We narrowed to 199,288 results for: ins

1. pmCherry-C1 actin-3XNLS P2A mCherry

   Plasmid

   #58475

   Purpose

   Expresses nuclear-targeted human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

   Inserts

   beta actin (ACTB Human)
   3XNLS
   P2A cleavage site
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. G protein alpha-q-GFP

   Plasmid

   #66080

   Purpose

   This G protein alpha-q construct contains internal insertions of GFP and the EE epitope

   Depositor

   Catherine Berlot

   Article

   Hughes et al J Biol Chem. 2001 Feb 9;276(6):4227-35.

   Insert

   G protein alpha-q-EE-YFP (Gnaq Mouse)
   

   Tags

   GFP was inserted internally into the alpha-q sequ…

   Expression

   Mammalian

   Mutation

   BamHI and SacI sites in alpha-q were removed with…

   Promoter

   CMV

   Available Since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pmCherry-C1 R62D actin-3XNLS P2A mCherry

   Plasmid

   #58477

   Purpose

   Expresses nuclear-targeted non-polymerizing R62D mutant of human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

   Depositor

   Dyche Mullins

   Article

   Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

   Inserts

   beta actin (ACTB Human)
   3XNLS
   P2A cleavage site
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Changed Arginine 62 to Aspartic Acid

   Promoter

   CMV

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLX_TRC311 ETV5

   Plasmid

   #74984

   Purpose

   Constitutive ETV5 ORF (codon optimized), blasticidin selection

   Depositor

   William Hahn

   Article

   Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

   Inserts

   ETV5 (ETV5 Human)
   ETV5 (ETV5 Human)
   

   Expression

   Mammalian

   Mutation

   Codon optimized (synonymous)

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ikaros-CRISPR-Nick2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

   Plasmid

   #75243

   Purpose

   CRISPR/Cas9 NICKASE plasmid against human Ikaros (2/2)

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against human Ikaros (IKZF1 Human)
   

   Use

   CRISPR

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. MK01_HUMAN_D0

   Plasmid

   #79713

   Purpose

   This plasmid encodes the kinase domain of MK01. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

   Depositor

   John Chodera, Nicholas Levinson, Markus Seeliger

   Article

   Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

   Insert

   MK01 (MAPK1 Human)
   

   Tags

   His10-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

7. Ikaros-CRISPR-Nick 1/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

   Plasmid

   #75242

   Purpose

   CRISPR/Cas9 NICKASE plasmid against human Ikaros (1/2)

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   sgRNA against human Ikaros (IKZF1 Human)
   

   Use

   CRISPR

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. Rb379-928/C706F-pGex2TK

   Plasmid

   #21750

   Depositor

   William Kaelin

   Article

   Kaelin et al Cell. 1992 Jul 24. 70(2):351-64.

   Insert

   Rb (RB1 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   deletion mutant ORF contains aa 379-928 of wt Rb …

   Available Since

   Sept. 30, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1 Flag YFP hNKCC1 P676C I730C N-terminal deletion C-terminus replaced NBCe C-terminus (NT94)

   Plasmid

   #50912

   Purpose

   Expresses human NKCC1 with truncation of N-terminus, mutations at P676C I730C and C-terminus replaced with that of NBCe. Contains an N-terminal 3xFLAG-YFP tag for expression in mammalian cells

   Depositor

   Biff Forbush

   Article

   Monette et al J Biol Chem. 2014 Jan 22.

   Insert

   human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
   

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   P676C and I730C; N-term deletion of aa13-222; C-t…

   Promoter

   CMV

   Available Since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3.1 Flag YFP hNKCC1 N-terminus T,S mutations P676C I730C (NT95)

    Plasmid

    #50913

    Purpose

    Expresses human NKCC1 with mutated Ser and Thr residues in the N-terminus and mutations at P676C I730C. Contains N-terminal 3xFLAG-YFP tag for expression in mammalian cells

    Depositor

    Biff Forbush

    Article

    Monette et al J Biol Chem. 2014 Jan 22.

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    S150N S155Q S170Q T177A S183R S193E T203A T205A T…

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFastBac LIC cloning vector (4A)

    Plasmid

    #30111

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. mRuby-BaxAlpha-C-15

    Plasmid

    #55847

    Purpose

    Localization: , Excitation: 558, Emission: 605

    Depositor

    Michael Davidson

    Insert

    BaxAlpha
    

    Tags

    mRuby

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 12, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

13. EGFP-BaxAlpha-C-15

    Plasmid

    #56360

    Purpose

    Localization: , Excitation: 488, Emission: 507

    Depositor

    Michael Davidson

    Insert

    BaxAlpha
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 6, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

14. EYFP-Cx43-7

    Plasmid

    #56587

    Depositor

    Michael Davidson

    Insert

    Cx43
    

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. EYFP-Cx26-7

    Plasmid

    #56585

    Depositor

    Michael Davidson

    Insert

    Cx26
    

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 23, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

16. mTurquoise-Rab5a-7

    Plasmid

    #55578

    Purpose

    Localization: Ras GTPase / Endosomes, Excitation: 434, Emission: 474

    Depositor

    Michael Davidson

    Insert

    Rab5a
    

    Tags

    mTurquoise

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

17. mTagGFP2-Rab5a-7

    Plasmid

    #56417

    Purpose

    Localization: Ras GTPase, Excitation: 483, Emission: 506

    Depositor

    Michael Davidson

    Insert

    Rab5a
    

    Tags

    mTagGFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 28, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pFastBac His6 TEV LIC cloning vector (4B)

    Plasmid

    #30115

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6-TEV

    Expression

    Insect

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFastBac Dual LIC cloning vector (5A)

    Plasmid

    #30121

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Expression

    Insect

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mTagBFP-Rab5a-7

    Plasmid

    #55267

    Purpose

    Localization: Ras GTPase, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Insert

    Rab5a
    

    Tags

    mTagBFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits