We narrowed to 17,911 results for: rev

1. RCP83

   Plasmid

   #163466

   Purpose

   Backbone plasmid for insertion of the Saccharomyces cerevisiae promoter library and yeGFP

   Depositor

   Frank Albert

   Article

   Renganaath et al Elife. 2020 Nov 12;9. pii: 62669. doi: 1

   Inserts

   Promoter fragment (Sharon2012_1922)
   His3 minimal promoter
   yeGFP
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pst39-NST-clone14

   Plasmid

   #133421

   Purpose

   S. cerevisiae Nip1 (with C-terminal TEV cleavage site and strep-II tag), Sui1 and Tif5B6 (with N-terminal 7xHis tag and TEV cleavage set) coding sequences with intervening ribosome binding sites and other cis-elements for expression as a polycistron, T7 theta 10 promoter (and corresponding terminator after polycistron).

   Depositor

   Gerhard Wagner

   Insert

   NIP1/SUI1/TIF5 (NIP1, TIF5, SUI1 Budding Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   ORF for Nip1 encodes aa 4-156 (not complete ORF),…

   Promoter

   Available since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pILGFPB6

   Plasmid

   #83557

   Purpose

   Used to evaluate the expression output of GAL10 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.

   Insert

   GAL10 promoter-yEGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL10

   Available since

   Dec. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGMCKq20-T38S38-0X

   Plasmid

   #49522

   Purpose

   Kanamycin resistant; contains qtag20 and constitutively expresses reverse TetR (tetR38); integrates into the mycobacteriophage att-L5 site

   Depositor

   Dirk Schnappinger

   Article

   Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

   Insert

   constitutive TetR38
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptb38

   Available since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. Dom34(1-377)

   Plasmid

   #19100

   Depositor

   SeWon Suh

   Article

   Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

   Insert

   Protein DOM34 (DOM34 Budding Yeast)
   

   Use

   Tags

   C tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 31, 2008

   Availability

   Academic Institutions and Nonprofits only

6. Dom34 E23K

   Plasmid

   #19105

   Depositor

   SeWon Suh

   Article

   Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

   Insert

   Protein DOM34 (DOM34 Budding Yeast)
   

   Use

   Tags

   C tag

   Expression

   Bacterial

   Mutation

   E23K

   Promoter

   Available since

   Dec. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

7. Dom34 E149K

   Plasmid

   #19107

   Depositor

   SeWon Suh

   Article

   Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

   Insert

   Protein DOM34 (DOM34 Budding Yeast)
   

   Use

   Tags

   C tag

   Expression

   Bacterial

   Mutation

   E149K

   Promoter

   Available since

   Dec. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

8. Dom34 F218A

   Plasmid

   #19108

   Depositor

   SeWon Suh

   Article

   Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

   Insert

   Protein DOM34 (DOM34 Budding Yeast)
   

   Use

   Tags

   C tag

   Expression

   Bacterial

   Mutation

   F218A

   Promoter

   Available since

   Dec. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

9. Dom34 E261K

   Plasmid

   #19109

   Depositor

   SeWon Suh

   Article

   Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

   Insert

   Protein DOM34 (DOM34 Budding Yeast)
   

   Use

   Tags

   C tag

   Expression

   Bacterial

   Mutation

   E261K

   Promoter

   Available since

   Dec. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

10. Dom34 Y300A

    Plasmid

    #19110

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Protein DOM34 (DOM34 Budding Yeast)
    

    Use

    Tags

    C tag

    Expression

    Bacterial

    Mutation

    Y300A

    Promoter

    Available since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

11. Dom34 K45A

    Plasmid

    #19111

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Protein DOM34 (DOM34 Budding Yeast)
    

    Use

    Tags

    C tag

    Expression

    Bacterial

    Mutation

    K45A

    Promoter

    Available since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

12. Pdest-EF1α-EGFP-32bp barcode polyA library

    Pooled Library

    #211610

    Purpose

    Barcoded retrovirus plasmid library for capturing the transcriptome and viral barcode sequences of individual cells to infer their identities and lineage information.

    Depositor

    Yuejun Chen

    Article

    You et al Cell Stem Cell. 2023 Apr 6;30(4):473-487

    Species

    Synthetic

    Use

    Retroviral

    Available since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

13. pMW41: pET-His-MBP-SUMO-R2Bm-SII

    Plasmid

    #209290

    Purpose

    Expresses the R2Bm ORF in E. coli

    Depositor

    Feng Zhang

    Article

    Wilkinson et al Science. 2023 Apr 21;380(6642):301-308.

    Insert

    R2
    

    Use

    Tags

    His, MBP, StrepII, and bdSUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC57-LbNOX

    Plasmid

    #75285

    Purpose

    This plasmid contains human codon optimized sequence of LbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.

    Depositor

    Vamsi Mootha

    Article

    Titov et al Science. 2016 Apr 8;352(6282):231-5. doi

    Insert

    LbNOX
    

    Use

    Tags

    C-terminal FLAG tag with linker

    Expression

    Mutation

    Human codon optimized

    Promoter

    Available since

    May 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-6xHis-SUMO-MarathonRT

    Plasmid

    #109029

    Purpose

    Group II intron maturase from Eubacterium rectale (wild-type sequence). This is a highly processive reverse transcriptase that is called “MarathonRT”

    Depositor

    Anna Pyle

    Article

    Zhao et al RNA. 2018 Feb;24(2):183-195. doi: 10.126

    Insert

    ERE_01490
    

    Use

    Tags

    6xHis-SUMO tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pILGFPB5A

    Plasmid

    #83556

    Purpose

    Used to evaluate the expression output of GAL1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    GAL1 promoter-yEGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pZS157 CRISPEY RT/Cas9

    Plasmid

    #114454

    Purpose

    Yeast Integration Plasmid for galactose inducible Ec86-RT and SpCas9 expression

    Depositor

    Hunter Fraser

    Article

    Sharon et al Cell. 2018 Sep 18. pii: S0092-8674(18)31

    Insert

    SpCas9, Ec86-RT
    

    Use

    CRISPR

    Tags

    SV40-NLS

    Expression

    Yeast

    Mutation

    S. cerevisiae codon optimized

    Promoter

    GAL1-GAL10

    Available since

    Oct. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLOX-TERT-iresTK

    Plasmid

    #12245

    Purpose

    2nd generation lentiviral vector for reversible immortalization of human primary cells

    Depositor

    Didier Trono

    Article

    Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

    Insert

    TERT, HSV1-TK (TERT Human)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    LoxP in 3' LTR.

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRCC-K

    Plasmid

    #81191

    Purpose

    Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below. and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available since

    Sept. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pREDTAI

    Plasmid

    #51627

    Purpose

    E coli Genetic Modification

    Depositor

    Sheng Yang

    Article

    Yang et al Appl Environ Microbiol. 2014 Jul 1;80(13

    Insert

    I-SceI (SCEI Budding Yeast)
    

    Use

    Red recombinase and i-scei endonuclease

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    May 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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