We narrowed to 7,211 results for: CIT

1. Venus-Noxa-4E-pEGFP-C1

   Plasmid

   #166745

   Purpose

   Express Venus fused to the N-terminus of the Bcl-2 family protein, Noxa with mutation in BH3 domain to disrupt binding anti-apoptotic proteins

   Depositor

   David Andrews

   Article

   Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

   Insert

   Noxa-4E (PMAIP1 Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   4 hydrophobic residues in the BH3 region mutated …

   Promoter

   CMV

   Available since

   April 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-FLEX-rc[Chronos-GFP]

   Plasmid

   #59056

   Purpose

   Expression vector for Chronos-GFP in a floxed/reversed, Cre-dependent manner (Cre turns gene on)

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   Chronos-GFP
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Aug. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. MBP-FUS_LC_12E

   Plasmid

   #98654

   Purpose

   Expresses MBP-tagged phosphomimetic FUS AA 1-163

   Depositor

   Nicolas Fawzi

   Article

   Monahan et al EMBO J. 2017 Aug 8. pii: e201696394. doi

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   6xHis-MBP

   Expression

   Bacterial

   Mutation

   12 S/T to E

   Promoter

   Available since

   Aug. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pD2sE_SC10

   Plasmid

   #219716

   Purpose

   DV2 stable E dimer with fusion loop mutation

   Depositor

   Brian Kuhlman

   Article

   Kudlacek et al Sci Adv. 2021 Oct 15;7(42):eabg4084. doi

   Insert

   DV2 sE SC10
   

   Use

   Tags

   8xHis and human serum albumin (HSA)

   Expression

   Mammalian

   Mutation

   G106D, A259W, T262R, F279W, T280P

   Promoter

   Available since

   Aug. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. 426Gal-FUS

   Plasmid

   #29591

   Depositor

   Aaron Gitler

   Article

   Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

   Insert

   FUS (FUS Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   July 1, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pDestTol2-actinb-kcnk9-IRES-EGFP

   Plasmid

   #164949

   Purpose

   Tol2 construct: actinb (actb2) promoter driven zebrafish kcnk9 and EGFP

   Depositor

   GuangJun Zhang

   Article

   Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

   Insert

   kcnk9 (kcnk9 Zebrafish)
   

   Use

   Tol2 transposon destination vector

   Tags

   IRES-EGFP

   Expression

   Mutation

   Promoter

   actinb (actb2) promoter

   Available since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLEX307-HCoV-NL63-3CL

   Plasmid

   #160286

   Purpose

   Allows for constituitive mammalian expression of the HCoV-NL63 3CL protease via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV hSyn FLEx-OFF Kir2.1-2A-GFP

   Plasmid

   #161580

   Purpose

   Express Kir2.1 in cells not expressing Cre.

   Depositor

   Kevin Beier, Robert Malenka

   Article

   Beier et al Nature. 2017 Sep 21;549(7672):345-350. d

   Insert

   Kir2.1-2A-GFP (Kcnj2 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLEX307-Bat-CoV-HKU9-3CL

   Plasmid

   #160284

   Purpose

   Allows for constituitive mammalian expression of the Bat-CoV-HKU9 3CL protease via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEF073 MBP-Axin-His

    Plasmid

    #154011

    Purpose

    Expresses MBP-Axin-His (human Axin as a fusion protein with MBP and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    Axin1 (AXIN1 Human)
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. 416Gal-FUS-R521C-YFP

    Plasmid

    #29622

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    R521C

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pLEX307-SARS-CoV-3CL

    Plasmid

    #160280

    Purpose

    Allows for constituitive mammalian expression of the SARS-CoV 3CL protease via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. xCas12i

    Plasmid

    #195333

    Purpose

    vector for encoding a human codon-optimized xCas12i driven by CBh promoter, guide RNAs compatible with xCas12i driven by hU6, and mCherry driven by CMV promoter

    Depositor

    Yingsi Zhou

    Article

    Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

    Insert

    human codon-optimized xCas12i
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CBh, CMV, hU6

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLC-CUL4A K705R-P2A-Puro

    Plasmid

    #124305

    Purpose

    Lentiviral vector for expression of Nedd8 attachment mutant K705R CUL4A-P2A-Puro casette from a CMV promoter

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    CUL4A (CUL4A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    changed Lysine 705 to Arginine

    Promoter

    CMV

    Available since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGAL4-DBD-CEBPa-WT

    Plasmid

    #215601

    Purpose

    For overexpression of Gal4-DBD CEBPa WT

    Depositor

    Denes Hnisz

    Article

    Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

    Insert

    CEBPa (CEBPA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. FCK-Chronos-GFP

    Plasmid

    #80902

    Purpose

    Lenti-mediated expression of Chronos-GFP under the CaMKII promoter.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Insert

    Chronos-GFP
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII

    Available since

    March 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUPD2 sgRNAscaffold 2.1 scRNA (GB1437)

    Plasmid

    #160571

    Purpose

    Version of SgRNA scaffold with the sequence 2.1 of Ms2 aptamer in the 3' end.

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    sgRNAscaffold 2.1 scRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA 3.2-FUS-1-413aa-V5

    Plasmid

    #29612

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS-1-413aa (FUS Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Contains FUS aa# 1-413

    Promoter

    Available since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

19. 416Gal-FUS-R521G-YFP

    Plasmid

    #29623

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    R521G

    Promoter

    Available since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

20. 3I91

    Plasmid

    #62514

    Purpose

    Bacterial expression plasmid for use in structural determination.

    Depositor

    Cheryl Arrowsmith

    Article

    Kaustov et al J Biol Chem. 2011 Jan 7;286(1):521-9. do

    Insert

    CBX8 (CBX8 Human)
    

    Use

    Tags

    6xHis and TEV cleavage

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits