We narrowed to 7,757 results for: crispr c plasmids

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LLP791_PB_dCas9-SSSavi_BirA

Plasmid

#211786

Purpose

PiggyBac plasmid with dCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), and BirA, with Hygro selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

dCas9, Spy, Snoop, aGCN4, AviTags

Tags

3xHA

Expression

Mammalian

Mutation

dCas9 D10A and H840A

Promoter

pCAGG abd pEF1a

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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OA-16

Plasmid

#104966

Purpose

Plasmid expressing two multiplexed ribozyme-gRNA-ribozyme (RGR) gRNAs

Depositor

Omar Akbari

Article

Marshall et al Sci Rep. 2017 Jun 19;7(1):3776. doi: 10.

Insert

Ribozyme-gRNA-ribozyme

Use

Cre/Lox

Expression

Insect

Available Since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-cTNT-Cre

Plasmid

#209785

Purpose

Expresses Cre by the specific cTNT promoter

Depositor

Yuxuan Guo

Article

Yang et al Circulation. 2024 May 28;149(22):1778-17

Insert

Cre

Use

AAV

Promoter

cTNT

Available Since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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Sha2Cas9

Plasmid

#192140

Purpose

Expresses Sha2Cas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

Insert

Sha2Cas9

Use

AAV

Expression

Mammalian

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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SpeCas9-HF

Plasmid

#192141

Purpose

Expresses highly specific SpeCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al PLoS Biol. 2022 Nov 30;20(11):e3001897.

Insert

SpeCas9-HF

Use

AAV

Expression

Mammalian

Mutation

SpeCas9-HF (R247A, N415A, S421A)

Available Since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pJEP306-pAAV-EFS-MCS3-pA

Plasmid

#113682

Purpose

EFS driven Multi Cloning Site-3.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Insert

N/A

Use

AAV

Promoter

Cytomegalo Virus(CMV)

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA16_Lmo-NLS(SV40) (KAC508)

Plasmid

#134332

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA16_Lmo with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA16_Lmo

Tags

NLS(SV40)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA17_Sga-NLS(SV40) (KAC92)

Plasmid

#134334

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA17_Sga with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA17_Sga

Tags

NLS(SV40)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA18_Sma-NLS(SV40) (KAC95)

Plasmid

#134335

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA18_Sma with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA18_Sma

Tags

NLS(SV40)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA19_Ssim-NLS(SV40) (KAC97)

Plasmid

#134336

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA19_Ssim with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA19_Ssim

Tags

NLS(SV40)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA18_Sga-NLS(SV40) (KAC214)

Plasmid

#134338

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA18_Sga with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA18_Sga

Tags

NLS(SV40)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-T7-AcrIIA19_Spse-NLS(SV40) (KAC215)

Plasmid

#134339

Purpose

CMV and T7 promoter expression plasmid for human codon optimized AcrIIA19_Spse with C-terminal NLS (SV40)

Depositor

Joseph Bondy-Denomy, Benjamin Kleinstiver

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

human codon optimized AcrIIA19_Spse

Tags

NLS(SV40)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pHelp

Plasmid

#227583

Purpose

Accessory plasmid with a sgRNA cassette for Cas9, and ampicillin resistance

Depositor

Chengzhang Fu

Article

Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

Insert

guide RNA scaffold

Use

Cloning vector

Available Since

Dec. 17, 2024

Availability

Academic Institutions and Nonprofits only

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ABEmax-Puro V2

Plasmid

#226955

Purpose

ABEmax plasmid enabling A:T to G:C base editing using a single plasmid.

Depositor

Paul Thomas

Article

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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BE3-hA3A-Y130F

Plasmid

#132945

Purpose

Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.

Depositor

Hui Yang

Article

Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

Insert

BE3-hA3A(Y130F)

Use

CRISPR; Base editor

Expression

Mammalian

Mutation

BE3-hA3A(Y130F)

Available Since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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LLP789_αGCN4-DNMT3A-CO

Plasmid

#211783

Purpose

SunTag counterpart binding domain, aGCN4, fused to DNA methyltransferase DNMT3A, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

aGCN4-DNMT3A

Tags

3xTy1

Expression

Mammalian

Mutation

Last 300 bp codon optimised (CO) to detect DNMT3A…

Promoter

pEF1a and pSV40

Available Since

Dec. 13, 2023

Availability

Academic Institutions and Nonprofits only

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1781_pAAV-U6-Ai9-Sa-gRNA1-CB-SACas9-HA-OLLAS-spA_C

Plasmid

#135979

Purpose

gRNA against the left loxP site of the Ai9 allele

Depositor

William Lagor

Insert

Ai9 gRNA1

Use

AAV, CRISPR, and Mouse Targeting

Tags

HA

Promoter

U6

Available Since

Jan. 23, 2020

Availability

Academic Institutions and Nonprofits only

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qTAG-C-Solo-mScarlet-MAPRE1

Plasmid

#227323

Purpose

Donor template for mScarlet insertion into the C-terminus of the MAPRE1 locus. For growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 (Addgene #207793)

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

MAPRE1 Homology Arms flanking a mScarlet Tag (MAPRE1 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Promoter

Promoterless

Available Since

Nov. 25, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mStayGold-EFS-Puro-PLK4

Plasmid

#227311

Purpose

Donor template for mStayGold-EFS-Puro insertion into the C-terminus of the PLK4 locus. For centriole visualization. To be co-transfected with sgRNA plasmid px458-PLK4 (Addgene #227310)

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

PLK4 Homology Arms flanking a mStayGold-EFS-Puro Cassette (PLK4 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Promoter

Promoterless

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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qTAG-C-Solo-mStayGold-CEP192

Plasmid

#227289

Purpose

Donor template for mStayGold insertion into the C-terminus of the CEP192 locus. For pericentriolar material visualization. To be co-transfected with sgRNA plasmid px330-PITCh-CEP192 (Addgene #227288)

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

CEP192 Homology Arms flanking a mStayGold Tag (CEP192 Human)

Use

CRISPR; Donor template

Expression

Mammalian

Promoter

Promoterless

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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