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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 18,344 results for: multi

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Showing: 3281 - 3300 of 18344 results

  1. Gβ-2A-cpV-Gγ2-IRES-Gαi1-mTq2

    Plasmid
    #69623
    Purpose
    A FRET sensor for Galphai1 activation, encoded on a single plasmid.
    Depositor
    Dorus Gadella
    Publication
    van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Gbeta-2A-cpV-Ggamma2
    Galphai-mTurquoise2
    Tags
    ciruclar permutated Venus (cp173V) and mTurquoise…
    Expression
    Mammalian
    Promoter
    CMV and CMV (IRES)
    Available since
    Jan. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    69623-plasmid-map-sequence-id-459280

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  2. AAV:ITR-U6-sgRNA(NeuN)-hSyn-Cre-2A-EGFP-KASH-WPRE-shortPA-ITR

    Plasmid
    #60230
    Purpose
    Expresses Cre recombinase and KASH-tagged EGFP from the hSyn promoter and one U6-driven sgRNA. AAV backbone with SapI spacer for sgRNA cloning.
    Depositor
    Feng Zhang
    Publication
    Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01
    Inserts
    sgRNA
    Cre recombinase
    EGFP
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Cre-HA-P2A and EGFP-KASH
    Expression
    Mammalian
    Promoter
    U6 and hSyn
    Available since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    60230-plasmid-map-sequence-id-490567

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  4. pAC95-pmax-dCas9VP160-2A-neo

    Plasmid
    #48227
    Purpose
    dCas9VP160-2A-neo (neo/G418-selectable) on pmax expression vector. Note: This is being tested.
    Depositor
    Rudolf Jaenisch
    Publication
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Insert
    dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-neo
    Use
    CRISPR
    Tags
    2A-neo, HA Tag, and VP160
    Expression
    Mammalian
    Mutation
    D10A;H840A (catalytically inactive)
    Promoter
    CAGGS
    Available since
    Sept. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    48227-plasmid-map-sequence-id-69517

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  6. pGolt-mCherry / pGolt3-mCherry

    Plasmid
    #73297
    Purpose
    Visualization of Dendritic Golgi Satellite
    Depositor
    Michael Kreutz
    Publication
    Mikhaylova et al Cell Rep. 2015 Dec 30. pii: S2211-1247(1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    ER export signal of Scap
    ER export signal of Scap
    TMD (trans-membrane domain) of Calneuron-2 (Cabp7 Rat)
    Tags
    ER export and mCherry
    Expression
    Mammalian
    Promoter
    CMV immediate early promoter
    Available since
    Feb. 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    73297-plasmid-map-sequence-id-135341

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  12. lentiCRISPR - EGFP sgRNA 3

    Plasmid
    #51762
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Publication
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 3
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    51762-plasmid-map-sequence-id-79380

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Showing: 3281 - 3300 of 18344 results