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pVENUS-N1-POLR2G-GFP

Plasmid

#86872

Purpose

To express POLR2G (RPB7) tagged with VENUS at C terminus.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

POLR2G (POLR2G Human)

Tags

VENUS

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pI1-MTU-DO-G418

Plasmid

#160012

Purpose

Integrative vector for K. marxianus, targeting the LAC4 locus. G418 selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pICSL12006

Plasmid

#50270

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter + 5'UTR (Cassava Vein Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pTHE

Plasmid

#12512

Depositor

Tong-Chuan He

Article

Jiang et al J Biol Chem. 2001 Nov 30. 276(48):45168-

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 13, 2006

Availability

Academic Institutions and Nonprofits only

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pICSL80005

Plasmid

#50322

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, variant of turboGFP codon-optimised for plants (Pontellina plumata)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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J135F_BattB_Vif(T27X4)-IRES-SpyTag-sfmCherry2(11)-PuroR

Plasmid

#200637

Purpose

Recombination plasmid for Matreyek Bxb1(GT) landing pad in dual landing pad cells

Depositor

Kenneth Matreyek

Article

Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

Insert

Vif

Expression

Mammalian

Mutation

T27stop

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pJ211_WT_low

Plasmid

#128848

Purpose

Low copy number plasmid for expression of E. coli flagellin from its natural promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pET26-Ufd1-His

Plasmid

#21266

Depositor

Hemmo Meyer

Article

Bruderer et al J Biol Chem. 2004 Nov 26. 279(48):49609-

Insert

Ufd1 (Ufd1l Mouse)

Tags

His

Expression

Bacterial

Available Since

July 1, 2009

Availability

Academic Institutions and Nonprofits only

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pAMP-CY1_NF1 T1 (+minintr, KDR)

Plasmid

#119200

Purpose

transfer vector

Depositor

Helen Morrison

Article

Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

Insert

NF1 type 1 mini-gene (NF1 Human)

Use

Transfer vector

Available Since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pI3-MTU-DO-G418

Plasmid

#160023

Purpose

Integrative vector for K. marxianus, targeting integration site I3 described in (Rajkumar et al.,2019). G418 selection.

Depositor

John Morrissey

Article

Rajkumar et al Microb Cell Fact. 2020 Nov 11;19(1):207.

Type

Empty backbone

Use

Kluyveromyces marxianus

Expression

Yeast

Promoter

n/a

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pFW11-ffluc

Plasmid

#128487

Purpose

Allelic exchange plasmid to to integrate firefly luciferase gene into S. pyogenes for biophotonic imaging

Depositor

Thomas Proft

Article

Loh et al Appl Microbiol Biotechnol. 2013 Nov;97(2

Insert

firefly luciferase gene

Use

Allelic replacement vector

Tags

spy0535 downstream flanking region and spy0535 up…

Promoter

synthetic

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCbeta EV (PKA catalytic subunit Cbeta)

Plasmid

#15311

Depositor

Stanley McKnight

Article

Uhler et al J Biol Chem. 1987 Nov 5. 262(31):15202-7

Insert

PRKACB (Prkacb Mouse)

Expression

Mammalian

Available Since

July 6, 2007

Availability

Academic Institutions and Nonprofits only
pDEST_6xUAS:miRFP670-cetn4

Plasmid

#192357

Purpose

zfCETN4 tagged with miRFP670, expressed under the UAS promoter

Depositor

Darren Gilmour

Article

Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

Insert

CETN4 (cetn4 Zebrafish)

Tags

miRFP670

Expression

Mammalian

Available Since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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mt TEFM

Plasmid

#205058

Purpose

TEFM protein expression

Depositor

Smita Patel

Article

Sultana et al J Biol Chem. 2017 Nov 3;292(44):18145-18

Insert

TEFM (TEFM Human)

Expression

Bacterial

Mutation

Codon optimized

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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mVenus-PA-MKI

Plasmid

#65457

Purpose

photoactivatable myosin light-chain kinase inihibition

Depositor

Klaus Hahn

Article

Yi et al ACS Synth Biol. 2014 Nov 21;3(11):788-95

Insert

MKI

Expression

Mammalian

Promoter

CMV

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only
pBYK01

Plasmid

#138656

Purpose

phiOZJ integrating Streptomyces expression vector lacking RDF

Depositor

Joshua Blodgett

Article

Ko et al J Ind Microbiol Biotechnol. 2019 Nov 8.

Type

Empty backbone

Expression

Bacterial

Promoter

PermE* variant sequence from pDA1652

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pTORGm43GWR

Plasmid

#112905

Purpose

Multisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of tdTomato.

Depositor

Sebastian Schornack

Article

Evangelisti et al BMC Microbiol. 2019 Nov 27;19(1):265. do

Type

Empty backbone

Use

Oomycete expression

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET28C-tCorn

Plasmid

#124588

Purpose

Bacterial Expression of tRNA-Corn aptamer

Depositor

Samie Jaffrey

Article

Song et al Nat Chem Biol. 2017 Nov;13(11):1187-1194

Insert

tRNA-Corn

Expression

Bacterial

Promoter

lacI

Available Since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pMito-GBPen

Plasmid

#128269

Purpose

Expression of mitochondrially targeted GBPen (GFP Nanobody)

Depositor

Stephen Royle

Article

Kuey et al J Cell Sci. 2019 Nov 6;132(21). pii: jcs

Insert

Mito-GBPen

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pZZMP122

Plasmid

#231756

Purpose

Mammalian expression of SPO11-YF

Depositor

Scott Keeney

Article

Zheng et al bioRxiv [Preprint]. 2024 Nov 21:2024.11.

Insert

SPO11-YF (Spo11 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Y138F

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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