We narrowed to 11,272 results for: HAL

1. pcDNA5FRTTO-NHASt-codoptUL50

   Plasmid

   #135101

   Purpose

   Codon-optimized HSV-1 UL50 with N-terminal HAStrep tag

   Depositor

   Markus Landthaler

   Article

   Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

   Insert

   HSV-1 UL50 codon optimized
   

   Use

   Tags

   HA-Strep-Strep

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Gr4-T2A-QF2 HDR plasmid

   Plasmid

   #140944

   Purpose

   Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Gr4 gene

   Depositor

   Leslie Vosshall

   Article

   Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12

   Insert

   Gr4-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Gr4-right-HDR-arm
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. KN801: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-VPR

   Plasmid

   #121829

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the VPR transcriptional activator for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   x-dCas9(3.7)/VPR (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. KN901: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-KRAB

   Plasmid

   #121830

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the KRAB transcriptional repressor for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   x-dCas9(3.7)/KRAB (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. KO001: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-p300core

   Plasmid

   #121832

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the p300 catalytic core for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   x-dCas9(3.7)/p300core (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. p3E-DysF

   Plasmid

   #109544

   Purpose

   Multisite gateway vector for 3' tagging with Dysferlin

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   Dysferlin (dysf Zebrafish)
   

   Use

   Zebrafish plasmids

   Tags

   Expression

   Mutation

   Please see Depositor Comments

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5FRTTO-NHASt-codoptUL23

   Plasmid

   #135104

   Purpose

   Codon-optimized HSV-1 UL23 with N-terminal HAStrep tag

   Depositor

   Markus Landthaler

   Article

   Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

   Insert

   HSV-1 UL23 codon optimized
   

   Use

   Tags

   HA-Strep-Strep (N terminal on backbone)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5FRTTO-codoptUL23-CStHA

   Plasmid

   #135099

   Purpose

   Codon-optimized HSV-1 UL23 with C-terminal StrepHA tag

   Depositor

   Markus Landthaler

   Article

   Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

   Insert

   HSV-1 UL23 codon optimized
   

   Use

   Tags

   Strep-Strep-HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. PX458_GABPB1_iso2_1

   Plasmid

   #104041

   Purpose

   Encodes gRNA for 3' target of human GABPB1_iso2 along with Cas9 with 2A GFP

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   GABPB1_iso2 gRNA (GABPB1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFETCh_GABPB1_iso2

    Plasmid

    #104035

    Purpose

    Donor vector for 3' FLAG tag of human GABPB1_iso2

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    GABPB1_iso2 homology arms (GABPB1 Human)
    

    Use

    CRISPR

    Tags

    3XFLAG-P2A-NeoR

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. actc1b-mKate2-ARF1-DN

    Plasmid

    #109536

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to dominant negative ARF1

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    ARF1 (arf1 Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    DN

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-1 (GB1204)

    Plasmid

    #75405

    Purpose

    Provides the A. thaliana U6-1 RNA polIII promoter as a level 0 GoldenBraid part

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

    Insert

    AtU6-1 promoter
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. PX458_RERE_1

    Plasmid

    #101073

    Purpose

    Encodes gRNA for 3' target of human RERE

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA against RERE (RERE Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. p3E-Clta

    Plasmid

    #109585

    Purpose

    Multisite gateway vector for 3' tagging with Clathrin light chain a

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    Clathrin light chain a (clta Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. JJ802: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold

    Plasmid

    #121812

    Purpose

    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. actc1b-mKate2-ARF6

    Plasmid

    #109537

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to ARF6

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    ARF6 (arf6a Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. actc1b-mKate2-ARF6-DN

    Plasmid

    #109538

    Purpose

    Muscle specific zebrafish expression of mKate2 fused to dominant negative ARF6

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    ARF6 (arf6a Zebrafish)
    

    Use

    Zebrafish plasmids

    Tags

    mKate2

    Expression

    Mutation

    DN

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. KL301: pMAGIC (R4-R3) NLS-(SrfI/PmeI)-NLS-p300core

    Plasmid

    #121839

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS/p300core scaffold enabling addition of dCas9 mutants into pMAGIC. dCas9 can be inserted via unique SrfI/PmeI restriction sites

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pUAST attB Orco (Y478A)

    Plasmid

    #73928

    Purpose

    UAS induced expression of Drosophila melanogaster Orco cDNA with 478 Tyrosine to Alanine amino acid change

    Depositor

    Leslie Vosshall

    Article

    Nakagawa et al PLoS One. 2012;7(3):e32372. doi: 10.1371

    Insert

    Orco (Y478A) (Orco Fly)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    changed 478 Tyrosine for Alanine

    Promoter

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAB26 (punc-17::GtACR2::mCerulean::βHK::Chrimson)

    Plasmid

    #214889

    Purpose

    Expresses the optogenetic tool BiPOLES in cholinergic neurons of C. elegans.

    Depositor

    Alexander Gottschalk

    Article

    Bergs et al Nat Commun. 2023 Apr 6;14(1):1939. doi:

    Insert

    GtACR2_mCerulean_bHK_Chrimson
    

    Use

    Tags

    mCerulean

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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