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pcDNA3-ApaLIE225A, K227A-HA

Plasmid

#211460

Purpose

Express mitochondrial localized ApaLI with E225A and K227A mutations

Depositor

Agnel Sfeir

Article

Fu et al Mol Cell. 2023 Oct 8:S1097-2765(23)00753

Insert

ApaLI-E225A-K227A

Tags

HA

Expression

Mammalian

Mutation

E225A, K227A

Promoter

CMV

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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RPO41 deltaN380

Plasmid

#205076

Purpose

RPO41 protein expression

Depositor

Smita Patel

Article

Paratkar et al J Biol Chem. 2011 May 6;286(18):16109-20

Insert

RPO41 (RPO41 Budding Yeast)

Expression

Bacterial

Mutation

Lacks N Terminal 380 amino acids

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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RPO41 deltaN296

Plasmid

#205075

Purpose

RPO41 protein expression

Depositor

Smita Patel

Article

Paratkar et al J Biol Chem. 2011 May 6;286(18):16109-20

Insert

RPO41 (RPO41 Budding Yeast)

Expression

Bacterial

Mutation

Lacks N Terminal 296 amino acids

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pHYX143

Plasmid

#202816

Purpose

Control plasmid expressing mScarlet-I fluorescent protein.

Depositor

Jean-Félix Dallery

Article

Geistodt-Kiener et al Metab Eng. 2023 Oct 18:S1096-7176(23)001

Insert

mScarlet-I

Expression

Yeast

Promoter

ScPCK1

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2488

Plasmid

#190373

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::IcaR-NLS_19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::IcaR-NLS_19St

Expression

Plant

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2487

Plasmid

#190372

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::AmtR-NLS_19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::AmtR-NLS_19St

Expression

Plant

Available Since

May 18, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2514

Plasmid

#190382

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::TetR-NLS_19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::TetR-NLS_19St

Expression

Plant

Available Since

April 4, 2023

Availability

Academic Institutions and Nonprofits only

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JAB3017

Plasmid

#190399

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::McbR-NLS-19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::McbR-NLS-19St

Expression

Plant

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2858

Plasmid

#190394

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::AmtR-TPL(RD)-19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::AmtR-TPL(RD)-19St

Expression

Plant

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2519

Plasmid

#190385

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::BetI-NLS_19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::BetI-NLS_19St

Expression

Plant

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2518

Plasmid

#190384

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::RpaR-NLS_19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::RpaR-NLS_19St

Expression

Plant

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2495

Plasmid

#190377

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::BetI-VP16_19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::BetI-VP16_19St

Expression

Plant

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2494

Plasmid

#190376

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::RpaR-VP16_19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::RpaR-VP16_19St

Expression

Plant

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2281

Plasmid

#190362

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::AmtR-ERF2(AD)-19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::AmtR-ERF2(AD)-19St

Expression

Plant

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2180

Plasmid

#190353

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::TetR-VP16-19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::TetR-VP16-19St

Expression

Plant

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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JAB2156

Plasmid

#190345

Purpose

Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proG1090::AmtR-VP16-19St)

Depositor

Jennifer Brophy, Jose Dinneny

Article

Brophy et al Science. 2022 Aug 12;377(6607):747-751.

Insert

proG1090::AmtR-VP16-19St

Expression

Plant

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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T7-10-QUAS-GFP_ColE1

Plasmid

#171657

Purpose

QUAS ten base pairs downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

T7-10-QUAS

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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T7-15-QUAS-GFP_ColE1

Plasmid

#171658

Purpose

QUAS fifteen base pairs downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

T7-15-QUAS

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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T7-TetO-QF_T7-LacO-TetR_p15A

Plasmid

#171659

Purpose

Expresses QF and/or TetR in BL21(DE3) E. coli according to the presence of aTc. Contains a p15A origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

T7-LacO-TetR-T7 Stop

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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T7-0-QUAS-GFP_ColE1

Plasmid

#171650

Purpose

QUAS directly downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

T7-0-QUAS

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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