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pAAV-CAG-Lck-mScarlet3

Plasmid

#234540

Purpose

to label the membrane of cells with a fluorescent protein (mScarlet3)

Depositor

Hajime Hirase

Publication

Wang et al Anat Sci Int. 2025 Jun 27. doi: 10.1007/

Insert

Lck-mScarlet3

Use

AAV

Mutation

None

Promoter

Short CAG

Available since

June 11, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_AIO-TetOn_Divergent Donor

Plasmid

#229786

Purpose

Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)

Depositor

Richard Davis

Publication

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

LacZ; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE and CAG

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT_AIO-TetOn_Down-Tandem Donor

Plasmid

#229783

Purpose

Bxb1-GT donor plasmid with downstream tandem syntax for all-in-one doxycycline-inducible expression (empty)

Depositor

Richard Davis

Publication

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

LacZ; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE and CAG

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GT_CAG-mRuby2

Plasmid

#229801

Purpose

Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of mRuby2

Depositor

Richard Davis

Publication

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_CAG-EGFP

Plasmid

#229799

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of EGFP

Depositor

Richard Davis

Publication

Blanch-Asensio et al Nat Biomed Eng. 2026 Apr 30. doi: 10.103

Insert

EGFP

Use

Synthetic Biology

Expression

Mammalian

Promoter

CAG

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pTBL3478 PB-PEmax-mTagBFP2

Plasmid

#226664

Purpose

PiggyBac cargo vector encoding PEmax and mTagBFP2

Depositor

Chang Liu

Publication

Loveless et al Nat Chem Biol. 2025 Apr;21(4):512-521. d

Inserts

PEmax
mTagBFP2

Use

CRISPR

Expression

Mammalian

Promoter

CAG and UBC

Available since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG2-EGFP-C-IFT122

Plasmid

#218737

Purpose

Expresses C-terminally EGFP-tagged IFT122 in mammalian cells

Depositor

Kazuhisa Nakayama

Insert

IFT122 (IFT122 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG2-EGFP-C-IFT139

Plasmid

#218738

Purpose

Expresses C-terminally EGFP-tagged IFT139 in mammalian cells

Depositor

Kazuhisa Nakayama

Insert

IFT139 (TTC21B Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-EGFP-N-IFT80

Plasmid

#218731

Purpose

Expresses C-terminally EGFP-tagged IFT80 in mammalian cells

Depositor

Kazuhisa Nakayama

Insert

IFT80 (IFT80 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCAIP-hXRCC1wt-eGFP

Plasmid

#206032

Purpose

Mammalian expression of hXRCC1wt coupled to eGFP under the control of a CAG promoter

Depositor

Primo Schaer

Publication

Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 1

Insert

hXRCC1

Tags

eGFP

Expression

Mammalian

Promoter

CAG

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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PB-CAG-ChR2-YFP

Plasmid

#204345

Purpose

PiggyBac transposon vector suitable for inserting the ChR2-YFP optogenetic actuator driven by the ubiquitous CAG promoter into the genome of mammalian cell lines.

Depositor

Ivo Lieberam

Publication

Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi

Insert

ChR2-YFP

Tags

Yellow fluorescent protein

Expression

Mammalian

Mutation

C-terminus (aa315-737) is deleted - PMID: 1461559…

Promoter

CAG

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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BA31_pU.CAG.SaCas9-D10A.rBGpA

Plasmid

#199251

Purpose

Expression construct encoding the S. aureus Cas9.D10A nickase (SaCas9.D10A)

Depositor

Manuel Goncalves

Publication

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Insert

SaCas9.D10A nickase

Use

CRISPR

Tags

3XHA epitope, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

D10A

Promoter

CAG promoter

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-dDIO-MCS--4x6T

Plasmid

#196422

Purpose

Empty vector for astrocyte-selective Dre-dependent AAV expression; astrocyte selectivity generated with 4x6T miRNA targeting cassette

Depositor

Stanley Thomas Carmichael

Publication

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Type

Empty backbone

Use

AAV; Dre/rox; astrocyte-selective

Expression

Mammalian

Promoter

CAG

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-CAG-flex-lck-smV5

Plasmid

#196423

Purpose

Cre-dependent AAV expression of membrane-targeted V5 spaghetti monster reporter; no cell-type specificity

Depositor

Stanley Thomas Carmichael

Publication

Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

Insert

Lck-smV5

Use

AAV and Cre/Lox

Tags

Lck

Expression

Mammalian

Promoter

CAG

Available since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-CAG-hfYFP

Plasmid

#191201

Purpose

Constitutive CAG-driven expression of hfYFP in animals using AAV

Depositor

Gregory Petsko

Publication

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Hyperfolder YFP

Use

AAV

Expression

Mammalian

Mutation

mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

Promoter

CAG

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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UPB-duodenum (UPB6)

Plasmid

#180785

Purpose

Unique Projection Barcode (UPB6)-Duodenum for Projection-seq

Depositor

Rui Chang

Publication

Zhao et al Nature. 2022 Mar;603(7903):878-884. doi:

Insert

hM4D(Gi) (partial )

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-Kv-ArcLight-ST

Plasmid

#182319

Purpose

Soma-targeted fluorescent voltage indicator with left-shifted voltage-dependency

Depositor

Rafael Yuste

Publication

Bando et al Nat Commun. 2021 Dec 10;12(1):7229. doi:

Insert

Kv-ArcLight-ST

Expression

Mammalian

Mutation

Mutation in an amino acid Q217E of ArcLight-MT

Promoter

CAG

Available since

April 1, 2022

Availability

Academic Institutions and Nonprofits only

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pD2529-CAG-HA-mouse GARP

Plasmid

#180258

Purpose

Mouse full length GARP expression

Depositor

Timothy Springer

Publication

Jiang et al Cancer Immunol Res. 2022 Feb 18. pii: 68

Insert

GARP (Lrrc32 Mouse)

Tags

HA

Expression

Mammalian

Available since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-CIBN-EGFP-KRasCT

Plasmid

#178527

Purpose

Expresses CIBN-EGFP-KRasCT in mammalian cells

Depositor

Kazuhiro Aoki

Publication

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

CIBN-EGFP-KRasCT

Expression

Mammalian

Promoter

CAG

Available since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-CBA-DIO-GCaMP6s-P2A-mBeRFP-WPRE-pA

Plasmid

#175177

Purpose

Cre-dependent GCaMP6s and mBeRFP expression

Depositor

Bernardo Sabatini

Publication

Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

Insert

GCaMP6s

Use

AAV

Tags

P2A-mBeRFP

Promoter

CAG

Available since

Dec. 1, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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