169,099 results

1. mEGFP-NbALFA

   Plasmid

   #159986

   Purpose

   For the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Insert

   mEGFP-NbALFA
   

   Tags

   8histidine-HRV3C protease cleavage site

   Expression

   Bacterial

   Mutation

   3GS linker between mEGFP and NbALFA

   Available Since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 Ash2L full

   Plasmid

   #15548

   Depositor

   Kai Ge

   Article

   Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

   Insert

   Ash2L (ASH2L Human)
   

   Expression

   Mammalian

   Mutation

   R622H (Please see depositor comments below)

   Available Since

   Aug. 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pZac2.1 GfaABC1D hM3D mCherry SV40

   Plasmid

   #92284

   Purpose

   AAV DREADD expression

   Depositor

   Baljit Khakh

   Article

   Chai et al Neuron. 2017 Aug 2;95(3):531-549.e9. doi

   Insert

   hM3D (CHRM3 Human)
   

   Use

   AAV

   Tags

   mCherry

   Promoter

   GfaABC1D

   Available Since

   Aug. 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. EGFP-E-Syt2

   Plasmid

   #66831

   Purpose

   Mammalian Expression, Bacterial Expression

   Depositor

   Pietro De Camilli

   Article

   Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

   Insert

   E-Syt2 (ESYT2 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CaMKII-PinkyCaMP

   Plasmid

   #232858

   Purpose

   An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under CaMKII promoter

   Depositor

   Olivia Masseck

   Article

   Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

   Insert

   PinkyCaMP
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CaMKII

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLOVE-miR302/367

   Plasmid

   #31310

   Depositor

   Ed Morrisey

   Article

   Anokye-Danso et al Cell Stem Cell. 2011 Apr 8. 8(4):376-88.

   Insert

   mmu-miR302/367
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Oct. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

7. TRUPATH Triple Gaq

   Plasmid

   #196058

   Purpose

   Encodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphaQ-RLuc8 (GNAQ Human)
   GGamma9-GFP2
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available Since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.Syn.NES-jRGECO1a.WPRE.SV40

   Plasmid

   #100854

   Purpose

   AAV expression of jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

   Depositor

   Douglas Kim, GENIE Project

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, and AAV9

   Insert

   jRGECO1a
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. ABE-NW1

   Plasmid

   #240615

   Purpose

   Adenine base editor with narrowed editing window

   Depositor

   Tingting Jiang

   Article

   Valdez et al Nat Commun. 2025 Aug 30;16(1):8115. doi:

   Insert

   TadA8e(N108Q,M151C,Q154Y)-linker-nCas9 (D10A)
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   TadA8e(N108Q,M151C,Q154Y),Cas9 (D10A)

   Available Since

   July 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCas12f1

    Plasmid

    #171182

    Purpose

    Constitutive expression of cas12f1, arabinose-inducible expression of lambda recombinase, and IPTG-inducible expression of sgRNA for curing of pTarget12f1

    Depositor

    Kohsuke Honda

    Article

    Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

    Insert

    Cas12f1
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    cas9 promoter from Streptococcus pyogenes

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-Cas9-gRNA-GFP

    Plasmid

    #124770

    Purpose

    3rd generation lentiviral plasmid encoding Cas9, GFP, and a gRNA backbone

    Depositor

    Jason Sheltzer

    Article

    Giuliano et al Curr Protoc Mol Biol. 2019 Sep;128(1):e1

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. AiP13779 - pAAV-AiE0779m_3xC2-minBG-iCre(R297T)-BGHpA (Alias: CN3779)

    Plasmid

    #199776

    Purpose

    enhancer AAV for Cre recombinase expression in striatal direct pathway D1-MSNs

    Depositor

    Allen Institute for Brain Science, Jonathan Ting

    Article

    Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

    Has Service

    AAV PHP.eB

    Insert

    iCre(R297T)
    

    Use

    AAV

    Mutation

    R297T for reduced recombinase efficiency.

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYESir2-puro

    Plasmid

    #1769

    Depositor

    Bob Weinberg

    Article

    Vaziri et al Cell 2001 Oct 19;107(2):149-59.

    Insert

    Sir2 (SIRT1 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    June 3, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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14. PTGS1-bio-His

    Plasmid

    #53414

    Purpose

    Expresses full-length Prostaglandin G/H synthase 1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    PTGS1 (PTGS1 Human)
    

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJSK663

    Plasmid

    #205129

    Purpose

    Expression of GFP-1xPX (from SnxA) in mammalian cells

    Depositor

    Jason King

    Article

    Vines et al J Cell Biol. 2023 Sep 4;222(9):e20220907

    Insert

    1xPX domain of SnxA
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Sept. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV.cTNT.iCre

    Plasmid

    #69916

    Purpose

    Used to package AAV9:cTNT.iCre, which specifically transduce cardiomyocytes and express Cre recombinase. Only works in vivo.

    Depositor

    William Pu

    Article

    Lin et al Circ Res. 2015 Jan 2;116(1):35-45. doi:

    Has Service

    AAV9

    Insert

    improved cre (iCre) recombinase and tdTomato
    

    Use

    AAV

    Tags

    The iCre coding frame and tdTomato coding frame i…

    Promoter

    Chicken cardiac troponin T

    Available Since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWZL-Blast FAPwt

    Plasmid

    #210643

    Purpose

    Expresses the FAP (Fibroblast Activation Protein) protein.

    Depositor

    Jing Yang

    Article

    Bukhari et al Cell Rep. 2023 Oct 31;42(10):113302. doi

    Insert

    Fibroblast Activation Protein Alpha (FAP Human)
    

    Use

    Retroviral

    Mutation

    none

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCCL-dNTP63Promoter-FLuc-CMV-RLuc-dsRed2

    Plasmid

    #215326

    Purpose

    Lentiviral mammalian vector with firefly luciferase reporter for dNTP63 expression; renilla luciferase and dsRed2 reporter under CMV promoter.

    Depositor

    Rob Hynds

    Article

    Orr et al Am J Physiol Lung Cell Mol Physiol. 2024

    Insert

    dNTP63 Promoter
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    dNTP63

    Available Since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTarget12f1

    Plasmid

    #171183

    Purpose

    Constitutive expression of sgRNA without donor DNA

    Depositor

    Kohsuke Honda

    Article

    Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

    Insert

    sgRNA scaffold in the CRISPR/Cas12f1 system
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    J23119(SpeI) promoter

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-Ef1a-DIO SwiChRca-TS-EYFP

    Plasmid

    #55631

    Purpose

    Cre-Dependent Chloride Channel

    Depositor

    Karl Deisseroth

    Article

    Berndt et al Science. 2014 Apr 25;344(6182):420-4. do

    Insert

    iC1C2-TS-EYFP
    

    Use

    AAV and Cre/Lox

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    C128A

    Promoter

    Ef1a

    Available Since

    Aug. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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