We narrowed to 10,110 results for: Ada

1. pAc5.1B-lambdaN-HA-DmGW182

   Plasmid

   #22406

   Depositor

   Elisa Izaurralde

   Article

   Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

   Insert

   gawky, variant A (gw Fly)
   

   Use

   Drosophila expression

   Tags

   lambdaN-HA

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. EC.SurA.TYB1.a172-274.wt

   Plasmid

   #15934

   Depositor

   David McKay

   Article

   Xu et al J Mol Biol. 2007 Oct 19. 373(2):367-81.

   Insert

   Peptidyl prolyl isomerase domain 1 of SurA (surA E. coli)
   

   Use

   Tags

   intein

   Expression

   Bacterial

   Mutation

   wild type

   Promoter

   Available Since

   Nov. 9, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUASp-Egl-Trbo

   Plasmid

   #179993

   Purpose

   Expresses Egl fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system.

   Depositor

   Graydon Gonsalvez

   Article

   Baker et al Development. 2021 Nov 15;148(22). pii: 2

   Insert

   Egl-Trbo
   

   Use

   Tags

   TurboID

   Expression

   Insect

   Mutation

   Promoter

   UAS-Gal4

   Available Since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAID1.2-CMV-NGFP-mAID

   Plasmid

   #140605

   Purpose

   Expression of OsTIR1 and GFP-mAID protein in DT40 cells under the control of CMV promoter

   Depositor

   Tatsuo Fukagawa

   Article

   Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

   Insert

   OsTIR1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEF-HA-Net1

   Plasmid

   #28264

   Depositor

   Jeffrey Frost

   Article

   Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

   Insert

   Net1 (Net1 Mouse)
   

   Use

   Tags

   2x HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 11, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pRS315e_pGal-dCas9-SH3

   Plasmid

   #79613

   Purpose

   Expresses dCas9-SH3 in yeast cells

   Depositor

   Akihiko Kondo

   Article

   Nishida et al Science. 2016 Aug 4. pii: aaf8729.

   Insert

   SpCas9
   

   Use

   Tags

   SH3 domain

   Expression

   Yeast

   Mutation

   D10A and H840A for nuclease deficient Cas9

   Promoter

   pGal1

   Available Since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. sspa-GCaMP6s

   Plasmid

   #67559

   Purpose

   short photoactivatable fluorescent calcium reporter

   Depositor

   Ehud Isacoff

   Article

   Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

   Insert

   spa-GCaMP6s
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

   Promoter

   Available Since

   July 28, 2015

   Availability

   Academic Institutions and Nonprofits only

8. EK0499 SFFV-mCherry-SGc(PPIB.s1)cSG-EGFP-(3xMS2) (FLP-IN)

   Plasmid

   #191183

   Purpose

   Expression of PPIB sensor in mammalian cells

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:PPIB.s1:EGFP-3xMS2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. EK0289 SFFV-mCherry-SG(s17d9b)SG-EGFP (FLP-IN)

   Plasmid

   #191142

   Purpose

   Expression of Sensor 2 (for EK0211) in mammalian cells; mCherry marker, EGFP output.

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:s17d9b:EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   SFFV

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGEX-KG/Net1

    Plasmid

    #28260

    Depositor

    Jeffrey Frost

    Article

    Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

    Insert

    Net1 (Net1 Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 11, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pAID1.2-CMV-NmScarlet-mAID

    Plasmid

    #140618

    Purpose

    Expression of OsTIR1 and mScarlet-mAID protein in DT40 cells under the control of CMV promoter

    Depositor

    Tatsuo Fukagawa

    Article

    Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

    Insert

    OsTIR1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-SLAP-FLAG

    Plasmid

    #74500

    Purpose

    Overexpression of SLAP in mammalian cells

    Depositor

    Lars Rönnstrand

    Article

    Kazi et al PLoS One. 2012;7(12):e53509. doi: 10.137

    Insert

    SLAP (SLA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N18K, G74A, and T149A

    Promoter

    CMV

    Available Since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pRSET-triple mutation-pa-GCaMP6f

    Plasmid

    #67570

    Purpose

    bacterial expression of a photoactivatable fluorescent calcium reporter

    Depositor

    Ehud Isacoff

    Article

    Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

    Insert

    pa-GCaMP6f
    

    Use

    Tags

    HIS-tag

    Expression

    Bacterial

    Mutation

    V115H, L64F, T65S

    Promoter

    T7

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pCFJ1360 - Intron GG3 - smu-1

    Plasmid

    #159806

    Purpose

    Golden Gate compatible intron with PATCs for reduced germline silencing

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    Intron GG3 - smu-1
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCFJ1358 - Intron GG1 - smu-1

    Plasmid

    #159804

    Purpose

    Golden Gate compatible intron with PATCs for reduced germline silencing

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    Intron GG1 - smu-1
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pZA13

    Plasmid

    #158491

    Purpose

    Entry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 without STOP codon

    Depositor

    Sophien Kamoun

    Article

    Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

    Insert

    ZAR1
    

    Use

    Golden gate entry vector

    Tags

    Expression

    Mutation

    no STOP codon

    Promoter

    Available Since

    Sept. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. px330 EQR

    Plasmid

    #101733

    Purpose

    Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 EQR
    

    Use

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

    Promoter

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCFJ2214 - Intron GG1 - 900 bp

    Plasmid

    #159880

    Purpose

    Golden Gate compatible intron with PATCs for reduced germline silencing

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    Intron GG1 - 900 bp
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTW324

    Plasmid

    #115955

    Purpose

    Destination Vector

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl1 P1 End
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTW064

    Plasmid

    #115951

    Purpose

    Destination Vector

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl0 ORF Dest
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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