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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 8,560 results for: Dos

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Showing: 3341 - 3360 of 8560 results

  1. pRK5_tAKARβ

    Plasmid
    #119914
    Purpose
    Mammalian expression of the tAKARβ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)
    Depositor
    Haining Zhong
    Publication
    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    tAKARβ
    Expression
    Mammalian
    Available since
    Feb. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    119914-plasmid-map-sequence-id-235137

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  2. pCAG-hNme2Cas9-NLS(SV40)-3xFLAG (KAC582)

    Plasmid
    #134331
    Purpose
    CAG promoter expression plasmid for human codon optimized Nme2Cas9 nuclease with C-terminal NLS (SV40) and 3x FLAG tag
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Publication
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized Nme2Cas9
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    134331-plasmid-map-sequence-id-267376

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  3. pPBC-G3-tdT

    Plasmid
    #62814
    Purpose
    pPBC-G3-tdT expresses PiggyBac inverted terminal repeat-flanked GCaMP3 and tdTomato under the control of the CAG promoter.
    Depositor
    Karen Wilcox
    Publication
    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do
    Insert
    ITR-CAG-GCaMP3-IRES-tdTomato-ITR
    Expression
    Bacterial and Mammalian
    Promoter
    CAG
    Available since
    May 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    62814-plasmid-map-sequence-id-121226

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  8. pMIA10.7

    Plasmid
    #214451
    Purpose
    BxbI landing-pad Tet-On-T2A-puro-pA pA-GFP-TRE3G donor. Use with BxbI expressing plasmid to swap payload into 4.721 landing-pad. Generates inducible cell line.
    Depositor
    Roger Foo
    Publication
    Autio et al Elife. 2024 Jan 2;13:e79592. doi: 10.755
    Inserts
    Tet-On 3G
    PuroR
    TRE3G CopGFP
    Use
    Bxbi donor
    Tags
    PuroR, T2A, and Tet-On 3G
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    214451-plasmid-map-sequence-id-431689

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  9. Tol2 8xSTAR-mNeonGreen. PGK-puro

    Plasmid
    #136255
    Purpose
    Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity
    Depositor
    Hugo Snippert
    Publication
    Oost et al Cell Rep. 2018 Feb 6;22(6):1600-1614. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    stem cell Ascl2 reporter, 8 repeats
    Use
    Tol2 transposase
    Tags
    mNeonGreen
    Expression
    Mammalian
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    136255-plasmid-map-sequence-id-312894

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  11. pBT3000

    Plasmid
    #122536
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a ER localized gene (tram-1) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Publication
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_CemOrange2::tram-1 (tram-1 Synthetic, Nematode)
    Expression
    Bacterial
    Promoter
    Pnhx-2
    Available since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122536-plasmid-map-sequence-id-238187

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  16. pJEP13-AAV-H1/TO-L-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82703
    Purpose
    AAV backbone with a full length H1/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Publication
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    H1/TO-L gRNA Expression Cassette Containing Tet2 gRNA
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Available since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82703-plasmid-map-sequence-id-158053

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  19. 2xCBX7CD-IFP2.0C

    Plasmid
    #215754
    Purpose
    Expresses double domain of CBX7 chromo domain fused to C-terminal part of IFP2.0 for H3K27me3 detection in BiAD sensors
    Depositor
    Albert Jeltsch
    Publication
    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    2xCBX7CD-IFP2.0C
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    April 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    215754-plasmid-map-sequence-id-429736

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Showing: 3341 - 3360 of 8560 results