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pDUAL-P41nmt1-Nup124-GFP-MTn

Plasmid

#154365

Purpose

Yeast expression of Nup124

Depositor

Wanzhong He

Article

Jiang et al Nat Methods. 2020 Sep;17(9):937-946. doi

Insert

Nup124 (nup124 Fission Yeast)

Expression

Yeast

Mutation

See depositor comments below

Available Since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pUT1109-HQ858666

Plasmid

#27719

Depositor

John Gray, Erich Grotewold

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

C2C2-CO-like transcription factor from DT643534

Use

Entry vector

Available Since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only

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pCHKU34.1-2.2

Plasmid

#115534

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

PKS

Expression

Yeast

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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pBT2-4xUAS:P2A-mRFP

Plasmid

#127593

Purpose

This vector is used for cloning a gene driven by 4xnrUAS, to be co-expressed with mRFP through P2A self-cleaving peptides.

Depositor

Longhou Fang

Article

Gu et al Science. 2019 Mar 8;363(6431):1085-1088.

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pUT1141-HQ858685

Plasmid

#27731

Depositor

John Gray, Erich Grotewold

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

bZIP transcription factor from EE179215

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV-Flag-YAP-H122L

Plasmid

#33086

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 )

Tags

Flag

Expression

Mammalian

Mutation

H122L

Promoter

CMV

Available Since

March 21, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-P129D

Plasmid

#33084

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

P129D

Promoter

CMV

Available Since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only
pUT1439-HQ858741

Plasmid

#27823

Depositor

John Gray, Erich Grotewold

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

NAC transcription factor from DT947867

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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pUT1769-HQ858783

Plasmid

#27865

Depositor

John Gray, Erich Grotewold

Article

Burdo et al Plant J. 2014 Oct;80(2):356-66. doi: 10.

Insert

DBP transcription factor from EB701836

Use

Entry vector

Available Since

March 25, 2011

Availability

Academic Institutions and Nonprofits only

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pCDH-CMV-RFP-YAP (isoform 5)

Plasmid

#235690

Purpose

Express RFP tagged YAP gene in mammalian cells using the CMV promoter

Depositor

Duojia Pan

Article

Wang et al Cell. 2022 Nov 10;185(23):4376-4393.e18.

Insert

YAP (YAP1 Human)

Use

Lentiviral

Tags

RFP

Expression

Mammalian

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pRDA355_sgCiPOLR2D

Plasmid

#229022

Purpose

Expression of a CRISPRi doxycycline inducible guide targeting POLR2D

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

POLR2D gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pRDA052H_sgCh2

Plasmid

#229023

Purpose

Expression of a EnAsCas12a control guide that cuts an intergenic region on chromosome 2

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

sgChr2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRDA052H_sgFOCAD #2

Plasmid

#229024

Purpose

Expression of a EnAsCas12a guide targeting FOCAD

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Insert

FOCAD gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRDA_052_HygroR

Plasmid

#228937

Purpose

Plasmid for cloning of custom gRNA using EnAsCas12a

Depositor

Francisca Vazquez

Article

Borck et al Nature. 2025 Feb 5. doi: 10.1038/s41586-

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-MYL2-HA-TadA8e-Sauri N aa1-438-inteinN-U6-Camk2d sgRNA7

Plasmid

#226678

Purpose

Expresses TadA8e and Sauri cas9N by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, TadA, nSauri Cas9N, inteinN

Use

AAV

Promoter

MYL2

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAdEasy-hALDOA K230A

Plasmid

#210814

Purpose

To block the formation of the initial Schiff base with FBP

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOA (ALDOA Human)

Use

Adenoviral

Tags

HA

Mutation

residues 230 of ALDOA mutated to alanine

Promoter

CMV

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-hALDOA K230A

Plasmid

#210811

Purpose

To block the formation of the initial Schiff base with FBP

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOA (ALDOA Human)

Tags

HA

Expression

Mammalian

Mutation

residues 230 of ALDOA mutated to alanine

Promoter

CMV

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pBoBi-hALDOA K230A

Plasmid

#210808

Purpose

To block the formation of the initial Schiff base with FBP

Depositor

Sheng-cai Lin

Article

Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

Insert

ALDOA (ALDOA Human)

Use

Lentiviral

Tags

HA

Mutation

residues 230 of ALDOA mutated to alanine

Promoter

CMV

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pnCas9-miniSdd3-PBE

Plasmid

#204857

Purpose

For cytosine base editing using miniSdd3 in monocotyledon plants (transient transformation)

Depositor

Caixia Gao

Article

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

miniSdd3-nSpCas9(D10A)-UGI

Use

CRISPR

Expression

Plant

Mutation

D10A in SpCas9

Promoter

ZmUbi-1

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-TALE-R-JAK2-Ddd9-C

Plasmid

#204854

Purpose

For DdCBE editing using Ddd9 at JAK2 site in human cells

Depositor

Caixia Gao

Article

Huang et al Cell. 2023 Jul 20;186(15):3182-3195.e14.

Insert

Ddd9-C

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2023

Availability

Academic Institutions and Nonprofits only

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