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pDONR223-MCU-S259A

Plasmid

#31728

Depositor

Vamsi Mootha

Publication

Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

Insert

MCU (MCU Human)

Use

Pdonr gateway

Tags

None

Mutation

Changed S259 to Alanine

Available since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
pDONR223-MCU-E257A

Plasmid

#31727

Depositor

Vamsi Mootha

Publication

Baughman et al Nature. 2011 Jun 19;476(7360):341-5. doi

Insert

MCU (MCU Human)

Use

Pdonr gateway

Tags

None

Mutation

Changed E257 to Alanine

Available since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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HA-VHL Y98H-pRc/CMV

Plasmid

#22044

Depositor

William Kaelin

Publication

Ohh et al Mol Cell. 1998 Jun . 1(7):959-68.

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Tags

HA-tag

Expression

Mammalian

Mutation

point mutant, converting aa 98 from Tyrosine to H…

Available since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pCDB179-TurboPETase

Plasmid

#218693

Purpose

Encodes codon optimized TurboPETase enzyme for bacterial expression

Depositor

Gregg Beckham

Publication

Norton-Baker et al Sci Rep. 2024 Jun 24;14(1):14449. doi: 1

Insert

TurboPETase

Tags

10xHis

Expression

Bacterial

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mBlueberry2-P2A-EGFP

Plasmid

#184937

Purpose

Expresses blue fluorescent protein mBlueberry2 and EGFP in mammalian cells

Depositor

Kiryl Piatkevich

Publication

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

mBlueberry2-P2A-EGFP

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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AAV-Ef1a-CreN-InteinN

Plasmid

#187614

Purpose

AAV vector that encodes N-terminal portion of split-Intein-mediated split-Cre

Depositor

Il Hwan Kim

Publication

Kim et al Cell Rep. 2022 Jun 7;39(10):110906. doi:

Insert

CreN-InteinN

Use

AAV

Expression

Mammalian

Available since

July 21, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONRPEX18Tp-SceI-pheS

Plasmid

#111608

Purpose

Gateway compatible allelic exchange vector for Burkholderia sp.

Depositor

Tim Tolker-Nielsen

Publication

Fazli et al Appl Environ Microbiol. 2015 Jun;81(11):

Type

Empty backbone

Use

Allelic exchange in burkholderia sp.

Available since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEYFP-N1-eKR2

Plasmid

#115337

Purpose

Expresses enhanced sodium pump for light-driven extrusion of sodium; activation with green light; codon-optimized for human/mouse

Depositor

Peter Hegemann

Publication

Grimm et al Sci Rep. 2018 Jun 18;8(1):9316. doi: 10.

Insert

eKR2

Tags

EYFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTRIP-SFFV-mtagBFP-2A STINGHAQ

Plasmid

#102587

Purpose

Lentivector to express STING HAQ and mTagBFP

Depositor

Nicolas Manel

Publication

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

R71H-G230A-R293Q (HAQ)

Promoter

SFFV

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUB-nHyPer2 (NLS:HyPer2:NLS)

Plasmid

#84730

Purpose

Expresses HyPer2 in plant cells-biosensor for Hydrogen peroxide targeted to Nuclei

Depositor

Philip Mullineaux

Publication

Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10

Insert

HyPer2 targeted to nucleus

Tags

NLS (nuclear localisation signal and NLS (nuclear…

Expression

Plant

Promoter

ubiqutin- 10 gene promoter (pUBQ10) of Arabidopsis

Available since

July 6, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_Flag_PHC3

Plasmid

#45262

Depositor

William Hahn

Publication

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

PHC3 (PHC3 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available since

May 23, 2013

Availability

Academic Institutions and Nonprofits only
pCMV10-3xFLAG-prs-XK

Plasmid

#184369

Purpose

Express human XK in Expi293 cells

Depositor

Karin Reinisch

Publication

Adlakha et al J Cell Biol. 2022 Jun 6;221(5):e20220203

Insert

Human XK (XK Human)

Tags

3xFLAG-PreScission cleavage site

Expression

Mammalian

Promoter

CMV

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pJ2018

Plasmid

#140665

Purpose

dCas9, LuxR & Csy4

Depositor

Yolanda Schaerli

Publication

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

dCas9, LuxR & Csy4

Use

Synthetic Biology

Expression

Bacterial

Available since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pJ2048.2

Plasmid

#140687

Purpose

3-color stripe (different sgRNAs)

Depositor

Yolanda Schaerli

Publication

Santos-Moreno et al Nat Commun. 2020 Jun 2;11(1):2746. doi:

Insert

3-color stripe (different sgRNAs)

Use

Synthetic Biology

Expression

Bacterial

Available since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAC1815_pCR8-gSMN2-DN3

Plasmid

#118648

Purpose

Transient transfection; Expressed SMN2-DN3 CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Publication

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Insert

gSMN2-DN3

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCD315

Plasmid

#113321

Purpose

pBAD-W108 scRNA.b1

Depositor

Jesse Zalatan

Publication

Dong et al Nat Commun. 2018 Jun 27;9(1):2489. doi:

Insert

scRNA.b1_MS2

Use

CRISPR

Expression

Bacterial

Available since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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pRB35

Plasmid

#72866

Purpose

template base vector for N-terminal tagging, constitutive Puromycin resistance

Depositor

Klaus Foerstemann

Publication

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

base vector for N-terminal tags, Puro

Expression

Insect

Available since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-PM-Cpalm-HA-SrtA (pKS37)

Plasmid

#50031

Purpose

Expression of S. pyogenes SrtA at the plasma membrane in mammalian cells

Depositor

Hidde Ploegh

Publication

Strijbis et al Traffic. 2012 Jun;13(6):780-9. doi: 10.1

Insert

PM-SrtA(strep)

Tags

C-HA

Expression

Mammalian

Promoter

pCMV

Available since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pASK/3xMBT

Plasmid

#28234

Depositor

Danny Reinberg

Publication

Trojer et al Cell. 2007 Jun 1. 129(5):915-28.

Insert

l(3)mbt-like 1 (Drosophila) (L3MBTL1 Human)

Tags

His

Expression

Bacterial

Mutation

Truncation expressing only aa 252-606 (MBT repeat…

Available since

May 23, 2011

Availability

Academic Institutions and Nonprofits only
pMT-Mad2-GFP

Plasmid

#24284

Depositor

Ron Vale

Publication

Griffis et al J Cell Biol. 2007 Jun 18. 177(6):1005-15

Insert

Mad2 (mad2 Fly)

Tags

GFP

Expression

Insect

Available since

May 10, 2010

Availability

Academic Institutions and Nonprofits only