We narrowed to 9,350 results for: pho;

1. p21-M694V-MEFV

   Plasmid

   #134703

   Purpose

   Lentiviral expression of human MEFV M694V after doxycycline administration

   Depositor

   Thomas Henry

   Article

   Magnotti et al EMBO Mol Med. 2019 Oct 7:e10547. doi: 10

   Insert

   MEFV (MEFV Human)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mutation

   p.M694V

   Promoter

   TRE2 (Dox-inducible)

   Available since

   Dec. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-UbcH7(C86K)-10aa-linker-delta 1-29, 117-160 and 412-496 Bactrocera parkin

   Plasmid

   #128384

   Purpose

   expresses protein UbcH7 (C86K) fused to Bactrocera parkin with residues 1-29, 117-160 and 412-496 deleted

   Depositor

   Kalle Gehring

   Article

   Sauve et al Nat Struct Mol Biol. 2018 Jul;25(7):623-

   Insert

   UbcH7 (UBE2L3 Human)
   

   Use

   Tags

   Bactrocera dorsalis parkin deleted residues 1 to …

   Expression

   Bacterial

   Mutation

   mutation C86K in UbcH7 gene

   Promoter

   lac promotor

   Available since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3_shRNA resistant nmPKA-Cα-mEGFP

   Plasmid

   #114144

   Purpose

   The G2A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.

   Depositor

   Haining Zhong

   Article

   Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

   Insert

   mouse PKA Cα-mEGFP with G2A mutation and silent mutation to resist shRNA knockdown (Prkaca Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G2A mutation and silent mutation to resist shRNA …

   Promoter

   Available since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. MSCV-Puro-FH-AGO2-S824E-S828E

   Plasmid

   #91999

   Purpose

   Expresses FLAG-HA-AGO2 (S824E-S828E)

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   AGO2 (AGO2 Human)
   

   Use

   Retroviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   S824E, S828E

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSCV-Puro-FH-AGO2-S824E-S831E

   Plasmid

   #92000

   Purpose

   Expresses FLAG-HA-AGO2 (S824E-S831E)

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   AGO2 (AGO2 Human)
   

   Use

   Retroviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   S824E, S831E

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCV-Puro-FH-AGO2-S828E-S831E

   Plasmid

   #92001

   Purpose

   Expresses FLAG-HA-AGO2 (S828E-S831E)

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   AGO2 (AGO2 Human)
   

   Use

   Retroviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   S828E, S831E

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSCV-Puro-FH-AGO2-S824E-S828E-S831E

   Plasmid

   #92002

   Purpose

   Expresses FLAG-HA-AGO2 (S824E-S828E-S831E)

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   AGO2 (AGO2 Human)
   

   Use

   Retroviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   S824E, S828E, S831E

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSCV-Puro-FH-AGO2-S824A-T830A

   Plasmid

   #92003

   Purpose

   Expresses FLAG-HA-AGO2 (S824A-T830A)

   Depositor

   Joshua Mendell

   Article

   Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

   Insert

   AGO2 (AGO2 Human)
   

   Use

   Retroviral

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   S824A, T830A

   Promoter

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-Flag-NSF/T646A

   Plasmid

   #74937

   Purpose

   Mammalian expression of human NSF mutant T646A (mutation in the D2 domain of the protein)

   Depositor

   Elisa Greggio

   Article

   Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

   Insert

   NSF (NSF Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   T646A (mutation in the D2 domain of the protein)

   Promoter

   CMV

   Available since

   April 26, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3-Flag-NSF/T645A

    Plasmid

    #74936

    Purpose

    Mammalian expression of human NSF mutant T645A (mutation in the D2 domain of the protein)

    Depositor

    Elisa Greggio

    Article

    Belluzzi et al Mol Neurodegener. 2016 Jan 13;11(1):1. d

    Insert

    NSF (NSF Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    T645A (mutation in the D2 domain of the protein)

    Promoter

    CMV

    Available since

    April 20, 2016

    Availability

    Academic Institutions and Nonprofits only

11. Progerin-S22D-CS-pLPC

    Plasmid

    #73810

    Purpose

    Progerin mutant where Serine 22 is mutated to aspartic acid and where there is a substitution of cysteine in CaaX-motif by a serine

    Depositor

    Gerardo Ferbeyre

    Article

    Moiseeva et al Aging (Albany NY). 2016 Feb;8(2):366-81.

    Insert

    Progerin (LMNA Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Progerin with substitution of cysteine in CaaX-mo…

    Promoter

    CMV

    Available since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pCAGGS-CD4-YEML-myc

    Plasmid

    #58538

    Purpose

    Expresses human CD4 with the IFITM3 endocytosis motif (YEML) and a C-terminal myc tag in mammalian cells.

    Depositor

    Jacob Yount

    Article

    Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

    Insert

    CD4 (CD4 Human)
    

    Use

    Tags

    IFITM3 endocytosis Tyrosine-Glutamate-Methionine-…

    Expression

    Mammalian

    Mutation

    Added C-terminal Tyrosine-Glutamate-Methionine-Le…

    Promoter

    Available since

    Sept. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pGL4-AARE-luc2P-Hygro

    Plasmid

    #101787

    Purpose

    Luferase reporter plasmid containing three tandem repeats of the amino acid response element (AARE)

    Depositor

    Seiichi Oyadomari

    Article

    Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

    Insert

    3xAARE
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    minimal TATA-box promoter with low basal activity

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-His6-ERK2-MEK1_R4F_coexpression

    Plasmid

    #39212

    Depositor

    Melanie Cobb

    Article

    Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

    Inserts

    Mek1 R4F (MAP2K1 Human)
    Erk2 (Mapk1 Rat)
    

    Use

    Tags

    His6 and RBS

    Expression

    Bacterial

    Mutation

    MEK1(R4F) = S218E, S222D and deletion of AA32-51

    Promoter

    T7 and T7 via RBS

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX SHP-2(NC)-SH2

    Plasmid

    #46499

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SHP-2(NC) (PTPN11 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1-222

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA 3.2/V5-DEST CDC42EP1 S192A

    Plasmid

    #69820

    Purpose

    Expresses CDC42EP1-V5 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    CDC42 effector protein (CDC42EP1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    S192A

    Promoter

    CMV

    Available since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST26 Ago2

    Plasmid

    #59904

    Purpose

    Expresses 6xHis-Ago2 (N-termi tag) in mammalian cells

    Depositor

    Carl Novina

    Article

    Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

    Insert

    Argonaute 2 (AGO2 Human)
    

    Use

    Tags

    6xHis (N-termi tag)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-ARAP3

    Plasmid

    #39484

    Depositor

    Sonja Vermeren

    Article

    Krugmann et al Mol Cell. 2002 Jan;9(1):95-108.

    Insert

    ARAP3 (ARAP3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A

    Plasmid

    #51248

    Purpose

    Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907,980,1006 mutated to Ala

    Depositor

    Rajat Rohatgi

    Article

    Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

    Insert

    Gli3 (Gli3 Mouse)
    

    Use

    Flpin system

    Tags

    3xHA-TEV and Flag

    Expression

    Mammalian

    Mutation

    amino acids 849,865,877,907,980,1006 mutated to A…

    Promoter

    EF1a

    Available since

    March 7, 2014

    Availability

    Academic Institutions and Nonprofits only

20. GFP-ING2(pHD)

    Plasmid

    #21589

    Depositor

    Junying Yuan

    Article

    Gozani et al Cell. 2003 Jul 11. 114(1):99-111.

    Insert

    GFP-ING2(pHD) (Ing2 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    contains Ing2 plant homeodomain (PHD) amino acids…

    Promoter

    Available since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only