresses the nucleoporin Nup62 with C-terminal Flag and EPEA tags. Proteins of interest can be inserted by replacing Nup62 using Sgfl + Sgsl restriction enzymes

Depositor

Christina Woo

Publication

Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

Insert

Nup62-Flag-EPEA

Tags

EPEA and Flag

Expression

Mammalian

Promoter

T7/CMV

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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FRB_LD 0_CS G_tTA-BFP

Plasmid

#58876

Purpose

MESA target chain with Rapamycin FRB ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

Depositor

Joshua Leonard

Publication

Daringer et al ACS Synth Biol. 2014 Mar 11.

Insert

MESA target chain with FRB ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence

Use

AAV

Tags

EBFP

Expression

Mammalian

Promoter

CMV

Available since

Nov. 10, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSimpleII-U6-tracr-U6-crRNA(tdTomato)-NLS-NmCas9-HA-NLS(s)

Plasmid

#47869

Purpose

All-in-one plasmid targeting tdTomato

Depositor

Erik Sontheimer, James Thomson

Publication

Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

Inserts

NmCas9
U6pr-tracrRNA
tdTomato crRNA

Use

CRISPR

Tags

HA and NLS

Expression

Mammalian

Available since

Sept. 30, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTRANS_213

Plasmid

#91112

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: WDV

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pCgsgRNA_crtYf

Plasmid

#153338

Purpose

Double-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; rep oriVC. glutamicum; pMB1 oriVE. Coli; Pj23119-crRNA targeting crtYf; Spcr

Depositor

Sheng Yang

Publication

Zhang et al ACS Synth Biol. 2020 Jul 17;9(7):1897-19

Insert

crRNA targeting crtYf

Use

CRISPR

Expression

Bacterial

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pET51b(+)_H-Luc

Plasmid

#113924

Purpose

Plasmid encoding the bacterial expression of the chimeric HaloTag7-NanoLucCP fusion dubbed H-Luc, for BRET

Depositor

Kai Johnsson

Publication

Hiblot et al Angew Chem Int Ed Engl. 2017 Nov 13;56(4

Insert

H-Luc

Use

Luciferase

Expression

Bacterial

Promoter

T7

Available since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pKUb-Tol2

Plasmid

#85601

Purpose

Source of Tol2 transposase driven by a human ubiquitin promoter.

Depositor

Karl Clark, Scott Fahrenkrug

Publication

Clark et al BMC Biotechnol. 2007 Jul 17;7:42.

Insert

Tol2 transposase

Expression

Mammalian

Promoter

human Ubiquitin

Available since

Feb. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pRG206

Plasmid

#64527

Purpose

Integrative E. coli/S. cerevisiae shuttle vector

Depositor

Joerg Stelling

Publication

Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

Type

Empty backbone

Expression

Yeast

Available since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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mPapaya1-C1

Plasmid

#54722

Purpose

Localization: C1 Cloning Vector, Excitation: 530, Emission: 541

Depositor

Robert Campbell, Michael Davidson

Publication

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Type

Empty backbone

Tags

mPapaya1

Expression

Mammalian

Available since

June 20, 2014

Availability

Academic Institutions and Nonprofits only
pXP842

Plasmid

#46068

Depositor

Nancy DaSilva

Publication

Shen et al Yeast. 2012 Dec;29(12):495-503. doi: 10.

Type

Empty backbone

Expression

Yeast

Promoter

ADH2

Available since

Sept. 10, 2013

Availability

Academic Institutions and Nonprofits only

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pES001 MBP-GSK3β-HA-His

Plasmid

#154010

Purpose

Expresses MBP-GSK3β-HA-His (human Glycogen Synthase Kinase 3β as a fusion protein with MBP, HA, and His- tags) in E.coli

Depositor

Jesse Zalatan

Publication

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

GSK3B (GSK3B Human)

Tags

HA tag, His6, and MBP

Expression

Bacterial

Promoter

Tac

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-P4:gs:nls:VPR

Plasmid

#135983

Purpose

Expresses the P4 coiled coil fused to the VPR transcriptional activation domain in mammalian cells

Depositor

Roman Jerala

Publication

Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

Insert

P4 coiled-coil fused to the VPR activation domain

Expression

Mammalian

Promoter

CMV

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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