We narrowed to 66,733 results for: %s
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Plasmid#145028PurposepET vector for IPTG-inducible, T7-based expression of the exonuclease deficient version of RTX bearing a C-terminal 6xHis tagDepositorPublicationInsertRTX(exo-)
Tags6xHisExpressionBacterialMutationN210DPromoterT7Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLV hUbC-dCas9 KRAB-T2A-GFP
Plasmid#67620PurposeCo-expressed human optimized S. pyogenese dCas9 fused to KRAB repressor domain and GFPDepositorInserthumanized dead Cas9 KRAB T2A GFP
UseCRISPR and LentiviralTagsFlagMutationD10A and H840AAvailable sinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSFFV-SpyTag-sfCherry2(11)-TagBFP
Plasmid#117485PurposeExpresses tandem tag (SpyTag002-GG-sfCherry2(11) fused to N-terminal of TagBFP) in mammalian cellsDepositorInsertSpyTag002-GG-sfCherry2(11)
UseLentiviralTagsTagBFPExpressionMammalianPromoterSFFVAvailable sinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pX459V2.0-eSpCas9(1.1)
Plasmid#108292PurposepX459 V2.0 (Plasmid #62988) with the K848A, K1003A, and R1060A mutationsDepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable sinceMay 21, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pINDUCER-21-RUNX1
Plasmid#97043PurposeExpresses RUNX1 in mammalian cellsDepositorInsertRunt Related Transcription Factor 1 (RUNX1 Human)
UseLentiviralExpressionMammalianPromoterTet onAvailable sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL4-ERSE2-luc2P-Hygro
Plasmid#101790PurposeLuciferase reporter plasmid containing three tandem repeats of the ER stress response element-II (ERSE-II)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert3xERSE-II
UseLuciferaseTagsLuciferaseExpressionMammalianPromoterminimal TATA-box promoter with low basal activityAvailable sinceSept. 27, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-Myc-GFP
Plasmid#83375PurposeExpression of Myc tagged GFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMyc-GFP
TagsMycExpressionMammalianPromoterCMVAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSH-Csy4-T2A-SpRFN-P2A-GFP-multi-gRNA
Plasmid#85756PurposeAll in one plasmid that expresses Csy4, S.pyogenes FokI-dCas9-NLS, and GFP. Also encodes for multiplexed gRNAs. Backbone derived from pSQT1601 (Addgene #53369).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCsy4-T2A-SpRFN-P2A-GFP
gRNA
UseCRISPRTagsCsy4, Csy4 recognition sequence, FokI fusion via …ExpressionMammalianMutationD10A, H840APromoterCMV and U6Available sinceJan. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCytERM_mScarlet-i_N1
Plasmid#85068PurposeIn vivo visualization of the ER (can be used for colocalization studies and the OSER assay)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCytERM
TagsmScarlet-iExpressionMammalianMutationaa1-29PromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJMP2
Plasmid#79874PurposeBacillus subtilis sgRNA expression vector; integrates into amyEDepositorInsertsgRNA RR1
UseCRISPRExpressionBacterialPromotervegAvailable sinceSept. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSMART-sgRNA (Sp)
Plasmid#80427PurposeU6 promoter driven sgRNA expression plasmid. Annealed oligonucleotides encoding crRNA sequence are ligated into BbsI site.DepositorTypeEmpty backboneExpressionMammalianAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hsyn-flex-dsRed-shvgat
Plasmid#67845PurposeExpresses shRNA to the vesicular GABA transporter VGAT. Cre dependent expression by flex switchDepositorInsertAAV-hsyn-flexed-dsRed-miR30-vgat2.shRNA
UseAAV, Cre/Lox, Mouse Targeting, and RNAiExpressionMammalianPromoterh-synapsinAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pYLsgRNA-OsU6b
Plasmid#66196Purposecloning of sgRNAs for expression in plantsDepositorTypeEmpty backboneUseCloning vectorPromoterOsU6bAvailable sinceAug. 10, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Str-KDEL_SBP-mCherry-Ecadherin
Plasmid#65287Purposesynchronize trafficking of Ecadherin from the ER (RUSH system)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEcadherin
TagsIL-2 Signal Sequence and Streptavidin Binding Pro…ExpressionMammalianPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAGGS-FuG-E
Plasmid#67509PurposeThis is an envelope gene encoding plasmid to make retrogradely lentiviral vector. If you use this plasmid, you can make type E envelope coating virus particle with NeuRet.DepositorInsertFusion Glycoprotein type E
UseLentiviralTagsFusion protein of Vesicular stomatitis Indiana vi…PromoterCAGGSAvailable sinceJuly 31, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHL-EF1a-SphcCas9-iP-A
Plasmid#60599PurposeExpresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCRISPR Cas9
UseCRISPRExpressionMammalianMutationCodon usage optimized for human usageAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mKate1.31-pBAD
Plasmid#54827PurposeLocalization: Protein Expression Vector , Excitation: 588, Emission: 635. Also known as mKate2.DepositorTypeEmpty backboneTags6xHis and mKate1.31 (aka mKate2)ExpressionBacterialAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKSI-1
Plasmid#51725Purposecloning backboneDepositorTypeEmpty backboneExpressionBacterialPromoterT7Available sinceJune 24, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKIKOlacZKm
Plasmid#46767PurposeE.coli genomic integration at lacZDepositorTypeEmpty backboneUseE.coli genomic integrationAvailable sinceSept. 24, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
drebrin-YFP
Plasmid#40359DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdrebrin E2 (DBN1 Human)
UseChick expressionTagseYFPPromoterchick B-actin promoter with CMV enhancerAvailable sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by